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Influence of benomyl on photosynthetic capacity in soybean leaves

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Abstract

This investigation was performed to study the influence of benomyl on photosynthetic pigments and enzymes in soybean leaves. Chlorophyll and pheophytin levels were reduced by benomyl 45 days after greening. These results indicate that chlorophylla andb, and pheophytin must be controlled by benomyl. SDS-PAGE analysis showed that 50 and 14.5 kD polypeptides represented as the large and small subunits of rubisco. In the both of these subunits, the band intensity of the control was significantly higher than that after benomyl treatment, indicating that these two subunits are affected by benomyl. Benomyl strongly inhibited both the activity and content of rubisco as its concentration was gradually increased. However, it remains unclear whether this reduction of rubisco level was due to a reduced level of rubisco activase. Two major polypeptides of 46 and 42 kD were identified as rubisco activase subunits by SDS-PAGE. The intensity of these two bands was shown to be higher in the control than after benomyl treatment. These results indicate that the rubisco decrease resulting from increased benomyl concentrations was caused by rubisco activase. A significant decrease in both the activity and content of rubisco activase by benomyl was also observed. These results suggest that the decrease in rubisco level caused by benomyl is accompanied by a decrease in both the activity and content of rubisco activase.

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Abbreviations

BTP:

Bis-tris propane

DMF:

N,N-Dimethylformamide

DTT:

Dithiothreitol

GSH:

Glutathione

MBT:

Mercaptobenzothiazole

PEG:

Polyethylene glycol

PMSF:

Phenylmethylsulfonylfluoride

PSII:

Photosystem II

PVPP:

Polyvinylpolypyrrolidone

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Authors and Affiliations

  1. Department of Biology, Keimyung University, 704-701, Daegu, Korea

    Kwang Soo Roh & Mi Jung Oh

  2. Department of Biology, Kyungpook National University, 702-701, Daegu, Korea

    Seung Dal Song

  3. Department of Biological Education, Kyungpook National University, 702-701, Daegu, Korea

    Hwa Sook Chung

  4. Department of Biology, Andong National University, 760-749, Andong, Korea

    Jong Suk Song

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  1. Kwang Soo Roh
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Correspondence to Kwang Soo Roh.

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Roh, K.S., Oh, M.J., Song, S.D. et al. Influence of benomyl on photosynthetic capacity in soybean leaves. Biotechnol. Bioprocess Eng. 6, 100–106 (2001). https://doi.org/10.1007/BF02931954

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