Abstract
The yeast phosphomannan (PM) derived fromHansenula capsulata strain exerts an inhibitory effect on thein vitro bactericidal activity of fresh sera of newborn, colostrum-deprived germ-free piglets to rough strains ofEscherichia coli (S-16 and Lilly). The experiments presented indicate that the PM function probably takes place at the C1 level. The inhibitory effect of PM does not occur provided bacteria are sensitized by specific antiserum prior to exposure to piglet serum. The antibody which was responsible for removal of PM blockade was of 19S nature, 2-mercaptoethanol-sensitive and can be absorbed by heat inactivated bacteria (roughEscherichia coli) or inhibited by addition of soluble somatic antigen (endotoxin) obtained from the same strain ofEscherichia coli (rough). The possible mechanism of inhibition of bactericidal activity by PM is discussed.
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This investigation was done in the Laboratory of Dr. M. A. Leon, Pathology Research, St. Luke's Hospital, Cleveland, Ohio, U.S.A.
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Miler, I., Milerová, J. & Leon, M.A. The inhibition of bactericidal activity of sera of newborn germ-free piglets to roughEscherichia coli by yeast mannan. Folia Microbiol 15, 1–8 (1970). https://doi.org/10.1007/BF02867041
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DOI: https://doi.org/10.1007/BF02867041