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Genetic and Biotechnological Approaches for Reducing Glucosinolates from Rapeseed-Mustard Meal

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Abstract

The seed meal of oleiferous brassicas is an excellent feed concentrate whose nutritional value is impaired by glucosinolates. Glucosinolates and the products of their hydrolytic cleavage are toxic, goitrogenic and reduce palatability because of their pungency. Efforts to develop low-glucosinolate varieties have not been very successful since the genetics of total glucosinolate biosynthesis is complex and highly regulated. This review summarizes information from genetic and plant breeding studies on identification and mapping of the genetic determinants for glucosinolate biosynthesis in Brassicas. Additional options offered by plant biotechnology using genetic engineering tools such as antisense technology and metabolic pathway engineering that could bring down the seed glucosinolate levels have also been highlighted.

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References

  1. Ettlinger MG & Lundeen AJ, J Am Chem Soc, 78 (1956) 1952.

    Article  CAS  Google Scholar 

  2. Ettlinger MG & Lundeen AJ, J Am Chem Soc, 79 (1957) 1764.

    Article  CAS  Google Scholar 

  3. Sorensen H, In Canola and rapeseed: Production, chemistry, nutrition and processing technology (F Shahidi, Editor), Van Nostrand Reinhold, NY (1990) p 149.

    Google Scholar 

  4. Robbelen G, In Production and utilization of protein in oilseed crops (ES Bunting, Editor), Nijhoff-Junk, The Hague (1980) p 90.

    Google Scholar 

  5. Olson O & Sorensen H, J Am Oil Chem Soc, 58 (1981) 857.

    Article  Google Scholar 

  6. Natruzzi C, Cortesti R, Esposito E, Menegatti E, Leoni O, lori R & Palmieri S, J Agri Food Chem, 44 (1996) 1014.

    Article  Google Scholar 

  7. MacCleod A & Islam R, J Sci Food Agri, 26 (1975) 1545.

    Article  Google Scholar 

  8. Cole R, Phytochem, 15 (1976) 750.

    Article  Google Scholar 

  9. Cole R, J Sci Food Agri, 31 (1980) 549.

    Article  CAS  Google Scholar 

  10. Kjaer A, Ogaard J & Maeda Y, Phytochem, 17 (1978) 1285.

    Article  CAS  Google Scholar 

  11. Daxenbichler ME, Spencer GF, Carlson DG, Rose GB, Brinker AM & Powell RG, Phytochem, 30 (1991) 2623.

    Article  CAS  Google Scholar 

  12. Heaney RK Spinks EA, Hanley AB & Fenwick GR, In Analysis of glucosinolates in rapeseed: Technical bulletin, AFRC Food Research Institute, Norwich (1986).

    Google Scholar 

  13. Grob K & Matile P, Phytochem, 19 (1980) 1789.

    Article  CAS  Google Scholar 

  14. Fenwick GR, Heaney RK & Mullin WJ, Crit Rev Food Sci Nutr, 18 (1983) 123.

    PubMed  CAS  Google Scholar 

  15. Maheshwari PN, Stanley DW, Gray JI & van de Voort FR, J Am Oil Chem Soc, 56 (1979) 837.

    Article  CAS  Google Scholar 

  16. Josefsson E & Akerstrom L, J Chromatogr, 174 (1979) 465.

    Article  PubMed  CAS  Google Scholar 

  17. McLeod HA, Benns G, Lewis D & Lawrence JF, J Chromatogr, 157 (1978) 285.

    Article  PubMed  CAS  Google Scholar 

  18. Benns G, L’Abbe MR & Lawrence JF, J Agr Good Chem, 27 (1979) 426.

    Article  CAS  Google Scholar 

  19. Helboe P, Olsen O & Sorensen H, J Chromatogr, 197 (1980) 199.

    Article  CAS  Google Scholar 

  20. Betz JM & Page SW, Plant Med, 56 (1990) 590.

    Article  Google Scholar 

  21. Minchinton I, Sang J, Burke D & Truscott RJW, J Chromatogr, 247 (1982) 141.

    Article  CAS  Google Scholar 

  22. Spinks EA, Fenwick GR & Edwards WTE, Cruciferae Newslett, 8 (1983) 48.

    Google Scholar 

  23. Sang JP & Truscott RJW, J Am Off Anal Chem, 67 (1984) 829.

    CAS  Google Scholar 

  24. Quinsac A & Ribaillier D, In Proceedings of the 7th International Rapeseed Congress, Poland (1987) 1354.

    Google Scholar 

  25. Quinsac A, Ribaillier D, Elfakir C, Lafosse M & Dreux M, J Am Off Anal Chem, 774 (1991) 932.

    Google Scholar 

  26. Michaelsen S, Moller P & Sorensen H, J Chromatogr, 608 (1992) 363.

    Article  CAS  Google Scholar 

  27. Morin Ph, Villard F, Quinsac A & Dreux M, J High Resolution Chrom Chrom Commun, 15 (1992) 271.

    Article  CAS  Google Scholar 

  28. Feldl C, Moller P, Otte J & Sorensen H, Anal Biochem, 217 (1994) 62.

    Article  PubMed  CAS  Google Scholar 

  29. Fenwick GR, Eagles J & Self R, Organic Mass Spectrosc, 17 (1982) 544.

    Article  CAS  Google Scholar 

  30. McGregor DI, Mullin WH & Fenwick GR, J AM Off Anal Chem, 66 (1983) 825.

    CAS  Google Scholar 

  31. Kore AM, Spencer GF & Wallig MA, J Agr Food Chem, 41 (1993) 89.

    Article  CAS  Google Scholar 

  32. Bojesen G & Larsen E, Biol Mass Spectrosc, 20 (1991) 286.

    Article  CAS  Google Scholar 

  33. Kokkonen PS, van der Greef J, Niessen WMA, Tjaden UR, ten Hove GJ & van de Werken G, Biol Mass Spectrosc, 20 (1991) 259.

    Article  CAS  Google Scholar 

  34. Smith DR, Olivares JA, Nguyen NT & Udseth HR, Anal Chem, 60 (1988) 436.

    Article  CAS  Google Scholar 

  35. Rosa EAS, Heaney RK, Fenwick Gr & Portas CAM, Hort Rev, 19 (1997) 99.

    CAS  Google Scholar 

  36. Visentin M, Tava A, lori R & Palmieri S, J Agr Food Chem, 40 (1992) 1687.

    Article  CAS  Google Scholar 

  37. Larsen PO, In The biochemistry of plants A comprehensive treatise, Vol 7 (EE Conn, Editor). Academic Press, NY (1981) P 502.

    Google Scholar 

  38. Underhill EW, In Encyclopedia of plant physiology, Vol 8 (EA Bell, BV Charlwood, Editors), Springer-Verlag, NY (1980) p 493.

    Google Scholar 

  39. Underhill EW, Wetter LR & Chisolm MD, Biochem Soc Symp, 38 (1973) 303.

    Google Scholar 

  40. Poulton JE & Moller BL, In Methods in plant biochemistry, Vol 9 (PJ Lea, Editor), Academic Press, London (1993) p 209.

    Google Scholar 

  41. Walisgrove AM & Bennett AN, In Amino acids and their derivatives in higher plants (AM Walisgrove, Editor), Cambridge University Press, Cambridge (1995) p 243.

    Chapter  Google Scholar 

  42. Chapple CCS, Glover GR & Ellis BE, Plant Physiol, 94 (1990) 1887.

    Article  PubMed  CAS  Google Scholar 

  43. Haughn GW, Davin L, Giblin M & Underhill EW, Plant Physiol, 97 (1991) 217.

    Article  PubMed  CAS  Google Scholar 

  44. Dawson GW, Hick AJ, Bennett AN, Donald AM & Wallsgrove RM, J Biol Chem, 268 (1993) 27154.

    PubMed  CAS  Google Scholar 

  45. Bennett AN, Donald AM, Dawson GW, Hick AJ & Walisgrove RM, Plant Physiol, 102 (1993) 1307.

    PubMed  CAS  Google Scholar 

  46. Bennett AN, Ludwig-Muller J, Kiddle G, Hilgenberg W & Walisgrove RM, Planta, 196 (1995) 239.

    Article  CAS  Google Scholar 

  47. Bennett AN, Dawson GW, Hick AJ & Wallsgrove RM, Plant Physiol, 109 (1995) 299.

    PubMed  CAS  Google Scholar 

  48. Ziegler DM, Drug Metabolism Rev, 19 (1988) 1.

    Article  CAS  Google Scholar 

  49. Coecke S, Mertens K, Segaert A, Callaerts A, Vercruysse A & Rogiers V, Anal Biochem, 205 (1992) 285.

    Article  PubMed  CAS  Google Scholar 

  50. Ludwig-Muller J & Hilgenberg W, Physiol Plant, 74 (1988) 240.

    Article  Google Scholar 

  51. Bennett AN, Kiddle G, Hick AJ, Dawson GW & Wallsgrove RM, Plant Cell Environ, 19 (1996) 801.

    Article  CAS  Google Scholar 

  52. Rossiter JT, James DC & Atkins N, Phytochem, 29 (1990) 2509.

    Article  CAS  Google Scholar 

  53. Teutsch HG, Hasenfratz MP, Lesot A, Stoltz C, Garnier J-M, Jeltsch J-M, Durst F & Werck-Reichhart D, Proc Natl Acad Sci, USA, 90 (1993) 4102.

    Article  PubMed  CAS  Google Scholar 

  54. Sang JP & Salisbury PA, J Sci Food Agr, 45 (1988) 255.

    Article  CAS  Google Scholar 

  55. Downey RK & Craig BM, J Am Oil Chem Soc, 41 (1964) 475.

    Article  CAS  Google Scholar 

  56. Downey RK, Plant Breed Abstr, 10 (1990) 1165.

    Google Scholar 

  57. Downey RK & Rimmer SR, Adv Agron, 50 (1993) 1.

    Article  Google Scholar 

  58. Downey RK & Robbelen G, In Oil crops of the world (G Robbelen, RK Downey, A Ashri, Editors), McGraw Hill, New York (1989) P 339.

    Google Scholar 

  59. Chen BY & Heneen WK, Cruciferae Newslett, 18 (1996) 82.

    CAS  Google Scholar 

  60. Chapple C, In Recent advances in phytochemistry (BE Ellis, GW Kuroki, HA Stafford, Editors), Vol 8 (1994) p 251.

    Google Scholar 

  61. Chapple CCS, Shirley BW, Zook M, Hammerschmidt R & Somerville CS, In Arabidopsis (EM Meyerowitz, CR Somerville, Editors), Cold Spring Harbor Monograph Series 27 (1994) p 989.

    Google Scholar 

  62. Toroser D, Wood C, Griffiths H & Thomas DR, J Exp Bot, 46 (1995) 787.

    Article  CAS  Google Scholar 

  63. Zhao FJ, Bilsborrow PE, Evans EJ & Syers JK, J Sci Food Agric, 62 (1993) 135.

    Article  Google Scholar 

  64. Bilsborrow PE, Evans EJ, Murray F & Zhao FJ, Annal Appl Biol, 122 (1993) 135.

    Article  CAS  Google Scholar 

  65. Toroser D, Thormann CE, Osborn TC & Mithen R, Theor Appl Genet, 91 (1995) 802.

    Article  CAS  Google Scholar 

  66. Mithen R, Clarke J, Lister C & Dean C, Heredity, 74 (1995) 210.

    Article  CAS  Google Scholar 

  67. Giamoustaris A & Mithen R, Theor Appl Genet, 93 (1996) 1006.

    Article  CAS  Google Scholar 

  68. Mithen R, Lewis BG & Fenwick GR, Phytochem, 26 (1987) 1969.

    Article  CAS  Google Scholar 

  69. Magrath R, Bano F, Morgner M, Parkin I, Sharpe A, Lister C, Dean C, Turner J, Lydiate D & Mithen R, Heredity, 72 (1994) 290.

    Article  CAS  Google Scholar 

  70. Duescher RJ, Lawton MP, Philpot AM & Elfarra AA, J Biol Chem, 269 (1994) 17525.

    PubMed  CAS  Google Scholar 

  71. Kidd G, Bio/Technol, 11 (1993) 448.

    Article  Google Scholar 

  72. Ibrahim RK, Chavadej S & de Luca V, In Recent advances in phytochemistry (BE Ellis, GW Kuroki, HA Stafford, Editors), Vol 8 (1994) p 125.

    Google Scholar 

  73. Chavadej S, Brisson N, McNeil JN & de Luca V, Proc Natl Acad Sci, USA, 91 (1994) 2166.

    Article  PubMed  CAS  Google Scholar 

  74. Bodnaryk RP, Phytochem, 31 (1992) 2671.

    Article  CAS  Google Scholar 

  75. Bodnaryk RP, Phytochem, 35 (1994) 301.

    Article  CAS  Google Scholar 

  76. Kiddle GA, Doughty KJ & Wallsgrove RM, J Exp Biol, 45 (1994) 1343.

    CAS  Google Scholar 

  77. Doughty KJ, Kiddie G, Pye BJ, Wallsgrove AM & Pickett JA, Phytochem, 38 (1995) 347.

    Article  CAS  Google Scholar 

  78. Chew FS, In Biologically active natural products: Potential use in agriculture (HG Cutler, Editor), American Chemical Society, Washington DC (1988) p 155.

    Google Scholar 

  79. Glen DM, Jones H & Fieldsend JK, Annal Appl Biol, 117 (1990) 197.

    Article  CAS  Google Scholar 

  80. Koritsas VM, Lewis JA & Fenwick GR, Annal Appl Biol, 118 (1991) 209.

    Article  Google Scholar 

  81. Mithen R, Euphytica, 63 (1992) 71.

    Article  CAS  Google Scholar 

  82. Bennett AN & Walisgrove RM, New Phytol, 127 (1994) 617.

    Article  CAS  Google Scholar 

  83. Milford GFJ, Fieldsend JK, Porter AJR, Rawlinson CJ, Evans EJ & Bilsborrow PE, Aspect Appl Biol, 23 (1989) 83.

    Google Scholar 

  84. Porter AJR, Morton AM, Kiddle G, Doughty K & Walisgrove RM, Annal Appl Biol, 118 (1991) 461.

    Article  CAS  Google Scholar 

  85. Clossais-Besnard N & Lahrer F, J Sci Food Agri, 56 (1991) 25.

    Article  CAS  Google Scholar 

  86. Walisgrove RM, Bennett R, Donald A, Kiddle G, Porter A & Doughty K, Aspect Appl Biol, 34 (1993) 155.

    Google Scholar 

  87. Zhang Y, Talahay P, Cho C-G & Posner G. Proc Natl Acad Sci, USA, 89 (1992) 2399.

    Article  PubMed  CAS  Google Scholar 

  88. Zhang Y, Kensler T, Cho C-G, Posner G & Talahay P, Proc Natl Acad Sci, USA, 91 (1994) 3147.

    Article  PubMed  CAS  Google Scholar 

  89. Tawfiq N, Heaney R, Plumb J, Fenwick G, Musk S & Williamson G, Carcinogenesis, 16 (1995) 1191.

    Article  PubMed  CAS  Google Scholar 

  90. Block G, Patterson B & Subar A, Nutrition and Cancer, 18 (1992) 1.

    Article  PubMed  CAS  Google Scholar 

  91. Giamoustaris A & Mithen R, Annal Appl Biol, 126 (1995) 347.

    Article  CAS  Google Scholar 

  92. Giamoustaris A & Mithen R, Plant Pathol, 46 (1997) 45.

    Article  Google Scholar 

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Vageeshbabu, H.S., Chopra, V.L. Genetic and Biotechnological Approaches for Reducing Glucosinolates from Rapeseed-Mustard Meal. J. Plant Biochem. Biotechnol. 6, 53–62 (1997). https://doi.org/10.1007/BF03263011

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