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Plant regeneration from leaf tissues of four North Dakota genotypes of potato (Solanum tuberosum L.)

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Abstract

An efficient plant regeneration system was developed fromin vitro leaf tissues of four North Dakota potato genotypes. The best medium for genotype ND860-2 was Murashige and Skoog medium with 20.0 μM IAA and 11.4 μM zeatin riboside. Two to three weeks of initial dark treatments had a significant effect in reducing the time for shoot regeneration and increasing the number of regenerated shoots. Four antibiotics commonly used inAgrobacterium- mediated transformation were tested for their effects on shoot regeneration. Shoot induction was completely inhibited by kanamycin at 15 mg/L and hygromycin at 4 mg/L or higher, but not by carbenicillin (except at 1000 mg/L) and cefotaxime. Hygromycin significantly stimulated shoot induction at 1 mg/L.

Compendio

Se desarrolló un sistema eficiente de regeneratión vegetal utilizando tejidos in vitro de hojas de cuatro genotipo de papa de North Dakota. El mejor medio para el genotipo ND860-2 fue el de Murashige y Skoog con 20.0 μM IAA y 11.4 μM de zeatin riboside. Tratamientos iniciales de dos a tres semanas en la oscuridad tuvieron un efecto signifïcativo en reducir el tiempo para la regeneratión del brote y en el incremento del número de brotes regeneradores. Se probaron cuatro antibióticos comunmente utilizados en la transformatión por medio del Agrobacterium, para sus efectos sobre la regeneratión de brotes. La induction de brotes fue completamente inhibida por kanamycin a 15 mg/L e hygromycin a 4 o más mg/L, pero no por carbenicillin (con la exceptión a 1,000 mg/L) y cefotaxime. A1 mg/L, hygromycin estimuló signifïcativamente la inductión de brotes.

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Authors and Affiliations

  1. Austrian Academy of Sciences, Institute of Molecular Biology, Billrothtrasse 11, A-5020, Salzburg, Austria

    Y. D. Park

  2. McCain Produce Inc., EOJ 1K0, Florenceville, N.B., Canada

    D. H. Ronis

  3. 1125 N.E. First St., 55021, Faribault, MN

    A. A. Boe

  4. Department of Plant Sciences, North Dakota State University, 58105, Fargo, ND

    Z. M. Cheng

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  1. Y. D. Park
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  2. D. H. Ronis
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  3. A. A. Boe
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  4. Z. M. Cheng
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Park, Y.D., Ronis, D.H., Boe, A.A. et al. Plant regeneration from leaf tissues of four North Dakota genotypes of potato (Solanum tuberosum L.). American Potato Journal 72, 329–338 (1995). https://doi.org/10.1007/BF02849330

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  • DOI: https://doi.org/10.1007/BF02849330

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