Abstract
It has been reported that Vacor, a rodenticide containing N-3-pyridylmethyl-N’-p-nitrophenyl urea, causes insulin-dependent diabetes mellitus. The pathomechanism of Vacor-induced diabetes mellitus has not been clarified yet. The effect of Vacor, therefore, was studied in terms of insulin release from isolated rat pancreatic islets. Vacor suppressed glucose-stimulated insulin release, but did not affect the insulin release induced by theophylline or 12-o-tetra-decanoylphorbol 13-acetate. It is suspected that the suppression of insulin release from pancreatic islets by Vacor may contribute to the pathogenesis of Vacor-induced diabetes mellitus and that this suppression might not be related to cAMP and C-kinase.
This is a preview of subscription content, log in via an institution to check access.
References
Prosser P.R., Karam J.H. Diabetes mellitus following rodenticide ingestion in man. JAMA 239: 1148, 1978.
Miller L.V., Stokes J.D., Silpipat C. Diabetes mellitus and autonomic dysfunction after Vacor rodenticide ingestion. Diabetes Care 1: 73, 1978.
Pont A., Rubino J.M., Bishop D., Peal R. Diabetes mellitus and neuropathy following Vacor ingestion in man. Arch. Intern. Med. 139: 185, 1979.
Lee T.H., Kang J.C., Han M.D., Kim J.S., Roh J.W., Chung M.Y., Choi B.J. A clinical analysis of rodenticide “RH-787” intoxication in Korea. J. Korean Diab. Assoc. 4: 43, 1977.
Kang M.W., Kim C.S., Song J.W., Bang B.K., Kim K.H., Choi Y.K., Min B.S. The serum C-peptide levels in rodenticide RH-787 induced diabetes. Korean J. Intern. Med. 21: 27, 1978.
Lee T.H., Lee M.W. Inhibitory effect of rodenticide RH-787 on the glucose metabolism of erythrocytes. Korean J. Intern. Med. 20: 597, 1977.
Lee T.H. Immunoreactive insulin and C-peptide responses to oral glucose loads in the patients with RH-787 intoxication. Korean J. Intern. Med. 21: 507, 1978.
Karam J.H., Lewitt P.A., Young C.W., Nowlain R.E., Frankel B.J., Fujiya H., Freedman Z.R., Grodsky G.M. Insulinopenic diabetes after rodenticide (Vacor) ingestion. A unique model of acquired diabetes in man. Diabetes 29: 971, 1980.
Taniguchi H., Ejiri K., Ishihara K., Baba S. Methods of pancreatic islet isolation and culture and functional evaluation of cultured islet. Metab. Dis. 19: 99, 1982.
Morgan C.R., Lazarow A. Immunoassay of insulin: two antibody systems. Plasma insulin levels of normal, subdiabetic and diabetic rat. Diabetes 12: 115, 1963.
Karam J.H., Prosser P.R., LeWitt P.A. Islet-cell surface antibodies in a patient with diabetes mellitus after rodenticide ingestion. N. Engl.J. Med. 299: 1191, 1978.
Kenny R.M., Michaels I.A.L., Flomenbaum N.E., Yu G.S.M. Poisoning with N-3-pyridylmethyl-N’-p-nitrophenylurea (Vacor). Immunoperoxidase demonstration of β-cell destruction. Arch. Pathol. Lab. Med. 105: 367, 1981.
Fretthold D., Sunshine I., Udinsky J.R., Deckert F.W. Postmorterm findings for a Vacor poisoning case. Clin. Toxicol. 16: 175, 1980.
Brisson G.R., Malaisse-Lagae F., Malaisse W.J. The stimulus-secretion coupling of glucose-induced insulin release. A proposed site of action for adenosine-3′,5′-cyclic monophosphate. J. Clin. Invest. 51: 232, 1972.
Brisson G.R., Malaisse W.J. The stimulus-secretion coupling of glucose-induced insulin release. Effect of theophylline and epinephrine on 45Ca efflux from perifused islets. Metabolism 22: 455, 1973.
Zawalich W., Brown C., Rasmussen H. Insulin secretion: Combined effects of phorbol ester and A23187. Biochem. Biophys. Res. Commun. 117: 448, 1983.
Hutton J.C., Peshavaria M., Brocklehurst K.W. Phorbol ester stimulation of insulin release and secretory-granule protein phosphorylation in a transplan-table rat insulinoma. Biochem. J. 224: 483, 1984.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Taniguchi, H., Yamashiro, Y., Chung, M.Y. et al. Vacor inhibits insulin release from islets in vitro . J Endocrinol Invest 12, 273–275 (1989). https://doi.org/10.1007/BF03349985
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03349985