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Effect of growth regulators on asiaticoside production in whole plant cultures ofCentella asiatica (L) urban

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Abstract

We investigated the effects of growth regulators on whole-plant cultures derived from nodes ofCentella asiatica. A B5 liquid medium including 0.01 mg L-1 2,4-D resulted in decreased growth and asiaticoside production. Among the cytokinins tested (TDZ, BA, zeatin, and kinetin), TDZ was the best supplement for the promotion of asiaticoside biosynthesis. To directly estimate this effect, we measured asiaticoside content in the leaf, the main organ for synthesis. The addition of TDZ did not affect asiaticoside accumulation. Nevertheless, our results suggest that treatment with exogenous TDZ may enhance the production of asiaticoside in cultures simply through an increase in biomass.

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Authors and Affiliations

  1. Department of Biology, Chonnam National University, 500-757, Gwangju, Korea

    Ok-Tae Kim, Min-Young Kim, Sun-Mi Huh & Baik Hwang

  2. Department of Life Sciences, Seonam University, 590-170, Namwon, Korea

    Jun-Cheul Ahn

  3. National Institute of Crop Science, RDA, 441-857, Suwon, Korea

    Nak-Sul Seong

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  1. Ok-Tae Kim
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  2. Min-Young Kim
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  3. Sun-Mi Huh
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  4. Jun-Cheul Ahn
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  5. Nak-Sul Seong
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  6. Baik Hwang
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Correspondence to Baik Hwang.

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Kim, OT., Kim, MY., Huh, SM. et al. Effect of growth regulators on asiaticoside production in whole plant cultures ofCentella asiatica (L) urban. J. Plant Biol. 47, 361–365 (2004). https://doi.org/10.1007/BF03030552

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  • DOI: https://doi.org/10.1007/BF03030552

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