Abstract
Continuous flow technique was used to study the formation of organic acids and of carbon dioxide during anaerobic breakdown of glucose in soil. Carbon dioxide, formic, acetic, butyric and lactic acids were the main products of anaerobic decomposition of glucose. However, succinic acid, α-ketoglutaric acid and fumarie or glutarie acids could be detected also under certain circumstances. Two types of glucose fermentation apparently occurred during continuous addition of glucose to the soil. The mixed acid fermentation of glucose prevailed at the later stage. Simultaneous addition of mineral nitrogen and phosphorus with glucose stimulated the conversion of organic acids to methane in soil exhibiting high capacity of methane-forming bacteria.
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References
Alexander, M.:Introduction to Soil Microbiology. J. Wiley & Sons., Inc. New York and London, 1961.
Bryant, F., Overell, B. T.:Quantitative chromatography analysis of organic acids in plant tissue extract. Biochim. biophys. Acta 10: 471, 1953.
Bullen, W. A., Varner, J. E., Burell, R. C.:Separation of organic acids from plant tissue. Chromatographic technique. Anal. Chem. 24: 187, 1952.
Greenwood, D. J.:The effect of oxygen concentration on the decomposition of organic materials in soil. Plant and Soil14: 360, 1961.
Greenwood, D. J.: Goodman, D.:Direct measurements of the distribution of oxygen in soil aggregates and in columns of fine soil crumbs. J. Soil Sci. 18: 182, 1967.
Hais, J. M., Macek, K.:Paper Chromatography. Publ. House Czechoslov. Acad. Sci. Prague, 1963.
Macura, J.:Continuous flow method in soil microbiology. I. Apparatus. Fol. microbiol. 6: 328, 1961.
Macura, J., Málek, I.:Continuous-flow Method for the Study of Microbiological Processes in Soil Samples. Nature, 182: 1796, 1958.
Schwartz, S. M., Varner, J. E., Martin, W. P.:Separation of organic acids from several dormant and incubated soils. Soil Sci. Soc. Am. Proc. 18: 174, 1954.
Stephenson, M.:Bacterial metabolism. London, Longmans, Green & Co. London, 1949.
Takai, A., Kamura, T.:The mechanism of reduction in waterlogged paddy soil. Fol. microbiol.11: 304, 1966.
Vančura, V., Macura, J., Szolnoki, J.:The products of glucose metabolism in the soil. Transaction 8th Intern. Congress of Soil Science. Vol. III p. 779, Bucharest, Publ. House Acad. Soc. Rep. Romania 1964.
Watson, D.:Enzymic determination of glucose and easily hydrolyzable glucose esters in blood. Anal. Biochem. 3: 131, 1962.
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Dedicated to Academician Ivan Málek on the occasion of his 60th birthday
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Takai, Y., Macura, J. & Kung, F. Anaerobic decomposition of glucose continuously added to the soil. Folia Microbiol 14, 327–333 (1969). https://doi.org/10.1007/BF02872700
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DOI: https://doi.org/10.1007/BF02872700