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A contribution to the quantitative evaluation of yeast cultures stored in liquid nitrogen

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Abstract

Different ways of the evaluation of viability of cultures stored in liquid nitrogen are compared. The streaking technique is recommended for a rapid evaluation. It is sufficient to determine the suitability of the medium, of cryopreservatives and of the whole procedure. On storage of melted cultures for 1–7 d at 4 °C it was found that the cultures could be transported directly in a suitable package maintaining the low temperature on the condition that the transportation would not last longer than 5 d.

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Authors and Affiliations

  1. Chemical Institute, Chemical Research Centre, Slovak Academy of Sciences, 842 38, Bratislava, Czechoslovakia

    A. Kocková-Kratochvílová & R. Kovačovská

Authors
  1. A. Kocková-Kratochvílová
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  2. R. Kovačovská
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Additional information

The authors wish to thank Mrs. Milena Jurěová for her excellent technical assistance.

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Kocková-Kratochvílová, A., Kovačovská, R. A contribution to the quantitative evaluation of yeast cultures stored in liquid nitrogen. Folia Microbiol. 31, 367–374 (1986). https://doi.org/10.1007/BF02936601

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  • DOI: https://doi.org/10.1007/BF02936601

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