Abstract
A series of lactobacilli isolated from dairy products were characterized using biotyping and ribotyping withEcoRI andHindIII restriction enzymes. Biotyping assigned 14 strains asLactobacillus casei, 6 strains asLactobacillus paracasei subsp.paracasei and 12 asLactobacillus rhamnosus. The obtained ribotype patterns separated all analyzed strains into two clearly distinguished groups corresponding toL. rhamnosus andL. casei/L. paracasei subsp.paracasei. TheHindIII ribotypes of individual strains representing these two groups were visually very similar. In contrast,EcoRI ribotyping revealed high intraspecies variability. All ribotypes ofL. casei andL. paracasei subsp.paracasei dairy strains were very close and some strains even shared identical ribotype profiles. The type strainsL. casei CCM 7088T (= ATCC 393T) andLactobacillus zeae CCM 7069T revealing similar ribopatterns formed a separate subcluster using both restriction enzymes. In contrast, the ribotype profile ofL. casei CCM 7089 (= ATCC 334) was very close to ribopatterns obtained from the dairy strains. These results support synonymy ofL. casei andL. paracasei species revealed by other studies as well as reclassification of the type strainL. casei CCM 7088T asL. zeae and designation ofL. casei CCM 7089 as the neotype strain.
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The study was supported by the project of theMinistry of Agriculture of the Czech Republic no. QE 1382 priority III-D-01 and the project of theGrant Agency of the Czech Republic no. 204/02/D099.
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Švec, P., Dráb, V. & Sedláček, I. Ribotyping ofLactobacillus casei group strains isolated from dairy products. Folia Microbiol 50, 223–228 (2005). https://doi.org/10.1007/BF02931570
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DOI: https://doi.org/10.1007/BF02931570