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Liquid chromatography using cellulosic continuous stationary phases

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Chromatography

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 49))

Abstract

A novel type of continuous stationary phase based on fabric materials is described. This column packing utilizes the continuous character of a cellulose (cotton) stationary phase, and the chemistry of the derivatized forms of the adsorbent, to obtain separations of proteins and small molecules based on cation and anion exchange, hydrophobic interactions, and size. The mechanical stability of the stationary phase facilitates chromatographic velocities in excess of 70 cm min−1. The influence of eluent properties on the adsorption of sample proteins is discussed in this chapter. Sequential stepwise desorption is used to separate 100 μl mixtures of BSA, IgG, β-galactosidase, and insulin in 10 minutes or less, using 10 mm i.d. × 500 mm length columns.

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References

  1. Reisman HB (1988) Economic analysis of fermentation processes. CRC Press, Boca Raton

    Google Scholar 

  2. Sofer GK, Nystrom L-E (1989) Process chromatography. Academic Press, San Diego

    Google Scholar 

  3. Knight P (1989) Bio/Technology 7:777

    Google Scholar 

  4. Eble JE, Grob RL, Antle PE, Snyder LR (1987) J Chromatogr 384:254

    Google Scholar 

  5. Gibbs SJ, Lightfoot EN (1986) Ind Eng Chem Fundam 25:490

    Article  CAS  Google Scholar 

  6. Ding H, Yang M-C, Schisla D, Cussler EL (1989) AIChE J 35:814

    Article  CAS  Google Scholar 

  7. Osawa AE, Cooney CL (1989) Paper No. 3, MBTD Division, 198th American Chemical Society National Meeting, Sept 10–15, 1989, Miami Beach, FL

    Google Scholar 

  8. Sharma SC, Fort JT (1973) J Colloid Interface Sci 43:36

    Article  CAS  Google Scholar 

  9. Kiso Y, Jinno K, Nagoshi T (1986) J High Res Chromatogr Commun 9:763

    CAS  Google Scholar 

  10. Rowland SP, Wade CP, Bertoniere NR (1984) J Appl Polym Sci 29:3349

    Article  CAS  Google Scholar 

  11. Bertoniere NR, King WD (1989) Textile Res J 59:114

    CAS  Google Scholar 

  12. Tiselius A (1955) Angew Chem 67:245

    CAS  Google Scholar 

  13. Kwapinski JBG (1972) Methodology of immunochemical and immunological research. Wiley-Interscience, New York

    Google Scholar 

  14. McGarry JD (1986) In: Devlin TM (ed) Textbook of biochemistry with clinical correlations. John Wiley, New York

    Google Scholar 

  15. Stryer L (1981) Biochemistry. Freeman, New York

    Google Scholar 

  16. Watson JD, Tooze J, Kurtz DT (1983) Recombinant DNA. Freeman, New York

    Google Scholar 

  17. Rowland SP, Roberts EJ, Wade CP (1969) Textile Res J 39:530

    CAS  Google Scholar 

  18. Wadsworth LC, Daponte D (1985) In: Nevell TP, Zeronian H (ed) Cellulose chemistry and its applications, Ellis Horwood, Chichester, West Sussex, UK

    Google Scholar 

  19. Ladisch CM, Yang Y, Velayudhan A, Ladisch MR (1992) Textile Res J 62:36

    Google Scholar 

  20. Righetti PG, Caravaggio T (1976) J Chromatogr 127:1

    Article  CAS  Google Scholar 

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Author information

Author notes

  1. Yiqi Yang

    Present address: Division of Consumer Sciences, University of Illinois at Urbana-Champaign, 61801, Urbana, IL

Authors and Affiliations

  1. Department of Consumer Sciences and Retailing, Purdue University, 47907, West Lafayette, IN, USA

    Yiqi Yang & Christine M. Ladisch

  2. Laboratory of Renewable Resources Engineering, Purdue University, 47907, West Lafayette, IN, USA

    Ajoy Velayudhan & Michael R. Ladisch

  3. Department of Agricultural Engineering, Purdue University, 47907, West Lafayette, IN, USA

    Michael R. Ladisch

Authors
  1. Yiqi Yang
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  2. Ajoy Velayudhan
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  3. Christine M. Ladisch
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  4. Michael R. Ladisch
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Editor information

G. T. Tsao

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© 1993 Springer-Verlag

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Yang, Y., Velayudhan, A., Ladisch, C.M., Ladisch, M.R. (1993). Liquid chromatography using cellulosic continuous stationary phases. In: Tsao, G.T. (eds) Chromatography. Advances in Biochemical Engineering/Biotechnology, vol 49. Springer, Berlin, Heidelberg. https://doi.org/10.1007/BFb0046576

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  • DOI: https://doi.org/10.1007/BFb0046576

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-56440-9

  • Online ISBN: 978-3-540-47513-2

  • eBook Packages: Springer Book Archive

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