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Application of a compatible xylose isomerase in simultaneous bioconversion of glucose and xylose to ethanol

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Abstract

Simultaneous isomerisation and fermentation (SIF) of xylose and simultaneous isomerisation and cofermentation (SICF) of glucose-xylose mixture was carried out by the yeastSaccharomyces cerevisiae in the presence of a compatible xylose isomerase. The enzyme converted xylose to xylulose andS. cerevisiae fermented xylulose, along with glucose, to ethanol at pH 5.0 and 30°C. This compatible xylose isomerase fromCandida boidinii, having an optimum pH and temperature range of 4.5–5.0 and 30–50°C respectively, was partially purified and immobilized on an inexpensive, inert and easily available support, hen egg shell. An immobilized xylose isomerase loading of 4.5 IU/(g initial xylose) was optimum for SIF of xylose as well as SICF of glucose-xylose mixture to ethanol byS. cerevisiae. The SICF of 30 g/L glucose and 70 g xylose/L gave an ethanol concentration of 22.3 g/L with yield of 0.36 g/(g sugar consumed) and xylose conversion efficiency of 42.8%.

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  1. Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Delhi, Hauz Khas, 110016, New Delhi, India

    Priya Chandrakant & Virendra S. Bisaria

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  1. Priya Chandrakant
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  2. Virendra S. Bisaria
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Correspondence to Virendra S. Bisaria.

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Chandrakant, P., Bisaria, V.S. Application of a compatible xylose isomerase in simultaneous bioconversion of glucose and xylose to ethanol. Biotechnol. Bioprocess Eng. 5, 32–39 (2000). https://doi.org/10.1007/BF02932350

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  • DOI: https://doi.org/10.1007/BF02932350

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