Skip to main content
Log in

Demonstration of bacterial antigen in macrophages in experimental pyelonephritis

  • Published:
Virchows Archiv B

Summary

The immunomorphological characteristics of interstitial macrophages with PAS-positive granules were studied in experimentalEscherichia coli pyelonephritis in rats, using an anti-E. coli antibody. Immunohistochemical, immunoelectron microscopical, as well as light- and electron microscopical findings were compared at twelve time points between 2 days and 13 weeks after infection. Macrophages with PAS-positive granules were present in the inflammatory infiltrates from the 7th day. The granules were phagolysosomes, filled pre-dominantly with myelin figures. The myelin figures originated mainly from the constituents of the bacterial wall and reacted with the anti-E. coli antibody even 13 weeks after infection. The storage of bacterial residues with preserved antigenic structures for several weeks after infection indicates disturbed phagolysosomal elimination of the bacterial substances in the PAS-positive macrophages. In the formation of macrophages with PAS-positive granules, lysosomal overloading with large amounts of bacteria and cell debris is assumed, leading to consumption of the lysosomal enzymes, consequent incomplete breakdown and retention of the bacterial residues.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bourne JA (1983) Handbuch der Immunperoxidase-Färbemethoden. Dako Corporation, Carpinteria, USA

    Google Scholar 

  • Churg J, Cotran RS, Sinniah R, Sakaguchi H, Sobin LH (1985) Renal disease. Classification and atlas of tubulo-interstitial diseases. Igaku-Shoin Ltd, Tokyo, pp 34–35

    Google Scholar 

  • Bille J, Glauser MP (1982) Protection against chronic pyelonephritis in rats by suppression of acute suppuration: effect of colchicine and neutropenia. J Infect Dis 146:220–226

    PubMed  CAS  Google Scholar 

  • Cotran RS (1969) The renal lesion in chronic pyelonephritis: immunofluorescent and ultrastructural studies. J Infect Dis 120:109–117

    PubMed  CAS  Google Scholar 

  • Csapó Zs, Kuthy E, Lantos J, Ormos J (1975) Experimentally induced malakoplakia. Am J Pathol 70:453–464

    Google Scholar 

  • Cybulsky MI, Chan MKW, Movat HZ (1988) Acute inflammation and michrothrombosis induced by endotoxin, interleukin-1, and tumor necrosis factor and their implication in Gram-negative infection. Lab Invest 58:365–378

    PubMed  CAS  Google Scholar 

  • Ghadially FN (1988) Ultrastructural pathology of the cell and matrix. 3rd edition, vol II. Butterworths, London, pp 589–628

    Google Scholar 

  • Heptinstall RH (1983) Pathology of the kidney, vol III. Little, Brown and Co, Boston, pp 1381–1388

    Google Scholar 

  • Hill GS, Clark RL (1972) A comparative angiographic, microangiographic, and histologic study of experimental pyelonephritis. Invest Radiol 7:33–47

    PubMed  CAS  Google Scholar 

  • Iványi B, Ormos J, Lantos J (1983) Tubulointerstitial inflammation, cast formation, and renal parenchymal damage in experimental pyelonephritis. Am J Pathol 113:300–308

    PubMed  Google Scholar 

  • Iványi B, Krenács T, Petri S (1985) Phagocytosis of bacteria by proximal tubular epithelium in experimental pyelonephritis. Virchows Arch [B] 50:59–70

    Google Scholar 

  • Iványi B, Thoenes W (1987) Microvascular injury and repair in acute human bacterial pyelonephritis. Virchows Arch [A] 411:257–265

    Google Scholar 

  • Iványi B, Rumpelt HJ, Thoenes W (1988) Acute human pyelonephritis: Leukocytic infiltration of tubules and localization of bacteria. Virchows Arch [A] 414:29–37

    Google Scholar 

  • Krenács T, Iványi B, Csókási Zs, Petri S, Lantos J (1985) Escherichia coli ellenes immunsavó készítése és immunhisztoké-miai felhasználása kísérletes pyelonephritis morfológiai vizsgálatához. Kisérletes Orvostudomány 37:243–247

    Google Scholar 

  • Krenács T, Dobó E, Iványi B, Lászik Z (1988) Immunarany konjugátum készítése és felhasználási lehetóségei a fény-és elektronmikroszkópos immunhisztokémiában. Morphológiai és Ig Orv Szemle 28:29–38

    Google Scholar 

  • Katoh R, Ishizaki T, Tomichi N, Yagawa K, Kurihara H (1989) Malakoplakia of the thyroid gland. Am J Clin Pathol 92:813–820

    PubMed  CAS  Google Scholar 

  • Khalyl-Mawad J, Alba Greco M, Schinella RA (1982) Ultrastructural demonstration of intracellular bacteria in xanthogranulomatous pyelonephritis. Hum Pathol 13:41–47

    Article  PubMed  CAS  Google Scholar 

  • Langer KH, Thoenes W (1981) Characterization of cells involved in the formation of granuloma. Virchows Arch [B] 36:177–194

    Article  CAS  Google Scholar 

  • Maunsbach AB (1966) The influence of different fixatives and fixation methods on the structure of rat kidney proximal tubule cells. J Ultrastruct Res 15:242–282

    Article  PubMed  CAS  Google Scholar 

  • McClure J (1983) Malakoplakia. J Pathol 140:275–330

    Article  PubMed  CAS  Google Scholar 

  • Miller T, Phillips S (1981) Pyelonephritis: the relationship between infection, renal scarring, and antimicrobial therapy. Kidney Int 19:654–662

    Article  PubMed  CAS  Google Scholar 

  • Møller J Chr, Kristensen IB (1980) Xanthogranulomatous pyelonephritis. Acta Path Microbiol Scand Sect A 88:89–96

    Google Scholar 

  • Ormos J, Csapó Zs, Lantos J (1968) Contributions to the development of experimental pyelonephritis. Acta Morphol Acad Sci Hung 16:85–101

    PubMed  CAS  Google Scholar 

  • Ormos J, Mohácsi G, Kuthy E, Bóti Zs (1979) Renal cortical tubules in experimental malakoplakia. Phagocytic alteration of tubular epithelium. Exp Pathol 17:3–11

    CAS  Google Scholar 

  • Parsons MA, Harris SC (1983) Xanthogranulomatous pyelonephritis: a pathological, clinical and aetiological analysis of 87 cases. Diagn Histopathol 6:203–219

    PubMed  CAS  Google Scholar 

  • Povysil C (1976) Ultrastructure of experimental xanthogranulomatous pyelonephritis. Virchows Arch [B] 20:155–167

    CAS  Google Scholar 

  • Risdon RA (1989) Pyelonephritis and reflux nephropathy. In: Tisher CC, Brenner BM (eds) Renal pathology with clinical and functional correlations, vol I. JB Lippincott Co, Philadelphia, pp 799–802

    Google Scholar 

  • Rubin RH, Tolkoff-Rubin NE, Contran RS (1986) Urinary tract infection, pyelonephritis, and reflux nephropathy. In: Brenner BM, Rector FC (eds) The kidney. 3rd edition, vol II. WB Saunders Co, Philadelphia, pp 1127–1129

    Google Scholar 

  • Sanford JP, Hunter BW, Donaldson P (1962) Localization and fate of Escherichia coli in hematogenous pyelonephritis. J Exp Med 116:285–294

    Article  PubMed  CAS  Google Scholar 

  • Schubert GE (1984) Niere und ableitende Harnwege. In: Remmele W (ed) Pathologie 3. Springer, Berlin Heidelberg New York Tokyo Honkong, pp 53–54

    Google Scholar 

  • Tan HK, Heptinstall RH (1969) Experimental pyelonephritis. A light and electron microscopy study of the periodic acid-Schiff positive interstitial cell. Lab Invest 20:62–69

    PubMed  CAS  Google Scholar 

  • Wilson DR (1980) Pathophysiology of obstructive nephropathy. Kidney Int 18:281–292

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Iványi, B., Krenács, T., Dobó, E. et al. Demonstration of bacterial antigen in macrophages in experimental pyelonephritis. Virchows Archiv B Cell Pathol 59, 83–88 (1990). https://doi.org/10.1007/BF02899391

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02899391

Key words