Abstract
The full-length enolase-encoding cDNA was cloned from cotton (Gossypium barbadense). This gene (GenBank Accession No.: AY297757) had a total length of 1580 bp with an open reading frame of 1338 bp and encoded a predicted polypeptide of 445 amino acid residues with a molecular weight of 47.73 kD. Comparison of it primary structure with those of other enolases revealed a remarkable degree of conservation, except for the presence of an insertion of 5 amino acid residues unique to higher plant enolases. Southern blotting analysis of genomic DNA indicated enolase was likely to be a low-copy gene in the cotton genome. The enolase gene was induced in response to submergence, ABA, salt and high temperature stress. Our studies suggested that the cloned gene was a new member of plant enolase gene family, which contributed to the energy supply in stress-treated tissues.
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Kaijnq Zuo and Jingya Zhao contributed equally to this paper
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Zuo, K., Zhaoa, J., Sun, X. et al. cDNA Cloning and Characterization of Enolase from Cotton (Gossypium barbadense). J. Plant Biochem. Biotechnol. 14, 107–114 (2005). https://doi.org/10.1007/BF03263237
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DOI: https://doi.org/10.1007/BF03263237