biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 38:605-609, 1996 | DOI: 10.1007/BF02890616

Hardening by abscisic acid of tobacco plantlets grownin vitro

J. Pospíšilová1
1 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Czech Republic

Tobacco plantlets were grownin vitro on Murashige and Skoog's medium with2 % of saccharose. Addition of 0.01 mM abscisic acid (ABA) into the medium decreased stomatal conductance of the adaxial epidermis and especially the abaxial epidermis without negative effects on growth parameters. As a result the rate of water loss from ABA-treated plantlets taken out of cultivation vessels was slower than that of control plantlets. This could help their acclimation after transplantation toex vitro conditions.

Keywords: Nicotiana tabacum; stomatal conductance; transpiration curves

Received: May 7, 1996; Accepted: June 16, 1996; Published: December 1, 1996  Show citation

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Pospíšilová, J. (1996). Hardening by abscisic acid of tobacco plantlets grownin vitro. Biologia plantarum, 38(4), 605-609. doi: 10.1007/BF02890616
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    References

    1. Buddendorf-Joosten, J.M.C., Woltering, E.J.: Components of the gaseous environment and their effects on plant growth and development in vitro. - Plant Growth Regul.15: 1-16. 1994. Go to original source...
    2. Capellades, M., Fontarnau, R., Carulla, C., Debergh, P.: Environment influences anatomy of stomata and epidermal cells in tissue-culturedRosa multiflora. - J. amer. Soc. hort. Sci.115: 141-145, 1990. Go to original source...
    3. Cassells, A.C., Walsh, C: The influence of gas permeability of the culture lid on calcium uptake and stomatal function inDianthus microplants. - Plant Cell Tissue Organ Cult.37:171-178, 1994. Go to original source...
    4. Colonguasp, W., Nell, T.A., Kane, M.E., Barrett, J.E.: Effects of abscisic acid onex vitro acclimatization ofAronia arbutifolia (L.) Pers..- J. amer. Soc. hort. Sci.121: 101-104, 1996. Go to original source...
    5. Davies, W.J., Jones, H.G.: Abscisic Acid. Physiology and Biochemistry. - BIOS Scientific Publishers, Oxford 1991. Go to original source...
    6. Deng, R., Donnelly, D.J.:In vitro hardening of red raspberry through CO2 enrichment and relative humidity reduction on sugar-free medium. - Can. J. Plant Sci.73: 1105-113, 1993. Go to original source...
    7. Eberhardt, H.-J., Wegmann, K.: Effect of abscisic acid and proline on adaptation of tobacco callus cultures to salinity and osmotic shock. - Physiol. Plant.76: 283-288, 1989. Go to original source...
    8. Eliasson, M.K., Beyl, C.A., Barker, P.A.:In vitro responses and acclimatizationof Prunus serotina with paclobutrazol. - J. Plant Growth Regul.13: 137-142, 1994. Go to original source...
    9. Emons, A.M.C., Samallo-Droppers, A., Van der Toorn C: The influence of sucrose, mannitol, L-proline, abscisic acid and gibberellic acid on the maturation of somatic embryos ofZea mays L. from suspension cultures. - J. Plant Physiol.142: 597-604, 1993. Go to original source...
    10. Etienne, H., Montoro, P., Michaux-Ferriere, N., Carron, M.P.: Effect of desiccation, medium osmolality and abscisic acid on the maturation ofHevea brasiliensis somatic embryos. - J. exp. Bot.44: 1613-1619, 1993. Go to original source...
    11. Ghashghaie, J., Brenckmann, F., Saugier, B.: Water relations and growth of rose plants culturedin vitro under various relative humidities. - Plant Cell Tissue Organ Cult.30: 51-57, 1992. Go to original source...
    12. Kozai, T., Smith, M.A.L.: Environmental control in plant tissue culture - general introduction and overview. - In: Aitken-Christie, J., Kozai, T., Smith, M.L. (ed.): Automation and Environmental Control in Plant Tissue Culture. Pp. 301-318. Kluwer Academic Publishers, Dordrecht - Boston - London 1995. Go to original source...
    13. Misra, S., Attree, S.M., Leal, I., Fowke, L.C.: Effect of abscisic acid, osmoticum, and desiccation on synthesis of storage proteins during the development of white spruce somatic embryos. - Ann. Bot.71: 11-22,1993. Go to original source...
    14. Pospišilová, J.: Effect of air humidity on the development of functional stomatal apparatus. - Biol. Plant.38: 197-204, 1996. Go to original source...
    15. Pospišilová, J., Čatský, J., Šestäk, Z.: Photosynthesis in plants cultivatedin vitro. -In: Pessarakli, M. (ed.): Handbook of Photosynthesis. Pp. 525-540. Marcel Dekker, New York 1996.
    16. Pospišilová, J., Čatský, J., Synková, H., Macháčková, I., Solárovä, J.: Gas exchange andin vivo chlorophyll fluorescence in potato and tobacco plantletsin vitro as affected by various concentrations of 6-benzylaminopurine. - Photosynthetica29: 1-12, 1993.
    17. Pospišilová, J., Solárovä, J., Čatský, J.: Photosynthetic responses to stresses duringin vitro cultivation. - Photosynthetica26: 3-18, 1992.
    18. Roberts, A.V., Smith, E.F., Mottley, J.: The preparation of micropropagated plantlets for transfer to soil without acclimatization.- In: Pollard, J.W., Walker, J.M. (ed.): Methods in Molecular Biology. Vol. 6. Plant Cell and Tissue Culture. Pp. 227-236. Humana Press, Clifton 1990. Go to original source...
    19. Sallanon, H., Maziere, Y.: Influence of growth room and vessel humidity on thein vitro development of rose plants. -Plant Cell Tissue Organ. Cult.30: 121-125, 1992. Go to original source...
    20. Short, K.C., Warburton, J., Roberts, A.V.:In vitro hardening of cultured cauliflower andChrysanthemum plantlets to humidity. - Acta Hort.212: 329-334, 1987. Go to original source...
    21. Smith, E.F., Roberts, A.V., Mottley, J.: The preparationin vitro chrysanthemum for transplantation to soil. 3. Improved resistance to desiccation conferred by reduced humidity. - Plant Cell Tissue Organ Cult.21: 141-145, 1990. Go to original source...
    22. Smith, E.F., Gribaudo, I., Roberts, A.V., Mottley, J.: Paclobutrazol and reduced humidity improve resistance to wilting of micropropagated grapevine. - HortScience27: 111-113, 1992. Go to original source...
    23. Solárová, J., Součková, D., Ullmann, J., Pospišilová, J.:In vitro culture: environmental conditions and plantlet growth as affected by vessel type and stopper material. - Zahradnictvi23(2): 51-58, 1996.
    24. Wardle, K., Dobbs, E.B., Short, K.C.:In vitro acclimatization of aseptically cultured plantlets to humidity. - J. amer. Soc. hort. Sci. 108: 386-389, 1983. Go to original source...
    25. Yue, D., Gosselin, A., Desjardins, Y.: Effect of forced ventilation at different relative humidities on growth, photosynthesis and transpiration of geranium plantletsin vitro. - Can. J. Plant Sci.73: 249-256, 1993. Go to original source...
    26. Zaid, A., Hughes, H.: Water loss and polyethylene glycol-mediated acclimatization ofin vitro-grown seedlings of 5 cultivars of date palm(Phoenix dactylifera L.) plantlets. - Plant Cell Rep.14: 385- 388, 1995. Go to original source...
    27. Ziv, M.:In vitro hardening and acclimatization of tissue culture plants. - In: Withers, L.A., Alderson, P.G. (ed.): Plant Tissue Culture and its Agricultural Applications. Pp. 187-196. Butterworths, London - Boston - Durban -Singapore - Sydney - Toronto - Wellington 1986. Go to original source...

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