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Introduction of a rice blight resistance gene,Xa21, into five Chinese rice varieties through anAgrobacterium-mediated system

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Abstract

A cloned gene,Xa21 was transferred into five widely-used Chinese rice varieties through anAgrobacterium- mediated system, and over 110 independent transgenic lines were obtained. PCR and Southern analysis of transgenic plants revealed the integration of the wholeXa21 gene into the host genomes. The integratedXa21 gene was stably inherited, and segregated in a 3: 1 ratio in the selfed T1 generation when one copy of the gene was integrated in the transformants. Inoculation tests displayed that transgenic T0 plants andXa21 PCR-positive T1 plants were highly resistant to bacterial blight disease. The selectedXa21 homozygous resistant transgenic lines with desirable qualities may be propagated as new varieties or utilized in hybrid rice breeding.

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Authors and Affiliations

  1. Institute of Genetics, Chinese Academy of Sciences, 100101, Beijing, China

    Wenxue Zhai, Xiaobing Li, Wenzhong Tian, Shouyun Cao, Xianfeng Zhao, Bin Zhao & Lihuang Zhu

  2. Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences, 100080, Beijing, China

    Yongli Zhou & Qi Zhang

  3. Agricultural College, Yangzhou University, 225009, Yangzhou, China

    Xuebiao Pan

Authors
  1. Wenxue Zhai
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  2. Xiaobing Li
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  3. Wenzhong Tian
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  4. Yongli Zhou
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  5. Xuebiao Pan
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  6. Shouyun Cao
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  7. Xianfeng Zhao
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  8. Bin Zhao
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  9. Qi Zhang
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  10. Lihuang Zhu
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Correspondence to Lihuang Zhu.

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Zhai, W., Li, X., Tian, W. et al. Introduction of a rice blight resistance gene,Xa21, into five Chinese rice varieties through anAgrobacterium-mediated system. Sci. China Ser. C.-Life Sci. 43, 361–368 (2000). https://doi.org/10.1007/BF02879300

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  • DOI: https://doi.org/10.1007/BF02879300

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