Abstract
We studied growth and cell division ofEscherichia coli 15 TAU after transfer to thymine-free medium with different sources of carbon and energy or to the same medium in which not only thymine but also arginine and/or uracil were omitted. After transfer to thymine-free medium only a fraction of cells divides once. The size of the dividing fraction is predetermined particularly by conditions of balanced growth before inhibition of DNA synthesis and only slightly affected by conditions after transfer, while the growth rate after shift to medium with different source of carbon and energy changes abruptly. Following transfer to arginine-deficient media cell division proceeds much more slowly than in other cases tested. The fraction of cells which causes a deviation of rate maintenance after shift-up and shift-down (Cooper, 1969) seems to be the same as the cell fraction dividing after transfer to thymine-free medium.
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Opekarová, M., Vondrejs, V. Growth and cell division ofEscherichia coli 15 TAU after transfer to deficient media with different sources of carbon and energy. Folia Microbiol 19, 264–271 (1974). https://doi.org/10.1007/BF02873218
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DOI: https://doi.org/10.1007/BF02873218