Skip to main content
Log in

Recent studies of the hematopoietic microenvironment in long-term bone marrow cultures

  • Hematopoiesis, Symposium
  • Published:
Immunologic Research Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Greenberger JS, Aaronson SA: Selective techniques for isolation of morphologic revertants of Kirsten murine sarcome virus transformed non-produced BALB/3T3: biologic properties in vitro and in vivo. Virology 1974; 57: 339–346.

    Article  PubMed  CAS  Google Scholar 

  2. Greenberger JS: Sensitivity of corticosteroid-dependent, insulin-resistant lipogenesis in marrow preadipocytes of mutation diabetic-obese mice. Nature 1978; 275: 752–754.

    Article  PubMed  CAS  Google Scholar 

  3. Greenberger JS: Corticosteroid dependent differentiation of human bone marrow preadipocytes. In Vitro 1979; 15: 823–828.

    Article  PubMed  CAS  Google Scholar 

  4. Greenberger JS, Sakakeeny MA, Parker LM: In vitro proliferation of hematopoietic stem cells in long-term marrow cultures: principals in mouse applied to man. Exp Hematol 1979; (suppl 5): 135–148.

    Google Scholar 

  5. Mauch P, Greenberger JS, Botnick LE, et al: Evidence for structured variation in self-renewal capacity within long-term bone marrow cultures. Proc Natl Acad Sci USA 1980; 77: 2927–2930.

    Article  PubMed  CAS  Google Scholar 

  6. Greenberger JS: Self-renewal of factor-dependent hematopoietic progenitor cell lines derived from long-term bone marrow cultures demonstrates significant mouse strain genotypic variation. J Supramol Struct 1980; 13: 501–511.

    Article  PubMed  CAS  Google Scholar 

  7. Sakakeeny MA, Greenberger JS: Granulopoiesis longevity in continuous bone marrow cultures and factor-dependent cell line generation: significant variation among 28 inbred mouse strains and outbred stocks. JNCI 1982; 68: 305–317.

    PubMed  CAS  Google Scholar 

  8. Greenberger JS, Hoffman N, Lieberman M, et al: Pool size of pluripotential hematopoietic stem cells increased in continuous bone marrow culture by Friend spleen focus forming virus. JNCI 1983; 70: 323–331.

    PubMed  CAS  Google Scholar 

  9. Cohen GI, Greenberger JS, Canellos GP: Electron microscopic changes occurring in bone marrow stromal cells exposed to cytotoxic drugs in vitro, Symposium on scanning EM of bone marrow. SEM 1982; 1: 359–365.

    Google Scholar 

  10. Greenberger JS, Sakakeeny MA, Humphries KC, et al: Demonstration of permanent factor-dependent multipotential (erythroid/neutrophil/basophil) hematopoietic progenitor cell lines. Proc Natl Acad Sci USA 1983; 80: 2931–2935.

    Article  PubMed  CAS  Google Scholar 

  11. Shadduck R, Waheed A, Greenberger JS, et al: Production of colony stimulating factor in long-term marrow cultures. J Cell Physiol 1983; 114: 88–92.

    Article  PubMed  CAS  Google Scholar 

  12. Greenberger JS: Long-term hematopoietic cultures; in Golde D (ed): Methods in Hematology. vol 11, New York, Churchill Livingstone, 1984, pp 203–243.

    Google Scholar 

  13. Greenberger JS, Eckner RJ, Sakakeeny M, et al: Role of stromal and hematopoietic stem cells in Friend spleen focus forming virus effects in continuous bone marrow culture. Leukemia Res 1983; 7: 621–636.

    Article  CAS  Google Scholar 

  14. Greenberger JS, Sakakeeny MA, Davis LM, et al: Biologic properties of factor-independent nonadherent hematopoietic and adherent preadipocyte cell lines derived from continuous bone marrow cultures. Leukemia Res 1984; 8: 363–375.

    Article  CAS  Google Scholar 

  15. Greenberger JS, Klassen V, Kase K, et al: Effects of low dose rate irradiation on plateau-phase bone marrow stromal cells in vitro: demonstration of a new form of non-lethal physiologic alteration of support of hematopoietic stem cells. Int J Rad Oncol Biol Phys 1984; 10: 1027–1037.

    CAS  Google Scholar 

  16. Greenberger JS, Rothstein L, DeFabritiis P, et al: Effects of monoclonal antibody and complement treatment of human marrow on hematopoiesis in continuous bone marrow culture. Cancer Res 1985; 45: 758–767.

    PubMed  CAS  Google Scholar 

  17. Greenberger JS, Humphries RK, Messner H, et al: Molecularly cloned and expressed murine T-cell gene product with multipotential CSF properties is biologically similar to interleukin-3. Exp Hematol 1985; 13: 249–260.

    PubMed  CAS  Google Scholar 

  18. Rothstein L, Pierce JH, Aaronson SA, et al: Amphotropic retrovirus vector transfer of the v-ras oncogene into human hematopoietic and stromal cells in continuous bone marrow culture. Blood 1985; 65: 744–752.

    PubMed  CAS  Google Scholar 

  19. Greenberger JS, Palaszynski EW, Pierce JH, et al: Biologic effects of prolonged L-Phenylalanine mustard treatment of murine long-term bone marrow cultures and IL-3 dependent hematopoietic progenitor cell lines. JNCI 1985; 74: 247–262.

    PubMed  CAS  Google Scholar 

  20. Naparstek E, Donnelly T, Kase K, et al: Biologic effects of in vitro x-irradiation of murine long-term bone marrow cultures on the production of granulocyte-macrophage colony stimulating factors. Exp Hematol 1985; 13: 701–708.

    PubMed  CAS  Google Scholar 

  21. FitzGerald TJ, Rothstein L, Kase K, et al: Radio-sensitivity of human bone marrow granulocyte-macrophage progenitor cells: effect of dose rate on purified target cell populations. Rad Res 1986; 107:205–215.

    Article  CAS  Google Scholar 

  22. Naparstek E, Donnelly T, Shadduck R, et al: Persistent production of granulocyte-macrophage colony-stimulating factor (CSF-1) by cloned bone marrow stromal cell line D2XRII after x-irradiation. J Cell Physiol 1986; 126: 407–417.

    Article  PubMed  CAS  Google Scholar 

  23. Naparstek E, Pierce J, Metcalf D, et al: Induction of growth alterations in factor-dependent hematopoietic progenitor cell lines by cocultivation with irradiated bone marrow stromal cell lines. Blood 1986; 67: 1395–1403.

    PubMed  CAS  Google Scholar 

  24. FitzGerald TJ, Rothstein LA, Daugherty C, et al: The activated human n-ras oncogene enhances x-irradiation repair of mammalian cells in vitro less effectively at low dose rate: implications for increased therapeutic ration of low dose rate irradiation. Am J Clin Oncol 1985; 8: 517–522.

    Article  PubMed  CAS  Google Scholar 

  25. Greenberger JS, Key L, Daugherty C, et al: Establishment of bone marrow stromal cell cultures and permanent clonal cell lines from osteopetrotic (mi/mi) and Steel mutant (S1/S1d) mice: studies of bone resorption by engrafted hemopoietic stem cells in vitro; in Baum SJ (ed): Experimental Hematology Today. New York, Springer, 1986, pp 42–49.

    Google Scholar 

  26. Hardy CL, Kishimoto T, Harjes K, et al: Homing of a cloned multipotential stem cell line in hemopoietic tissues. Exp Hematol 1986; 14: 636–642.

    PubMed  CAS  Google Scholar 

  27. Naparstek E, FitzGerald TJ, Sakakeeny MA, et al: X-irradiation of bone marrow stromal cells in vitro induces malignant transformation of cocultivated hematopoietic stem cells. Cancer Res 1986; 46: 4677–4684.

    PubMed  CAS  Google Scholar 

  28. Greenberger JS: Future directions in clinical bone marrow transplantation: interests converge on the bone marrow microenvironment. Br J Hematol 1986; 62: 603–605.

    Article  CAS  Google Scholar 

  29. Greenberger JS, Daugherty C, Sakakeeny MA, et al: Friend virus-infected long-term bone marrow cultures produce colony stimulating factor-dependent and independent granulocytic-macrophage progenitor cells for over four years in vitro. Leukemia Res 1987; 11: 51–61.

    Article  CAS  Google Scholar 

  30. Naparstek E, Anklesaria P, FitzGerald TJ, et al: Morphology of cell-to-cell contact between x-irradiated plateau-phase bone marrow stromal cell lines and cocultivated factor-dependent cell lines leading to leukemogenesis in vitro. SEM 1987; 1: 247–253.

    CAS  Google Scholar 

  31. Anklesarie P, Klassen V, Sakakeeny MA, et al: Biologic characterization of cloned permanent stromal cell lines from anemic S1/S1d and +/+ littermates. Exp Hematol 1987; 15: 636–644.

    Google Scholar 

  32. Anklesaria P, Holland C, Klassen V, et al: Expression of a selectable gene transferred by retroviral vector to hematopoietic stem cells and stromal cells in murine continuous bone marrow cultures. Exp Hematol 1987; 15: 195–202.

    PubMed  CAS  Google Scholar 

  33. Greenberger JS, FitzGerald TJ, Klassen V, et al: Alteration in hematopoietic stem cell seeding and proliferation by low dose rate irradiation of bone marrow stromal cells in vitro. Int J Rad Oncol Biol Phys 1988; 14: 85–94.

    CAS  Google Scholar 

  34. Anklesaria P, Kase KR, Glowacki J, et al: Engraftment of a clonal bone marrow stromal cell line in vivo stimulates hematopoietic recovery from total body irradiation. Proc Natl Acad Sci USA 1987; 84: 7681–7685.

    Article  PubMed  CAS  Google Scholar 

  35. Ohta M, Massague J, Anklesaria P, et al: Type β transforming growth factor (TGB-β) regulates differentiation and proliferation of multipotential hematopoietic progenitor cells. Nature 1987; 329: 539–541.

    Article  PubMed  CAS  Google Scholar 

  36. Zipori D, Toledo J, von der Mark K: Phenotypic heterogeneity among stromal cell lines from mouse marrow disclosed in their extracellular matrix composition and interactions with normal and leukemia cells. Blood 1985; 66: 447–455.

    PubMed  CAS  Google Scholar 

  37. Ohta M, Anklesaria P, FitzGerald TJ, et al: Long-term bone marrow cultures: recent studies with clonal hematopoietic and stromal cell lines. Pathol Immunopathol Res 1989; 8: 1–20.

    Article  PubMed  CAS  Google Scholar 

  38. Bently A, Kirby L, Anklesaria P, et al: Hematopoietic stromal proteoglycan heterogeneity: phenotypic variability between cell lines and the effects of glucocorticoid. J Cell Physiol 1988; 136: 182–187.

    Article  Google Scholar 

  39. Pierce J, Ruggiero M, Fleming TP, et al: Signal transduction through the EGF receptor transfected into interleukin-3-dependent hematopoietic cells. Science 1985; 239: 628–631.

    Article  Google Scholar 

  40. FitzGerald TJ, Santucci MA, Harigaya K, et al: Radiosensitivity of permanent human bone marrow stromal cell lines: effect of dose rate. Int J Rad Oncol Biol Phys. in press.

  41. FitzGerald TJ, Anklesaria P, Le D, et al: Radiosensitivity of cloned permanent murine bone marrow stromal cell lines: non-uniform effect of low dose rate. Exp Hematol, in press.

  42. Whitlock CA, Robertson D, Witte ON: Murine B-cell lymphopoiesis in long term culture. J Immunol Methods 1984;67:353–369.

    Article  PubMed  CAS  Google Scholar 

  43. Zipori D, Tamir M, Toledo J, et al: Differentiation stage and lineage-specific inhibitor from the stroma of mouse bone marrow that restricts lymphoma cell growth. Proc Natl Acad Sci USA 1986; 83:4547–4551.

    Article  PubMed  CAS  Google Scholar 

  44. Singer J, Charbord P, Keating A, et al: Simian virus 40-transformed adherent cells from human long-term marrow cultures. Cloned cell lines produce cells with stromal and hematopoietic characteristics. Blood 1987;70:464–474.

    PubMed  CAS  Google Scholar 

  45. Whitlock CA, Tidmarsh GF, Muller-Sieburg C, et al: Bone marrow stromal cell lines with lymphopoietic activity express high levels of a pre-B neoplasia-associated molecule. Cell 1987;48:1009–1021.

    Article  PubMed  CAS  Google Scholar 

  46. Rennick D, Yan G, Gemmell L, et al: Control of hemopoiesis by a bone marrow stromal cell line clone: lipopolysaccharide and interleukin-1 inducible production of colony-stimulating factors. Blood 1987;70:682–691.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Supported by research grants from the National Institutes of Health — CA39851. CA40818 and PO1HD19767.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Greenberger, J.S., FitzGerald, T.J. & Anklesaria, P. Recent studies of the hematopoietic microenvironment in long-term bone marrow cultures. Immunol Res 8, 226–235 (1989). https://doi.org/10.1007/BF02918147

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02918147

Keywords