Abstract
The formation of colour mutations ofSerratia marcescens by the action of chloramphenicol was studied. Pink variants were the commonest; the proportion of white variants was much smaller. Almost 100% mutations were formed in a two-day culture containing 100 μg. chloramphenicol/ml. Comparative experiments showed that the change in pigment formation was hereditary, i.e. that actual mutation, and not selection of chloramphenicol-resistant mutants, occurred. Mutation occurred both in strain 151 and strain HY. The resultant mutations remained constant throughout ten passages on normal nutrient medium. The minimum chloramphenicol concentration which produced an increase in the mutation frequency was 5 μg./ml. The combined effect of X-ray irradiation and chloramphenicol treatment somewhat stimulated the increase in the frequency of mutation as compared with cells which were only irradiated. The increase in the frequency of mutation was much slower on solid medium containing chloramphenicol.
Abstract
Изучалось возникновение цветных мутантов Serratia marcescens под действием хлорамфеникола. Наиболее часто встречались розовые, в гораздо меньшей степени—белые варианты. В 2-дневной культуре, содержавшей 100 μг/млхлорамфеникола, возникло почти 100% мутаций. Сравнительные обыть показали, что речь идет о наследственном изменении в образовании пигемента, т. е. о мутации, а не о простой селекции хлорамфениколорезистентных мутантов. Мутации встречались у штамма № 151 из коллекции г. Брно и у штамма HY американского музея. Полученные мутанты окаазались устойчивыми в ходе 10 пересевов на нормальной питательной среде. Минимальная концентрация хпорамфеникола, которая способна вызвать повышение частоты случаев мутаций составляла 5 μг/мЛ. Сочетание действия рентгеновского облучения и хлорамфеникола несколько ускоряло учащение мутаций по сравнению с клетками, которые только облучалися. В культурах на плотных средах с хлорамфениколом количество мутаций возрастает гораздо медленнее.
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Janovská, E., Herčík, F., Vlašínová, M. et al. Induction of mutations inSerratia marcescens by a proteosynthesis block. Folia Microbiol 8, 293–300 (1963). https://doi.org/10.1007/BF02868773
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DOI: https://doi.org/10.1007/BF02868773