Skip to main content
SpringerLink
Log in

Effect of sulfur on nitrate reductase and ATP sulfurylase Activities in groundnut (Arachis hypogea L.)

  • Short Communication
  • Published:
Journal of Plant Biology Aims and scope Submit manuscript

Abstract

Field experiments were conducted to determine the effect of sulfur (S) and Nitrogen (N) on nitrate reductase (NR) and ATP-sulfurylase activities in groundnut cultivars (Arachis hypogea L. cv. Ambar and Kaushal). Two combinations of S (in kg ha-1): OS (-S) and 20S (+S) were used with 20 kg ha-1 N. The application of S enhanced the NR and ATP-sulfurylase activities in both the cultivars at all the growth stages. The application of S also increased soluble protein and chlorophyll content in the all growth stages of both the cultivars. NR and ATP-sulfurylase activities in the leaves were measured at various growth stages as the two enzymes catalyze the rate limiting steps of the assimilatory pathways of nitrate and sulfate, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Literature Cited

  • Ahmad A, Abraham G, Abdin MZ (1999) Physiological investigation of the impact of nitrogen and sulfur application on seed and oil yield of rapeseed (Brassica campestris L.) and mustard (Brassica juncea L. Czern. and Coss.) genotypes. J Agron Crop Sci183: 19–25

    Article  CAS  Google Scholar 

  • Barney JPE, Bush LP (1985) Interaction of nitrate and sulfate reduction in tobacco: I. influence of availability of nitrate and sulphate. J Plant Nutr8: 507–515

    Google Scholar 

  • Blaszczyk A, Brodzik R Sirko A (1999) Increased resistance to oxidative stress in transgenic tobacco plants overexpressing bacterial serine acelytransferase. Plant J20: 237–243

    Article  PubMed  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem72: 248–254

    Article  PubMed  CAS  Google Scholar 

  • Brunold C, Suter M (1984) Regulation of sulfate assimilation by nitrogen nutrition in the duckweedLemna minor L. Plant Physiol76: 579–583

    Article  PubMed  CAS  Google Scholar 

  • Clarkson DT, Saker LR, Purves JV (1989) Depression of nitrate and ammonium influx in barley plants with diminished sulfate-status: evidence for co-regulation of nitrogen and sulfate intake. J Exp Bot40: 953–963

    Article  CAS  Google Scholar 

  • Clarkson DT, Saker LR, Purves JV, Lee RB (1989) Depression of nitrate and ammonium transport in barley plants with diminished sulphate status: Evidence of co-regulation of nitrogen and sulphate intake. J Expt Bot40: 953–963

    Article  CAS  Google Scholar 

  • Davies JP, Grossman AR (1998) Responses to deficiencies in macronutrients,In M Goldschmidt-Clermont, S Merchant, Eds, The Molecular Biology of Chloroplast and Mitochondria inChlamydomonas. Kluwer Academic, Amsterdam, pp 613–633

    Google Scholar 

  • Eppendorfer WH (1971) Effect of sulfur, nitrogen and phosphorus on the amino acid composition of the field bean (Vicia faba) and responses of the biological value of the seed protein and sulfur-amino acid content. J Sci Food Agric22: 501–505

    Article  PubMed  CAS  Google Scholar 

  • Ferreira RMB, Teixeira ARN (1992) Sulfur starvation inLemna leads to degradation of ribulose/bisphosphate carboxylase without plant death. J Biol Chem267: 6253–6257

    Google Scholar 

  • Gilbert SM, Clarkson DT, Cambridge M, Lambers H, Hawkesford MJ (1997) SO42 deprivation has an early effect on the content of ribulose-1, 5-bisphosphate carboxylase/oxygenase and photosynthesis in young leaves of wheat. Plant Physiol115: 1231–1239

    PubMed  CAS  Google Scholar 

  • Giordano M, Pezzoni V, Hell R (2000) Strategies for the allocation of resources under sulfur limitation in the green algaDunaliella salina. Plant Physiol124: 857–864

    Article  PubMed  CAS  Google Scholar 

  • Harms K, Ballmoos P, von Brunold C, Hofgen R, Hesse H (2000) Expression of a bacterial serine acetyltransferase in transgenic potato plants leads to increased levels of cysteine and glutathione. Plant J22: 335–343

    Article  PubMed  CAS  Google Scholar 

  • Hell R (1997) Molecular physiology of plant sulfur metabolism. Planta202: 138–148

    Article  PubMed  CAS  Google Scholar 

  • Hesse H, Trachsel N, Suter M, Kopriva S, Ballmoos P, von Rennenberg H, Brunold C (2003) Effect of glucose on assimilatory sulphate reduction inArabidopsis thaliana roots. J Exp Bot54: 1701–1709

    Article  PubMed  CAS  Google Scholar 

  • Hiscox JD, Israelstam GF (1979) A method for extraction of chlorophyll from leaf tissues without maceration. Can J Bot57: 1332–1334

    Article  CAS  Google Scholar 

  • Kim K, Hirai MY, Hayashi H, Chino M, Naito S, Fujiwara T (1999) Role of O-acetyl-l-serine in the coordinated regulation of the expression of a soybean seed storage-protein gene by sulfur and nitrogen nutrition. Planta209: 282–289

    Article  PubMed  CAS  Google Scholar 

  • Klepper LA, Flesher D, Hageman RH (1971) Potential for nitrate reduction in wheat (Triticum astivum L). J Plant Nutr3: 843–852

    Google Scholar 

  • Koprivova A, Suter M, Camp RO, den Brunold C, Kopriva S (2000) Regulation of sulfate assimilation by nitrogen inArabidopsis. Plant Physiol122: 737–746

    Article  PubMed  CAS  Google Scholar 

  • Kramer K, Schmidt A (1989) Nitrite accumulation bySynechococcus 6301 as a consequence of carbon or sulfur deficiency. FEMS Microbiol Lett59: 191–196

    Article  Google Scholar 

  • Leustek T, Martin MN, Bick JA, Davies JP (2000) Pathways and regulation of sulfur metabolism revealed through molecular genetic studies. Annu Rev Plant Physiol Plant Mol Biol51: 141–166

    Article  PubMed  CAS  Google Scholar 

  • Migge A, Bork C, Hell R (2000) Negative regulation of nitrate reductase gene expression by glutamine or asparagine accumulating in leaves of sulfur-deprived tobacco. Planta211: 587–595

    Article  PubMed  CAS  Google Scholar 

  • Nageswar RG (1983) Statistics for Agricultural Sciences. Oxford and IBH Publishing, Oxford

    Google Scholar 

  • Nair TVR, Abrol YP (1997) Studies on nitrate reducing system in developing wheat ears. Crop Sci17: 438–442

    Article  Google Scholar 

  • Prasser IM, Purves JV, Saker LR, Clarkson DT (2001) Rapid disruption of nitrogen metabolism and nitrate transport in spinach plants deprived of sulphate. J Exp Bot52: 113–121

    Article  Google Scholar 

  • Rennenberg H (1983) Role of O-acetylserine in hydrogen sulfide emission from pumpkin leaves in response to sulfate. Plant Physiol73: 560–565

    Article  PubMed  CAS  Google Scholar 

  • Reuveny Z, Dougall DK, Trinity PM (1980) Regulatory coupling of nitrate and sulfate assimilation pathways in cultured tobacco cells. Proc Natl Acad Sci USA77: 6670–6672

    Article  PubMed  CAS  Google Scholar 

  • Smith IK (1980) Regulation of sulfate assimilation in tobacco cells: effect of nitrogen and sulfur nutrition on sulfate permease and O-acetylserine sulfhydrylase. Plant Physiol66: 877–883

    Article  PubMed  CAS  Google Scholar 

  • Stewart BA, Porter LK (1969) Nitrogen-Sulphur relationships in wheat (Triticum aestivum L.), corn(Zea mays), and beans (Phaseolus vulgaris). Agron J61: 267–271

    CAS  Google Scholar 

  • Suter M, Lavanchy P, Arb C, von Brunold C (1986) Regulation of sulfate assimilation by amino acids inLemna minor L. Plant Sci44: 125–132

    Article  CAS  Google Scholar 

  • Wilson LG, Bandurski RS (1958) Enzymatic reactions involving sulphate, sulphite, selenate and molybdate. J Biol Chem233: 975–981

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, Faculty of Science, Jamia Hamdard, New Delhi-110062, India

    Arshad Jamal, Inayat Saleem Fazli, Saif Ahmad & Malik Zainul Abdin

  2. Horticultural Biotechnology Division, National Horticulture Research Institute, RDA, 441-440, Suwon, Korea

    Arshad Jamal, Ki-Taek Kim & Dae-Geun Oh

Authors
  1. Arshad Jamal
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Inayat Saleem Fazli
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Saif Ahmad
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ki-Taek Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Dae-Geun Oh
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Malik Zainul Abdin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Arshad Jamal.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jamal, A., Fazli, I.S., Ahmad, S. et al. Effect of sulfur on nitrate reductase and ATP sulfurylase Activities in groundnut (Arachis hypogea L.). J. Plant Biol. 49, 513–517 (2006). https://doi.org/10.1007/BF03031134

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03031134

Keywords

Search

Navigation