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A study of cotyledon senescence in cucumber (Cucumis sativus L.) based on expressed sequence tags and gene expression

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Abstract

Cucumber cotyledons provide an excellent experimental system in which to investigate developmental changes in gene expression, from the early phase of heterotrophism through phototrophic growth to senescence. A cDNA library was prepared from the final stage of senescing cucumber cotyledons (<95% yellow) for studying the genes responsible for lipid mobilization during germination and senescence. This library had produced numerous senescence-associated clones in a previous study. Here, a total of 365 cDNA clones and their expression levels were examined via semi-quantitative RT-PCR. Up-regulation of expression was detected for several known and unknown genes. These results were used to investigate the possible functions for senescence-related genes during cotyledon development

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  1. School of Science Education, College of Education, Chungbuk National University, 361-763, Chungbuk, Korea

    Dae -Jae Kim

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  1. Dae -Jae Kim
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Kim, D.J. A study of cotyledon senescence in cucumber (Cucumis sativus L.) based on expressed sequence tags and gene expression. J. Plant Biol. 47, 244–253 (2004). https://doi.org/10.1007/BF03030515

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  • DOI: https://doi.org/10.1007/BF03030515

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