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Use of the isopiestic method to determine the osmotic pressure of concentrated nutrient solutions for the cultivation of osmophilic microorganisms

Применение изопиестического метода для определения осмотического давления концентриро ванных питательных растворов для культивирования осмофиль ных микроорганизмов

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Abstract

The isopiestic method was used to determine the osmotic pressure of sucrose, glucose, ethylene glycol, potassium chloride and sodium sulphate solutions used to raise the osmotic pressure of cultivation media. The method is suitable for determining π of concentrated solutions. In solutions with NaCl concentrations of less than 0.1m, the accuracy of the method fell because of the relatively high water surface tension and the low vapour pressure. The osmophilic character ofCandida utilis yeasts adapted to dense recirculated molasses mashes was studied by cultivation on synthetic medium whose osmotic pressure was raised by adding mineral salts and organic substances.

Abstract

Изопиестический метод был использован для определения осмотического давления растворов сахарозы, глюкозы, этиленгликоля, хлористого калия и сернокислого натрия, применяемых в качестве присадки для повышения осмотического давления культивационной среды. Метод удобен для определения осмотического давления концентрированных растоворов. При концентрации раствора ниже 0,1m NaCl точность метода уменьшалась в связи со сравнительно высоким поверхностным натяжением воды и низким давлением водяных паров.—Путем выращивания в синтетической среде с повышенным осмотическим давлением, обусловленным присадками минеральных солей и органических веществ, изучали осмофильный характер дрожжей Candida utilis, адаптированных к густым рециркулированным паточным заторам.

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  1. Research Institute for Fermentation and Canning Industry, Prague

    Alena Čejková

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  1. Alena Čejková
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Čejková, A. Use of the isopiestic method to determine the osmotic pressure of concentrated nutrient solutions for the cultivation of osmophilic microorganisms. Folia Microbiol 10, 246–250 (1965). https://doi.org/10.1007/BF02875954

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  • DOI: https://doi.org/10.1007/BF02875954

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