Abstract
The fluorescence brightener Rylux BSU (RBSU) showed an affinity for polysaccharide components of cell walls and accumulated in the extension zones of hyphal apices inBasidiobolus ranarum. It inhibited the polarized growth of mycelial hyphae and induced isotropic growth resulting in spherical thick-walled cells up to 456 µm in diameter. On the inner cell wall surface, massive protuberances were formed. The cell wall and protuberances were positive in PAS and the Grocott method and stained with fluorochromes Blankophor BA, Calcofluor, Uvitex 2B, Rylux BSU and FITC-labeled WGA- and ConA-lectins. The WGA-FITC fluorescence intensity of the wall’s outermost layer, if not connected with neighbouring cells, and the fluorescence intensity of the innermost layer and of some protuberances mainly in their apical parts were on the average twice higher than the fluorescence intensity of the remaining wall material. RBSU binding to the cell wall material was stable. The process of converting from polarized to isotropic growth was reversible, depending upon contact with RBSU-containing medium. Repeated transfers of cells from RBSU-containing medium to an RBSU-free medium resulted in the development of apical swollen dumbbell-shaped cells.
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References
Colwin J.R., Witter D.E.: Congo red and calcofluor white inhibition ofAcetobacter xylinum cell growth and of bacterial cellulose microfibril formation: isolation and properties of a transient, extracellular glucan related to cellulose.Protoplasma 116, 34–40 (1983).
Grigoriu D., Delacrétaz J., Borelli D.:Medical Mycology. Hans Huber Publ., Toronto-Lewiston-New York-Bern-Stuttgart 1987.
Hejtmánek M., Koďousek R., Heitmánková N.: Fluorescence microscopical detection of pathogenic fungi with Rylux BSU.Čs.Patol. 25, 244–250 (1989)
Hejtmánek M., Doležel J., Holubová I.: Staining of fungal cell walls with fluorescent brighteners: flow-cytometric analysis.Folia Microbiol. 35, 437–442 (1990).
Otčenášek M., Hejtmánek M., Manych J., Tomšíková A.:Investigative Methods in Mycotic Diseases. Avicenum, Prague 1990.
Pearse A.G.E.:Histochemistry, Theoretical and Applied. J. & A. Churchill Ltd., London 1960.
Roncero C., Durán A.: Effect of calcofluor white and congo red on fungal cell wall morphogenesis:in vivo activation of chitin polymerization.J.Bacteriol. 163, 1180–1185 (1985).
Roncero C., Valdivieso M.H., Ribas J.R., Durán A.: Effect of calcofluor white on chitin synthases fromSaccharomyces cerevisiae.J.Bacteriol. 170, 1945–1949 (1988).
Szaniszlo P.J. (Ed.):Fungal Dimorphism: with Emphasis onFungi Pathogenic for Humans. Plenum Press, New York—London 1985.
Wessels J.G.H.: Cell wall synthesis in apical hyphal growth.Int.Rev.Cytol. 104, 37–79 (1986).
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Hejtmánek, M., Koďousek, R. & Raclavský, V. The fluorescence brightener Rylux BSU induces dimorphism inBasidiobolus ranarum . Folia Microbiol 38, 395–398 (1993). https://doi.org/10.1007/BF02898764
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DOI: https://doi.org/10.1007/BF02898764