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TSH receptor gene expression in retroocular fibroblasts

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Abstract

RNA was isolated from fibroblasts from the retroocular area, from endomysial fibroblasts obtained from orbital lateral rectus muscle, and from abdominal skin fibroblasts. The RNA was reverse transcribed into cDNA which was then used as a template for PCR with primers encompassing a portion (nucleotides 989–1235) of the extra-cellular domain of the human TSH receptor (hTSH-R). A definite 247 BP product was detected from fibroblast RNA by ethidium bromide staining, and was confirmed by hybridization with labelled hTSH-R cDNA. The product had homology with the known TSH-R cDNA. These studies indicate that human fibroblasts can express hTSH-R, and they suggest that a cross reactive immunologic response between anti-hTSH-R and these fibroblast TSH receptors may play a role in the genesis of Graves’ ophthalmopathy.

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References

  1. Burman K.D., Baker J.R. Jr. Immune mechanisms in Graves’ disease. Endocr. Rev. 6: 183, 1985.

    Article  CAS  PubMed  Google Scholar 

  2. Wall J.R., Salvi M., Bernard N.F., Boucher A., Haegert D. Thyroid associated ophthalmopathy: a model for the association of organ — specific autoimmune disorders. Immunol. Today. 12: 150, 1991.

    Article  CAS  PubMed  Google Scholar 

  3. Heufelder A.E., Smith T.J., Gorman C.A., Bahn R.S. Increased induction of HLA-DR by interferon-gamma in cultured fibroblasts derived from patients with Graves’ ophthalmopathy and pretibial dermopathy. J. Clin. Endocrinol. Metab. 73: 307, 1991.

    Article  CAS  PubMed  Google Scholar 

  4. Heufelder A.E., Goellner J.R., Wenzel B.J., Bahn R.S. Immunohistochemical detection and localization of a 72 - kilodalton heat shock protein in autoimmune thyroid disease. J. Clin. Endocrinol. Metab. 74: 724, 1992.

    Article  CAS  PubMed  Google Scholar 

  5. Jacobson D.H., Gorman C.A. Endocrine ophthalmopathy: current ideas concerning etiology, pathogenesis and treatment. Endocr. Rev. 5: 200, 1984.

    Article  CAS  PubMed  Google Scholar 

  6. Parmentier M., Libert F., Maenhaut C., Lefort A., Gerard C., Perret J., Van Sande J., Dumont J.E., Vassart G. Molecular cloning of the thyrotropin receptor. Science 246: 1620, 1989.

    Article  CAS  PubMed  Google Scholar 

  7. Misrahi M., Loosfelt H., Atger M., Sar S., Guiochon-Mantel A., Milgrom E. Cloning, sequencing and expression of the human TSH receptor. Biochem. Biophys. Res. Commun. 166: 394, 1990.

    Article  CAS  PubMed  Google Scholar 

  8. Nagayama Y., Kaufman K., Rapoport B. Molecular cloning, sequence and functional expression of the cDNA for the human thyrotropin receptor. Biochem. Biophys. Res. Commun. 165: 1184, 1989.

    Article  CAS  PubMed  Google Scholar 

  9. Feliciello A., Porcellini A., Ciullo I., Bonavolonta G., Avvedimento E.V., Fenzi G. Expression of thyrotropin-receptor mRNA in healthy and Graves’ disease retro-orbital tissue. Lancet 342: 337, 1993.

    Article  CAS  PubMed  Google Scholar 

  10. Paschke R., Elisei R., Vassart G., Ludgate M. Lack of evidence supporting the presence of mRNA for the thyrotropin receptor in extra-ocular muscle. J. Endocrinol. Invest. 16: 329, 1993.

    Article  CAS  PubMed  Google Scholar 

  11. Endo T., Ohno M., Kotani S., Gunji K., Onaya T. Thyrotropin Receptor in Non-thyroid Tissues. Biochem. Biophys. Res. Commun. 190: 774, 1993.

    Article  CAS  PubMed  Google Scholar 

  12. Heufelder A.E., Dutton C.M., Sarkar G., Donovan K.A., Bahn R.S. Detection of TSH receptor RNA in cultured fibroblasts from patients with Graves’ ophthalmopathy and pretibial dermopathy. Thyroid 3: 297, 1993.

    Article  CAS  PubMed  Google Scholar 

  13. Heufelder A., Bahn R.S. Evidence for the presence of a functional TSH-receptor in retroocular fibroblasts from patients with Graves’ ophthalmopathy. Exp. Clin. Endocrinol. 100: 62, 1992.

    Article  CAS  PubMed  Google Scholar 

  14. Perros P., Kendall-Taylor P. TSH-binding sites are present in orbital connective tissue and are required by IgG from patients with thyroid — associated ophthalmopathy. Thyroid (Supplement 1, 2): 534 (abstract), 1992.

    Google Scholar 

  15. Molnar I., Balazs C. TSH binding site structures in human eye muscle fractions identified by using covalent-crosslinking. Biomed. Pharmacother. 46: 121, 1992.

    Article  CAS  PubMed  Google Scholar 

  16. Nagayama Y., Rapoport B. The thyrotropin receptor 25 years after its discovery: new insights after its molecular cloning. Mol. Endocrinol. 6: 145, 1992.

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. COL, Endocrine-Metabolic Service, 7D, Kyle Metabolic Unit, Department of Clinical Investigation, Walter Reed Army Medical Center, Washington, D.C., 20307-5001, USA

    M. Mengistu, Y. G. Lukes, E. V. Nagy, H. B. Burch, F. E. Carr, S. Lahiri & Kenneth D. Burman M.D.

  2. Department of Medicine, Georgetown University Medical Center, Washington, D.C., USA

    M. Mengistu, Y. G. Lukes, E. V. Nagy, H. B. Burch, F. E. Carr, S. Lahiri & Kenneth D. Burman M.D.

Authors
  1. M. Mengistu
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  2. Y. G. Lukes
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  3. E. V. Nagy
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  4. H. B. Burch
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  5. F. E. Carr
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  6. S. Lahiri
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  7. Kenneth D. Burman M.D.
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Deceased

The opinions or assertions contained herein are the private views of the authors and not to be construed as official or as reflecting the views of the Department of the Army or the Department of Defense.

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Mengistu, M., Lukes, Y.G., Nagy, E.V. et al. TSH receptor gene expression in retroocular fibroblasts. J Endocrinol Invest 17, 437–441 (1994). https://doi.org/10.1007/BF03347732

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  • DOI: https://doi.org/10.1007/BF03347732

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