Abstract
The degradation of 4-chlorobiphenyl (4CB) was compared in field lysimeters containing 60 Kg of soil contaminated with 5–10 mg/Kg of polychlorinated biphenyls.Alcaligenes A5, a bacterium carrying a plasmid for 4CB degradation, was inoculated into three lysimeters. When compared to an untreated control, soil samples from water, mineral, and yeast extract treated lysimeters with and without a bacterial inoculum exhibited greater than 10-fold increases in the rate of [1-14 C-acetate incorporation into lipids and14CO2 production from [U-14C-4-chlorobiphenyl. Gene probe analyses for the 4CB plasmid and most-probable-number enumerations demonstrated the presence of biodegradative populations in lysimeters and the probable survival of the addedAlcaligenes A5.
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Hill, D.L., Phelps, T.J., Palumbo, A.V. et al. Bioremediation of polychlorinated biphenyls degradation capabilities in field lysimeters. Appl Biochem Biotechnol 20, 233–243 (1989). https://doi.org/10.1007/BF02936485
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DOI: https://doi.org/10.1007/BF02936485