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Synthesis of polyrotaxane-biotin conjugates and surface plasmon resonance analysis of streptavidin recognition

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Abstract

A polyrotaxane-biotin conjugate was synthesized and its interaction with streptavidin measured using surface plasmon resonance (SPR) detection. A biodegradable polyrotaxane in whichca. 22 molecules of α-cyclodextrins (α-CDs) were threaded onto a poly(ethylene oxide) chain (M n: 4,000) capped with benzyloxycarbonyl-L-phenylalanine was conjugated with a biotin hydorazide and 2-aminoethanol after activating the hydroxyl groups of α-CDs in the polyrotaxane usingN,N′-carbony diimidazole. The results of the high-resolution1H-nuclear magnetic resonance (1H-NMR) spectra and gel permeation chromatography of the conjugate showed thatca. 11 biotin molecules were actually introduced to the polyrotaxane scaffold. An SPR analysis showed that the binding curves of the biotin molecules in the conjugate on the streptavidin-deposited surface changed in a concentration dependent manner, indicating that the biotin in the conjugate was actually recognized by streptavidin. The association equilibrium constant (K a) of the interaction between the conjugate and streptavidin tetramer was of the order 107. These results suggest that polyrotaxane is useful for scaffolds as a polymeric ligand in biomedical fields.

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Authors and Affiliations

  1. School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, 923-1292, Tatsunokuchi, Ishikawa, Japan

    Tooru Ooya, Tomokatsu Kawashima & Nobuhiko Yui

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  1. Tooru Ooya
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  2. Tomokatsu Kawashima
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  3. Nobuhiko Yui
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Correspondence to Nobuhiko Yui.

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Ooya, T., Kawashima, T. & Yui, N. Synthesis of polyrotaxane-biotin conjugates and surface plasmon resonance analysis of streptavidin recognition. Biotechnol. Bioprocess Eng. 6, 293–300 (2001). https://doi.org/10.1007/BF02931993

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