Abstract
The mutagenicity of the mycotoxin patulin was assessed by the thymidine kinase mutation assay, which is based on point mutations and deletions. Patulin was mutagenic in cultured mouse lymphoma cells and the mutagenicity was significantly increased in cells pretreated with buthionine sulfoximine, which reduces intracellular glutathione levels.
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Pfeiffer E, Gross K, Metzler M (1998) Aneuploidogenic and clastogenic potential of the mycotoxins citrinin and patulin. Carcinogenesis 19: 1313–1318
Schumacher D, Metzler M, Lehmann L. Mutagenicity of the mycotoxin patulin in cultured Chinese hamster V79 cells, and its modulation by intracellular glutathione. Arch Toxicol. In press (DOI: 10.1007/s00204-004-0612-x)
Fliege R, Metzler M (2000) Electrophilic properties of patulin. N-acetylcysteine and glutathione adducts. Chem Res Toxicol 13: 373–381
Fliege R, Metzler M (2000) Electrophilic properties of patulin. Adduct structures and reaction pathways with 4-bromothiophenol and other model nucleophiles. Chem Res Toxicol 13: 363–372
Clements J (2000) The mouse lymphoma assay. Mutat Res 455: 97–110
Cook JA, Mitchell JB (1995) Measurement of thiols in cell populations from tumor and normal tissue. Methods Enzymol 251: 203–212
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Financial support Deutsche Forschungsgemeinschaft (Grant Me 574/14-2)
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Schumacher, D.M., Wagner, J., Metzler, M. et al. Influence of decreased intracellular glutathione level on the mutagenicity of patulin in cultured mouse lymphoma cells. Mycotox Res 21, 150–152 (2005). https://doi.org/10.1007/BF02954442
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DOI: https://doi.org/10.1007/BF02954442