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A newly established human acute lymphoblastic leukemia cell line with characteristics of the earliest B-cell maturation

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Virchows Archiv B

Summary

A new human acute lymphoblastic leukemia (ALL) cell line, designated HBL-3, was established from the bone marrow of a patient with non-T-ALL. The HBL-3 cell line expressed B4 (CD 19), BA-1 (CD 24) and HLA-DR antigens, but not surface immunoglobulin (SIg) or cytoplasmic immunoglobulin (CIg). The cell line lacked the common acute lymphoblastic leukemia antigen (CALLA) and antigenic markers characteristic of T-cell and myeloid cell lineages. The HBL-3 cells had structural rearrangements of both the homologous chromosome 9s, including a translocation with chromosome 1 which has been reported in a patient with common ALL. The cell line had rearranged immunoglobulin heavy chain genes but retained germ-line κ light chain genes and germ-line T-cell receptorβ- and γ-chain genes. The HBL-3 cell line was strongly positive for terminal deoxynucleotidyl transferase (TdT). These findings indicate that the HBL-3 cell line is derived from the earliest B-cell committed to B-cell lineage.

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Authors and Affiliations

  1. The Department of Pathology, Fukushima Medical College, Hikari-ga-Oka, 960-12, Fukushima, Japan

    Masafumi Abe, Naoya Nakamura & Haruki Wakasa

  2. First Division of Internal Medicine, Faculty of Medicine, Kyoto University, Japan

    Shirou Fukuhara & Takamasa Hayashi

  3. Second Division of Internal Medicine, Faculty of Medicine, Mie University, Japan

    Keiki Kawakami & Kenkichi Kita

  4. Department of Virology, Cancer Institute, Hokkaido University, Japan

    Toshifumi Kinoshita & Toyoro Ohsato

Authors
  1. Masafumi Abe
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  2. Naoya Nakamura
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  3. Shirou Fukuhara
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  4. Takamasa Hayashi
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  5. Keiki Kawakami
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  6. Kenkichi Kita
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  7. Toshifumi Kinoshita
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  8. Toyoro Ohsato
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  9. Haruki Wakasa
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Abe, M., Nakamura, N., Fukuhara, S. et al. A newly established human acute lymphoblastic leukemia cell line with characteristics of the earliest B-cell maturation. Virchows Archiv B Cell Pathol 59, 107–113 (1990). https://doi.org/10.1007/BF02899394

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  • DOI: https://doi.org/10.1007/BF02899394

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