Abstract
The feasibility of immunosuppression by corticosteroid hormones for the biocontrol of vertebrate pests was studied using water-soluble derivatives of prednisolone and dexamethasone administered by intubation to the levant vole (Microtus guentheri). Effect of treatment was determined through changes in body weight, histological changes in the spleen and bone marrow, and total and differential counts of white blood cells. Dexamethasone had a more drastic effect than prednisolone. In a group treated daily with 100 mg dexamethasone/kg body weight, signs of illness appeared in about 40% of the voles and death occurred in 18% of them 6–7 days after the start of the treatment. There was a loss of up to 22% in body weight, marked atrophy of the germinal center, corona and perifollicular zone of the splenic lymph nodules, but no effect on the bone marrow constitution. A similar treatment with prednisolone produced no external signs of illness or change in body weight, yet 17% of the voles died 8–10 days after the start of the treatment, and partial atrophy in the splenic lymph nodules occurred in 50% of the animals. In a group treated with 100 mg dexamethasone/kg once every 3 days, from which blood samples were taken repeatedly from the same eye, mortality was 100%, with death occurring 3–12 days after start of the treatment. Differential white blood cell counts indicated depletion of lymphocytes and monocytes, and increase in the number of granulocytes in the peripheral blood. Results of preference tests showed that prebaited voles do not reject wheat grain baits treated with a dexamethasone solution in water.
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References
Bach, J.F. (1975) The Mode of Action of Immunosuppressive Agents. North-Holland Publishing Co., Amsterdam.
Bach, J.F. (1976) The pharmacological and immunological basis for the use of immunosuppressive drugs.Drugs 11: 1–12.
Bodenheimer, F.S. (1949) Problems of the Vole Populations in the Middle East. Azriel Printing Co., Jerusalem.
Cohen, J.J. (1972) Thymus-derived lymphocytes sequestered on the bone marrow of hydrocortisone-treated mice.J. Immun. 108: 841–844.
Collins, F.M., Mackaness, G.B. and Blanden, R.V. (1966) Infection-immunity in experimental Salmonellosis.J. exp. Med. 124: 601–619.
Cooper, D.A., Duckett, M., Petts, V. and Penny, R. (1978) Corticosteroid enhancement of immunoglobulin synthesis by PWM stimulated human lymphocytes.Proc. 12th Int. Leucocyte Culture Conf. (Be’er Sheva’, Israel).
Davis, G.E. and Thompson, A. (1975) Effects of corticosteroid treatment and inflammation on the cellular content of blood and exudate in mice.J. Path. 115: 17–26.
Finelt, M. and Hoffmann, M.K. (1979) A human monocyte function test: Release of B-cell differentiation factor (BDF).Clin. Immun. Immunopath. 17: 281–288.
Haskell, G.M. (1977) Immunologic aspects of cancer chemotherapy.A. Rev. Pharmac. Toxic. 17: 179–195.
Heilman, D.H. and Leichner, J.P. (1972) Effect of Cortisol on the transformation of human blood lymphocytes by antigens and allogenic leucocytes,in: Schwarz, M.R. [Ed].Proc. 6th Int. Leucocyte Culture Conf. (New York, NY), p. 581. Academic Press, New York, NY.
Katz, D.H. and Benacerraf, B. (1972) The regulatory influence of activated T cells on B cell responses to antigen.Adv. Immun. 15: 1–94.
Marchalonis, J.J. (1978) Cell cooperation in immune response,in: Fundenberg, H.H., Stites, D.P., Caldwell, J.L. and Wells, J.V. [Eds]. Basic and Clinical Immunology, pp. 110–122. Lange Medical Publications, Los Altos, CA.
Olitzki, L. (1944) [Salmonella strains isolated in Palestine from different sources].Refuah Vet. 2: 1–8. (in Hebrew)
Sanderson, C.J. and De Souza, W. (1979) A morphological study of the interaction betweenTrypanosoma cruzi and rat eosinophiles, neutrophiles and macrophages.J. Cell Sci 37: 275–286.
Seed, J.R., Pinter, A.J., Ashman, P.U., Ackerman, S.A. and King, L. (1978) Comparison of organ weight of wild and laboratoryMicrotus montanus infected withTrypanosoma brucei gambiense. Am. Midl. Nat.100: 126–134.
Stavy, L., Cohen, I.R. and Feldman, M. (1973) The effect of hydrocortisone on lymphocytemediated cytolysis.Cell. Immun. 7: 302–312.
Stutman, O. (1977) Two main features of T-cell development: Thymus traffic and postthymic maturation,in: Stutman, O. [Ed]. Contemporary Topics in Immunology. Vol.7, pp. 1–46. Plenum Press, New York, NY.
Tompkins, W.A.F., Jarling, J.M. and Rawls, W.E. (1970)In vitro assessment of cellular immunity to vaccinia virus: contribution of lymphocytes and macrophages.Infection Immun. 2: 783.
Williams, R.T., Dunsmore, J.T. and Parer, I. (1972) Evidence for the latent myxoma virus in rabbits (Oryctolagus cuniculus L.).Nature, Lond. 238: 99–101.
Wodzicki, K. (1973) Prospects for biocontrol of rodent populations.Bull. Wld Hlth Orgn 48: 461–467.
Wolf, Y. (1977) The levant vole,Microtus guentheri (Danford & Alston 1882). Economic importance and control.EPPO Bull. 7: 277–281.
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Benjamini, L. The potential use of corticosteroid hormones in rodent biocontrol. Phytoparasitica 10, 215–228 (1982). https://doi.org/10.1007/BF03023962
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DOI: https://doi.org/10.1007/BF03023962