Skip to main content
SpringerLink
Log in

Analysis of Genetic Diversity in Pongamia [Pongamia pinnata (L) Pierrre] using AFLP Markers

  • Published:
Journal of Plant Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

In recent years, Pongamia has been considered as important renewable source of biodiesel, however not much molecular information is available in this species. Molecular characterization of this legume tree will enhance our understanding in improving the optimal yields of oil through breeding and enable us to meet the future demands for biodiesel. To assess the molecular genetic diversity in 46 Pongamia pinnata accessions collected from six different states of India, amplified fragment length polymorphism (AFLP) marker system was employed. Five AFLP primer combinations produced 520 discernible fragments, of which 502 (96.5%) were polymorphic. AFLP primer informativeness was estimated evaluating four parameters namely polymorphism information content (PIC), effective multiplex ratio (EMR), marker index (MI) and resolving power (RP). In total, 51 unique fragments were detected of which 19 unique fragments were observed with primer combination E-ACG / M-CTA. Although neighbour joining (NJ) method did not group accessions strictly according to their region of collection, a good level of genetic diversity was observed in examined germplasm. However, accessions collected from Karnataka showed comparatively higher diversity than accessions from other states. The diverse accessions identified in this study may be useful in Pongamia pinnata improvement to meet the future demands of biodiesel.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

AFLP:

Amplified fragment length polymorphism

EMR:

Effective multiplex ratio

FAME:

Fatty acid methyl esters

MI:

Marker index

PIC:

Polymorphism information content

RP:

Resolving power

SVO:

Straight vegetable oil

References

  1. Wani SP, Osman M, Emmanuel D'Silva & Sreedevi TK, Asian Biotechnol Deve Rev, 6 (2006) 11.

    Google Scholar 

  2. Scott PT, Pregelj L, Chen N & Gresshoff PM, Bioenergy Research, 1 (2008) 2.

    Article  Google Scholar 

  3. Atchison E, Am J Bot, 36 (1951) 538.

    Article  Google Scholar 

  4. Sarbhoy RK, Cytologia, 42 (1977) 415.

    Article  Google Scholar 

  5. Kumar V, Chandrashekar K & Sidhu OP, J Entomol Res, 30 (2006) 103.

    Google Scholar 

  6. Khurma UR & Mangotra A, S Pac J Nat Sci, 22 (2004) 50.

    Google Scholar 

  7. Yadav YS, Siddiqui AU & Parihar A, J Phytol Res, 16 (2005) 263.

    Google Scholar 

  8. Elanchezhiyan M, Rajarajan S, Rajendran P, Subramanian S & Thyagarajan SP, J Mad Microbiol, 36 (1993) 262.

    Article  Google Scholar 

  9. D'sllva E, Wani SP & Nagnath B, Patancheru 502 324, Global theme on Agroecosystems, Report No. 11 (2004) 28.

    Google Scholar 

  10. Azam MM, Waris A & Nahar NM, Biomass and Bioenergy, 29 (2005) 293.

    Article  Google Scholar 

  11. Karmee SJ & Chadha A, Biores Technol, 96 (2005) 1425.

    Article  CAS  Google Scholar 

  12. Nagaraj G & Mulda N, J Oilseeds Res, 21 (2004) 117.

    Google Scholar 

  13. Natanam R, Kadirvel R & Chandrasekaran D, Animal Feed Sci Technol, 25 (1989) 201.

    Article  Google Scholar 

  14. Rathore V & Madras G, Fuel, 66 (2007) 2650.

    Article  Google Scholar 

  15. Bena G, Jubler MF, Olivieri I & Lejeune B, J Mot Evol, 46 (1998) 299.

    Article  CAS  Google Scholar 

  16. Parani M, Lakshmi M, Kumar SP & Parida A, Curr Sci, 76 (2000) 1235.

    Google Scholar 

  17. Casiva PV, Saidman BO, Vilardi VC & Cialdella AM, Am J Bot, 69 (2002) 843.

    Article  Google Scholar 

  18. Vos P, Hogers R, Bleaker M, Reijans M, Lee T, Homes M, Friters A, Pot J, Paleman J, Kulper M & Zabeau M, Nucleic Acids Res, 23 (1995) 4407.

    Article  PubMed  CAS  Google Scholar 

  19. Tatikonda L, Wani SP, Kannan S, Beerelli N, Holsington DA, Devi P & Varshney RK, Plant Sci, 176 (2009) 505.

    Article  CAS  Google Scholar 

  20. Roldan-Rulz J, Dendauw E, VanBockstaele A, Depicker M & Loose De, Mot Breed, 6 (2000) 125.

    Article  Google Scholar 

  21. Varshney RK, Chabane K, Hendre PS & Aggarwal RK, Plant Sci, 73 (2007) 638.

    Article  Google Scholar 

  22. Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S & Rafalski A, Mot Breed, 2 (1996) 225.

    Article  CAS  Google Scholar 

  23. Prevost A & Wilkinson MJ, Theor Appl Genet, 96 (1999) 107.

    Article  Google Scholar 

  24. Nei M, American Naturalist, 106 (1972) 283.

    Article  Google Scholar 

  25. Swofford DL, Phylogenetic analysis using Parsimony (*and Other Methods) Version 4, Sinauer Associates, Sunderland, Massachusetts, (2003).

    Google Scholar 

  26. Hudson DH, Richter DC, Rausch C, Dezulian T, Franz M & Rupp R, BMC Biointormatics, 6 (2007) 460.

    Article  Google Scholar 

  27. Milbourne D, Meyer R, Bradshaw J, Baird E, Boner N, Proven J, Powell W & Waugh R, Mot Breed, 3 (1997) 127.

    Article  CAS  Google Scholar 

  28. Russell JR, Fuller JD, Macaulay M, Hatz BG, Jahoor A, Powell W & Waugh R, Theor Appl Genet, 95 (1997) 714.

    Article  CAS  Google Scholar 

  29. Sun Q-B, Li L-F, Li Y, Wu G-J & Ge X-J, Crop Sci, 46 (2008) 1865.

    Article  Google Scholar 

  30. Fu Yong-Bi, Gugel RK & Katepa-Mupondwa F, Plant Gen Res, 4 (2006) 87.

    Article  CAS  Google Scholar 

  31. Rosales-Serna R, Herna'ndez-Delgado S, Gonza’ lez-Paz M, Acosta-Gallegos JA & Mayek-Pe'rez N, Crop Sci, 45 (2005) 1951.

    Article  CAS  Google Scholar 

  32. Fernandez M, Figueiras A & Benito C, Theor Appl Genet, 104 (2002) 845.

    Article  PubMed  CAS  Google Scholar 

  33. Laurentin H & Karlovsky P, Genet Resour Crop Evol, 54 (2007) 1437.

    Article  Google Scholar 

  34. Gupta S, Srivastava M, Mishra G.P, Naik PK, Chauhan RS, Tiwari SK, Kumar M & Singh R, Atr J Biotechnol, 7 (2008) 4230.

    CAS  Google Scholar 

  35. Bohn M & Utz H, Crop Sci, 39 (1999) 228.

    Article  CAS  Google Scholar 

  36. Srivastava A, Gupta V, Pental D & Pradhan AK, Theor Appl Genet, 102 (2001) 193.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. International Crops Research Institute for the Semi-Arid Tropics, Patancheru, Greater Hyderabad, 502 324, Andhra Pradesh, India

    Mahendar Thudi, Revathi Manthena, Suhas P. Wani, Leela Tatikonda, David A. Hoisington & Rajeev K. Varshney

  2. Osmania University, Tamaka, Greater Hyderabad, 500 007, Andhm Pradesh, India

    Leela Tatikonda

  3. Genomics Towards Gene Discovery Subprogmmme, Generation Challenge Programme (GCP), CIMMYT Int APDO Postal 6-641, 06600, Mexico DF, Mexico

    Rajeev K. Varshney

Authors
  1. Mahendar Thudi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Revathi Manthena
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Suhas P. Wani
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Leela Tatikonda
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. David A. Hoisington
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Rajeev K. Varshney
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Rajeev K. Varshney.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Thudi, M., Manthena, R., Wani, S.P. et al. Analysis of Genetic Diversity in Pongamia [Pongamia pinnata (L) Pierrre] using AFLP Markers. J. Plant Biochem. Biotechnol. 19, 209–216 (2010). https://doi.org/10.1007/BF03263342

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03263342

Key words

Search

Navigation