Abstract
Oxygen-derived free radicals produced during reperfusion may be responsible for the disturbed pathology which follows prolonged ischaemia. Measurement of hepatic chemilumine-scence (low level light emission resulting from the energy released during chemical reactions of free radicals) allowed determination of whether allopurinol could prevent formation of oxygen-derived free radicals during reperfusion of the ischaemic liver. While control animals demonstrated a burst of light emission shortly after reperfusion, the rats pretreated with allupurinol showed no evidence of chemiluminescence during either ischaemia or reperfusion. It is concluded that allopurinol may modify reperfusion-induced free radical formation and possibly ameliorate the organ damage which can follow ischaemia.
Résumé
Les radicaux libres dérivés de l’oxygène générés pendant la reperfusion peuvent être responsables des lésions pathologiques qui suivent l’ischémie prolongée. La mesure de la chimiolu-minescence (émission de lumière de faible intensité qui accompagne la libération d’énergie pendant les réactions chimiques propres à la formation des radicaux libres) a permis de déterminer si l’allopurinol pouvait prévenir la formation de radicaux libres dérivés de l’oxygène pendant la reperfusion du foie ischémique. Alors que les contrôles ont montré un jet d’émission lumineuse immédiatement après la reperfusion, les rats prétraités à l’allopurinol n’ont pas présenté de chimio-luminescence que ce soit pendant l’ischémie ou la reperfusion. On en conclut que l’allopurinol peut modifier la formation de radicaux libres dérivés de l’oxygène induite par la reperfusion et prévenir partiellement le dommage hépatique consécutif à l’ischémie.
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This investigation was supported in part by NIH Grant GM 29628 and University of Pennsylvania Biomedical Research Support Grant S07-RR-05415-27.
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Cohen, P.J. Allopurinol administered prior to hepatic ischaemia in the rat prevents chemiluminescence following restoration of circulation. Can J Anaesth 39, 1090–1093 (1992). https://doi.org/10.1007/BF03008379
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DOI: https://doi.org/10.1007/BF03008379