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Anti-inflammatory mechanisms of apigenin: inhibition of cyclooxygenase-2 expression, adhesion of monocytes to human umbilical vein endothelial cells, and expression of cellular adhesion molecules

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Abstract

The aim of this study was to clarify the anti-inflammatory mechanism of apigenin. Apigenin inhibited the collagenase activity involved in rheumatoid arthritis (RA) and suppressed lipopolysaccharide (LPS)-induced nitric oxide (NO) production in a dose dependent manner in RAW 264.7 macrophage cells. Pretreatment with apigenin also attenuated LPS-induced cyclooxygenase-2 (COX-2) expression. In addition, apigenin profoundly reduced the tumor necrosis factor-a (TNF-a)-induced adhesion of monocytes to HUVEC monolayer. Apigenin significantly suppressed the TNF-a-stimulated upregulation of vascular cellular adhesion mole-cule-1 (VCAM-1)-, intracellular adhesion molecule-1 (ICAM-1)-, and E-selectin-mRNA to the basal levels. Taken together, these results suggest that apigenin has significant anti-inflammatory activity that involves blocking NO-mediated COX-2 expression and monocyte adherence. These results further suggest that apigenin may be useful for therapeutic management of inflammatory diseases.

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References

  • Afanas’ev, I. B., Dorozhko, A. I., Brodskii, A. V, Kostyuk, A. V, and Potapovich, A. I., Chelating and free radical scavenging mechanisms of inhibitory action of rutin and quercetin in lipid peroxidation.Biochem. Pharmacol., 38, 1763–1769 (1989).

    Article  PubMed  CAS  Google Scholar 

  • Ahn, S. M., Yoon, H. Y, Lee, B. G, Park, K. C, Chung, J. H., Moon, C. H., and Lee, S. H., Fructose-1,6-diphosphate attenuates prostaglandin E2 production and Cyclo-oxigenase-2 expression in UVB-irradiated HaCaT keratinocytes.Br. J. Pharmacol., 137, 497–503 (2002).

    Article  PubMed  CAS  Google Scholar 

  • Aigner, T. and Stöve, J., Collagens- major component of the physiological cartilage matrix, major target of cartilage degeneration, major tool in cartilage repair.Adv. Drug Deliv. Rev., 55, 1569–1593 (2003).

    Article  PubMed  CAS  Google Scholar 

  • Amarowicz, R., Troszynska, A., and Shahidi, F., Antioxidant activity of armond seed extract and its fractions.J. Food Lipids, 12, 344–358 (2005).

    Article  CAS  Google Scholar 

  • Amrani, Y, Chen, H., and Panettieri, R., Activation of tumor necrosis factor receptor 1 in airway smooth muscle: A potential pathway that modulates bronchial hyper-responsiveness in asthma?Respir. Res., 1, 49–53 (2000).

    Article  PubMed  CAS  Google Scholar 

  • Antonella, S., Mario, S., Maria, L., Daniela, M., Francesco, B., and Francesco, C, Flavonoids as antioxidant agents: importance of their interaction with biomembrane.Free Rad. Biol. Med., 19, 481–486 (1995).

    Article  Google Scholar 

  • Arts, I. C. W. and Hollenberg, P. C. H., Polyphenols and disease risk in epidemiologic studies.Am. J. Clin. Nutr, 81, 317S- 325S (2005).

    PubMed  CAS  Google Scholar 

  • Baumann, J., Bruchhausen, F. V, and Wurm, G., Flavonoids and related compounds as inhibitors of arachidonic acid peroxidation.Prostaglandins, 20, 627–639 (1980).

    Article  PubMed  CAS  Google Scholar 

  • Belmont, H. M., Levartovsky, D., Goel, A., Amin, A., Giorno, R., Rediske, J., Skovron, M. L., and Abramson, S. B., Increased nitric oxide production accompanied by the up-regulation of inducible nitric oxide synthase in vascular endothelium from patients with systemic lupus erythematosus.Arthritis Rheum., 40, 1810–1816 (1997).

    Article  PubMed  CAS  Google Scholar 

  • Broide, D. H., Lotz, M., Cuomo, A. J., Coburn, D. A., Federman, E. C, and Wasserman, S. I., Cytokines in symptomatic asthma airway.J. Allergy Clin. Immunol., 89, 958–967 (1992).

    Article  PubMed  CAS  Google Scholar 

  • Chattopadhyay, S., Bhaumik, S., Purkayastha, M., Basu, S., Chaudhuri, A. N., and Gupta, S. D., Apoptosis and necrosis in developing brain cells due to arsenic toxicity and protection with antioxidants.Toxicol. Lett., 136, 65–76 (2002).

    Article  PubMed  CAS  Google Scholar 

  • Choi, J. S., Choi, Y. J., Park, S. H., Kang, J. S., and Kang, Y H., Flavones migrate tumor necrosis factor-a-induced adhesion molecule upregulation in cultured human endothelial cells: role of nuclear factor-KB.J. Nutr., 134, 1013–1019 (2004).

    PubMed  CAS  Google Scholar 

  • Chun, K. S., Cha, H. H., Shin, J. W., Na, H. K., Park, K. K., Chung, W. Y, and Surh Y J., Nitric oxide induces expression of cyclooxygenase-2 in mouse skin through activation of NFkB.Carcinogenesis, 25, 445–445 (2004)

    Article  PubMed  CAS  Google Scholar 

  • Corpe, C. P., Lee, J. H., Kwon, O., Eck, P., Narayanan, J., Kirk, K. L., and Levine, M., 6-Bromo-6-deoxy-L-ascorbic acid: an ascorbate analog specific for Na+-dependent vitamin C transporter but not glucose transporter pathways.J. Biol. Chem., 280, 5211–5220 (2005).

    Article  PubMed  CAS  Google Scholar 

  • Crofford, L. J., COX-1 and COX-2 tissue expression: Implication and predictions.J. Rheumatol., 24, 15–19 (1997).

    Google Scholar 

  • Dawson, T. M. and Dawson, V. L, Nitric oxide: actions and pathological roles.Neuroscientist, 1, 7–18 (1995).

    Article  CAS  Google Scholar 

  • Dubois, R. N., Abramson, S. B., Crofford, L, Gupta, R. A., Simon, L. S., Van De Putte, L. B., and Lipsky, P. E., Cyclo-oxygenase in biology and disease.FASEB J., 12, 1063–1073 (1998).

    PubMed  CAS  Google Scholar 

  • Edelstam, G A., Laurent, U. B., Lundkvist, O. E., Fraser, J. R., and Laurent, T. C, Concentration and turnover of intraperitoneal hyaluronan during inflammation.Inflammation, 16, 459–469 (1992).

    Article  PubMed  CAS  Google Scholar 

  • Elliott, M. J. and Kandaswami, C, The impact of plant flavonoids on mammalian biology: implications for immunity, inflammation and cancer. In Harborne, J. B. (Eds.). The Flavonoids: Advances in Research Since 1986. Chapman and Hall, London, pp. 619–651, (1986).

    Google Scholar 

  • Fischer, S. M. and Adam, L. M., Suppression of tumor promoter-induced chemiluminescence in mouse epidermal cells by several inhibitors of arachidonic acid metabolism.Cancer Res., 45, 3130–3136 (1985).

    PubMed  CAS  Google Scholar 

  • Fisher, N. D. L. and Hollenberg, N. K., Flavonoids for vascular health: the science behind the sweetness.J. Hypertens., 23, 1453–1459 (2005).

    Article  PubMed  CAS  Google Scholar 

  • Goldenberg, M. M., Celecoxib, a selective cyclooxygenase-2 inhibitor for the treatment of rheumatoid arthritis and osteoarthritis.Clin. Ther, 21, 1497–1513(1999).

    Article  PubMed  CAS  Google Scholar 

  • Gupta, S., Farrukh, A., and Hasan, M., Selective growth inhibitory, cell-cycle deregulatory and apoptotic response of apigenin in normal versus human prostate carcinoma cells.Biochem. Biophys. Res. Commun., 287, 914–920 (2001).

    Article  PubMed  CAS  Google Scholar 

  • Hirano, T., Oka, K., and Akiba, M., Anti-proliferative effects of synthetic and naturally occurring flavonoids on tumor cells of the human breast-carcinoma cell line, ZR-75-1. Res.Commun. Chem. Pathol. Pharmacol., 64, 69–78 (1989).

    CAS  Google Scholar 

  • Honda, S., Migita, K., Hirai, Y, Ueki, Y, Yamasaki, S., Urayama, S., Kawabe, Y, Fukuda, T., Kawakami, A., Kamachi, M., Kita, M., Ida, H., Aoyagi, T., and Eguchi, K., Induction of COX-2 expression by nitric oxide in rheumatoid synovial cells.Biochem. Biophysic. Res. Commun., 268, 928–931 (2000).

    Article  CAS  Google Scholar 

  • Huang, M. T., Lysz, T., Ferraro, T., Abidi, T. F, Laskin, J. D., and Conney, A. N., Inhibitory effects of curcumin onin vitro lipooxygenase and cyclooxygenase activities in mouse epidermis.Cancer Res., 51, 813–819 (1991).

    PubMed  CAS  Google Scholar 

  • Johansson, N., Saarialho-Kere, U., Airola, K., Herva, R., Nissinen, L, Westermarck, J., Vuorio, E., Heino, J., and Kähäri, V.-M., Collagenase-3 (MMP-3) is expressed by hypertrophic chondrocytes, periostreal cells, and osteoblasts during human fetal bone development.Dev. Dyn., 208, 387–397 (1997).

    Article  PubMed  CAS  Google Scholar 

  • Joyeux, M., Lobstein, A., Anton, R., and Mortier, F, Comparative anti-lipoperoxidant; antinecotic and scavenging properties of terpenes and biflavones from Ginkgo and some flavonoids.Planta Med., 61, 126–129 (1995).

    Article  PubMed  CAS  Google Scholar 

  • Kato, R., Nakadate, T., Yamamoto, S., and Sugimura, T., Inhibition of 12-O-tetradecacanolyphorbol 13-acetate-induced tumor promotion and ornithine decarboxylase activity by quercetin: possible involvement of lipoxugenesis inhibition.Carcino- genesis, 4, 1301–1305 (1983).

    Article  CAS  Google Scholar 

  • Kelm, M. A., Nair, M. G, Strasburg, G M., and De Witt, D. I., Antioxidant and cyclooxygenase inhibitory phenolic compounds fromOcimum sanctum.Linn. Phytomedicine, 7, 7–13 (2000).

    CAS  Google Scholar 

  • Koskinen, P. K. and Lemstrom, K. B., Adhesion molecule P- selectin and vascular cell adhesion molecule-1 in enhanced heart allograft arteriosclerosis in the rat.Circulation, 95, 191- 196 (1997).

    PubMed  Google Scholar 

  • Lee, C. W., Lin, W. N., Lin, C. C, Luo, S. F., Wang, J. S., Pouyssegur, J., and Yang, C. M., Transcriptional regulation of VCAM-1 expression by tumor necrosis factor-α in human tracheal smooth muscle cells: involvement of MAPKs, NF- kB, p300, and histone acetylation.J. Cell. Physiol., 207, 174- 186 (2006).

    Article  PubMed  CAS  Google Scholar 

  • Lee, J., Lee, S. H., Min, K. R., Lee, K. S., Ro, J. S., Ryu, J. C, and Kim, Y, Inhibitory effects of hydrolysable tannins on Ca2-activated HAase.Planta. Med., 59, 381–382 (1993).

    Article  PubMed  CAS  Google Scholar 

  • Lee, S. H., Soyoola, E., Chanmugam, P., Hart, S., Sun, W., Zhong, H., Liou, S., Simmons, D., and Hwang, D., Selective expression of mitogen-inducible cyclooxygenase in macrophages stimulated with lipopolysaccharide.J. Biol. Chem., 267, 25934–25938 (1992).

    PubMed  CAS  Google Scholar 

  • Lee, Y M., Choi, S. I., Lee, J. W., Jung, S. M., Park, S. M., and Heo, T. R., Isolation of hyaluronidase inhibitory component from the roots ofAstraglus membranaceus Bunge (Astragali Radix).Food Sci. Biotechnol., 14, 263–267 (2005).

    CAS  Google Scholar 

  • Lepley, D. M. and Pelling, J. C, Induction of p21/WAF1 and G1 cell cycle arrest by the chemopreventive agent apigenin.Mol. Carcinog., 19, 74–82 (1997).

    Article  PubMed  CAS  Google Scholar 

  • Liao, X., Lui, J. M., Du, L., Tang, A., Shang, Y, Wang, S. Q., Chen, L. Y, and Chen, Q., Nitric oxide signaling in stretchinduced apoptosis of neonatal rat cardiomyocytes.FASEB J., 20, E1196-E1204 (2006).

    Article  CAS  Google Scholar 

  • Lin, J. K., Chen, Y C, Huang, Y T., and Lin-Shiau, S. Y, Suppression of protein kinase C and nuclear oncogene expression as possible molecular mechanisms of cancer chemoprevention by apigenin and curcumin.J. Cell. Biochem. Suppl., 28–29, 39–48 (1997).

    Article  PubMed  Google Scholar 

  • Ludwig, A., Lorenz, M., Grimbo, N., Steinle, F., Meiners, S., Bartsch, C, Stangl, K., Baumann, G, and Stangl, V., The tea flavonoid epigallocatechin-3-gallate reduces cytokine-induced VCAM-1 expression and monocyte adhesion to endothelia cells.Biochem. Biophys. Res. Commun., 316, 659–665 (2004).

    Article  PubMed  CAS  Google Scholar 

  • Maksimowicz-McKinnon, K., Bhatt, D. L., and Calabrese, L. H., Recent advances in vascular inflammation: C-reactive protein and other inflammatory biomarkers.Curr. Opin. Rheumatol., 16, 18–24 (2004).

    Article  PubMed  CAS  Google Scholar 

  • Maruta, A., Ishei, T, and Uyeta, M., Mutagenicity of quercetin and kaempferol on cultured mammarian cells.Gann., 70, 273–276 (1982).

    Google Scholar 

  • Mayer, K., Merfels, M., Muhly-Reinholz, M., Gokorsch, S., Rosseau, S., Lohmeyer, J., Schwarzer, N., Krüll, M., Suttorp, N., Grimminger, F., and Seeger, W., w-3 Fatty acids suppress monocyte adhesion to human endothelial cells: role of endothelial RAF generation.Am. J. Physiol. Heart Circ. Physiol., 283, H811-H818 (2002).

    PubMed  CAS  Google Scholar 

  • McPhail, D. B., Hartley, R. C, Gardner, P. T, and Duthie, G G, Kinetic and stoichiometric assessment of the antioxidant activity of flavonoids by electron spin resonance spectroscopy.J. Agric. Food Chem., 51, 1684–1690 (2003).

    Article  PubMed  CAS  Google Scholar 

  • Moosmann, B. and Behl, C, The antioxidant neuroprotective effects of estrogens and phenolic compounds are independent from their estrogenic properties.Biochemistry, 96, 8867- 8872 (1999).

    CAS  Google Scholar 

  • Morel, I., Lescoat, G, Cogrel, P., Sergent, O., Pasdeloup, N., Brissot, P., Cillard, P., and Cillard, J., Antioxidants and ironchelating activities of the flavonoids catechin, quercetin and diosmetin on iron-loaded rat hepatocyte culture.Biochem. Pharmacol., 45, 13–19 (1993).

    Article  PubMed  CAS  Google Scholar 

  • Nielsen, S. E., Young, J. F, Daneshvar, B., Lauridsen, S. T, Knuthsen, P. S., Strom, B., and Dragster, L. O., Effect of parsley (pertroselinum crispum) intake on urinary apigenin excretion blood antioxidant enzymes and bio-makers for oxidative stress in human subjects.Br. J. Nutr., 81, 425–426 (1999).

    Google Scholar 

  • Noble, P. W., Mackee, C. M., Coweman, M., and Shin, H. S., Hyaluronan fragments activate an NF-kappa B/l-kappa B alpha autoregulatory loop in murine macrophages.J. Exp. Med, 183, 2373–2378 (1996).

    Article  PubMed  CAS  Google Scholar 

  • Nuutila, A. M., Puupponen-Pimiä, R., Aarni, M., and Oksman-Caldentey, K.-M., Comparison of antioxidant activities of onion and garlic extracts by inhibition of lipid peroxidation and radical scavenging activity.Food Chem., 81, 485–493 (2003).

    Article  CAS  Google Scholar 

  • Oyaizu, M., Studies on product of browning reaction: antioxidative activities of products of browning reaction prepared from glucosamine.Jpn. J. Nutr., 44, 307–315 (1986).

    CAS  Google Scholar 

  • Park, H,, Sin, B. Y, and Kim, H. P., Inhibition of collagenase by anti-inflammatory synthetic flavones.J. Appl. Pharmacol., 14, 36–39 (2006).

    CAS  Google Scholar 

  • Park, S.-H., Park, J. H. Y, Kang, J. S., and Kang, Y H., Involvement of transcription factors in plasma HDL protection against TNF-a-induced vascular cell adhesion molecule-1 expression.Int. J. Biochem. Cell Biol., 35, 168–182 (2003).

    Article  PubMed  CAS  Google Scholar 

  • Psotovâ, J., Chlopèiková, Š., Miketová, P., Hrbáè, J., and Šimánek, V, Chemoprotective effect of plant phenolics against anthracycline-induced toxicity on rat cardiomyocytes. Part III. Apigenin, bacalein, kaempferol, leuteolin and quercetin.Phytother. Res., 18, 516–521 (2004).

    Article  PubMed  CAS  Google Scholar 

  • Rairigh, R. L, Le Cras, T. D., Ivy, D. D., Kinsella, J. P., Richter, G, Horan, M. P., Fan, I. D., and Abman, S. H., Role of inducible nitric oxide synthase in regulation of pulmonary vascular tone in the late gestation ovine fetus.J. Clin. Invest, 101, 15–21 (1998).

    Article  PubMed  CAS  Google Scholar 

  • Safari, M. R. and Sheikh, N., Effects of flavonoids on the susceptibility of low-density lipoprotein to oxidative modification.Prostaglandin Leukot Essent Fatty Acids, 69, 73–77 (2003).

    Article  CAS  Google Scholar 

  • Sawabe, Y, Nakagomi, K., Iwagami, S., Suzuki, S., and Nakazawa, H., Inhibitory effects of pectic substances on activated hyaluronidase and histamine release from mast cells.Biochim. Biophys. Acta, 1137, 274–278 (1992).

    Article  PubMed  CAS  Google Scholar 

  • Sawabe, Y, Yamasaki, K., Iwagami, S., Kazimura, K., and Nakagommi, K., Inhibitory effects of natural medicines on the enzymes related to the skin.Yakugaku Zasshi, 118, 423–429 (1998).

    PubMed  CAS  Google Scholar 

  • Schauss, A. G, Merkel, D. J., Glaza, S. M., and Sorenson, S. R., Acute and subchronic oral toxicity studies in rats of a hydrolyzed chicken sternal cartilage preparation.Food Chem. Toxicol., 45, 315–321 (2007).

    Article  PubMed  CAS  Google Scholar 

  • Silverman, M. D., Zamora, D. O., Pan, Y, Texeira, P. V, Planck, S. R., and Rosenbaum, J.T., Cell adhesion molecule expression in cultured human iris endothelial cells.Invest. Ophthalmol. Vis. Sci., 42, 2861–2866 (2001).

    PubMed  CAS  Google Scholar 

  • Sin, B. Y and Kim, H. P., Inhibition of collagenase by naturallyoccurring flavonoids.Arch. Pharm. Res., 28, 1152–1155 (2005).

    Article  PubMed  CAS  Google Scholar 

  • Singh, J. P., Selvendiran, K., Banu, M. S., Padmavathi, R., and Sakthisekaran, D., Protective role of apigenin on the status of lipid peroxidation and antioxidant defense against hepatocarcinogenesis in Wistar albino rats.Phytomedicine, 11, 309- 314 (2004).

    Article  PubMed  CAS  Google Scholar 

  • Stenman, M., Ainola, M., Valmu, L, Bjartell, A., Ma, G, Stenman, U. H., Sorsa, T., Luukkainen, R., and Konttinen, Y T., Trypsin-2 degrades human type II collagen and is expressed and activated in mesemchymally transformed rheumatoid arthritis synovitis tussue.Am. J. Pathol., 167, 1119–1124 (2005).

    PubMed  CAS  Google Scholar 

  • Stoewsand, G S., Anderson, J. L., Boyd, J. N., Hrazdina, G, Babish, J. G, Walsh, K. M., and Losco, P., Quercetin: a mutagen, not a carcinogen, in Fisher rats.J. Toxicol. Environ. Hlth., 14, 105–114 (1984).

    Article  CAS  Google Scholar 

  • Tchetina, E. V., Kobayashi, M., Yasuda, T., Meigers, T., Pidoux, I., and Roole, A. R., Chondrocyte hypertrophy can be induced by a cryptic sequence of type II collagen and is accompanied by in induction of MMP-13 and collagenase activity: implication for development and arthritis.Matrix Biol., 26, 247–258 (2007).

    Article  PubMed  CAS  Google Scholar 

  • Van Acker, S. A., van den Berg, D. J., Tramp, M. N., Griffioen, D. H., van Bennekom, W. P., van der Vijgh, W. J., and Bast, A., Structural aspects of antioxidant activity of flavonoids.Free Rad Biol. Med, 20, 331–342 (1996).

    Article  PubMed  Google Scholar 

  • Van Dross, R. T., Hong, X., and Pelling, J. C, Inhibition of TPA- induced cyclooxygenase-2 (COX-2) expression by apigenin through downregulation of Akt signal transduction in human keratinocytes.Mol. Carcinog., 44, 83–91 (2005).

    Article  PubMed  CAS  Google Scholar 

  • Vita, J. A., Polyphenols and cardiovascular disease: effects on endothelial and platelet function.Am. J. Clin. Nutr., 81, 292S- 297S (2005).

    PubMed  CAS  Google Scholar 

  • Waddington, E., Puddey, I. B., and Croft, K. D., Red wine poly- phenolic compounds inhibit atherosclerosis in apolipoprotein E-deficient mice independent of effects on lipid peroxidation.Am. J. Clin. Nutr, 79, 54–61 (2004).

    PubMed  CAS  Google Scholar 

  • Wei, H., Tye, L., Bresnick, E., and Birt, D. F., Inhibitory effect of apigenin, a plant flavonoid, an epidermal ornithine decarb- oxylase and skin-tumor promotion in mice.Cancer Res., 50, 499–502 (1990).

    PubMed  CAS  Google Scholar 

  • Xie, W. L, Chipman, J. G, Robertson, D. L, Erikson, R. L., and Simmons, D. L., Expression of a mitogen-responsive gene encoding prostaglandin synthase is regulated by mRNA splicing.Proc. Natl. Acad. Sci. U.S.A., 88, 2692–2696 (1991).

    Article  PubMed  CAS  Google Scholar 

  • Yen, G-C. and Chen, H.-Y, Antioxidant activity of various tea extracts in relation to their antimutagenicity.J. Agric. Food Chem., 43, 27–32 (1995).

    Article  CAS  Google Scholar 

  • Yukiko, H., Shunjiro, O., and Shozo, F., The correlation between active oxygens scavenging and antioxidative effects of flavonoid.Free Rad. Biol. Med., 16, 845–850 (1994).

    Article  Google Scholar 

  • Zimmerman, G A., Mclntyre, T. M., and Prescott, S. M., Adhesion and signaling in vascular cell-cell interactions.J. Clin. Invest, 100, S3-S5 (1997).

    PubMed  CAS  Google Scholar 

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  1. Plant Resources Research Institute, Duksung Women’s University, 132-714, Seoul, Korea

    Je-Hyuk Lee

  2. National Products Research Institute, College of Pharmacy, Seoul National University, 110-460, Seoul, Korea

    Hong Yu Zhou, So Yean Cho & Yeong Shik Kim

  3. College of Pharmacy, Duksung Women’s University, 419 Ssangmun, Dobong-Gu, 132-714, Seoul, Korea

    Yong Soo Lee & Choon Sik Jeong

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  1. Je-Hyuk Lee
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Lee, JH., Zhou, H.Y., Cho, S.Y. et al. Anti-inflammatory mechanisms of apigenin: inhibition of cyclooxygenase-2 expression, adhesion of monocytes to human umbilical vein endothelial cells, and expression of cellular adhesion molecules. Arch. Pharm. Res. 30, 1318–1327 (2007). https://doi.org/10.1007/BF02980273

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