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Multiple endoxylanases ofButyrivibrio sp.

  • 2nd ISAM (International Symposium on Anaerobic Microbiology) Anaerobic Microbiology: Centeral European Perspective held in Prague (Czechia) November 10–11, 2000
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Abstract

Butyrivibrio sp. Mz 5 with a high xylanolytic activity was isolated. Four major xylanases were detected in the cell-associated fraction using the zymogram technique. The xylanolytic activity was inducible with the oat spelts xylan; two endoxylanases (51 and 145 kDa) were formed constitutively. The bulk of the xylanolytic activity was cell-bound and growth-phase dependent; the maximum activity in the cell-associated fraction was achieved after 16 h of incubation. The highest xylanolytic activity was determined in a medium with 0.5% oat spelts xylan. Under optimum conditions (the highest xylanolytic activity produced), the two cell-bound xylanases (51 and 58 kDa) were isolated by anion exchange chromatography on CIM DEAE 8 tubes attached to a MPLC system, and gel filtration.

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References

  • Ha Y.W., Stack R.J., Hespell R.B., Gordon S.H., Bothast R.J.: Some chemical and physical properties of extracellular polysaccharides produced byButyrivibrio fibrisolvens strains.Appl. Environ. Microbiol.57, 2016–2020 (1991).

    PubMed  CAS  Google Scholar 

  • Hespell R.B., Wolf R., Bothast R.J.: Fermentation of xylans byButyrivibrio fibrisolvens and other ruminal bacteria.Appl. Environ. Microbiol.53, 2849–2853 (1987).

    PubMed  CAS  Google Scholar 

  • Lin L.L., Thompson J.A.: An analysis of extracellular xylanases and cellulases ofButyrivibrio fibrisolvens H17c.FEMS Microbiol. Lett.68, 197–203 (1991).

    Article  PubMed  CAS  Google Scholar 

  • Laemmli U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature227, 680–685 (1970).

    Article  PubMed  CAS  Google Scholar 

  • Lever M.: Carbonydrate determination with 4-hydroxybenzoic acid hydrazide (PAHBAH): effect of bismuth on the reaction.Anal. Biochem.81, 21–27 (1977).

    Article  PubMed  CAS  Google Scholar 

  • Mannarelli B.M.: Deoxyribonucleic acid relatedness among strains of the speciesButyrivibrio fibrisolvens.Internat. J. Syst. Bacteriol.38, 340–347 (1988).

    Article  Google Scholar 

  • Marinšek Logar R., Zorec M., Nekrep F.V.: Isolation and characterization ofButyrivibrio-like rumen bacteria possessing high xylanolytic activity.Reprod. Nutr. Dev.40, 191–192 (2000).

    Google Scholar 

  • Štrancar A., Barut M., Podgornik A., Koselj P., Schwinn H., Raspor P., Josić D.: Application of compact porous tubes for preparative isolation of clotting factor VIII from human plasma.J. Chromatogr. A760, 117–123 (1997).

    Article  PubMed  Google Scholar 

  • Williams A.G., Withers S.E.: Induction of xylan-degrading enzymes inButyrivibrio fibrisolvens.Curr. Microbiol.25, 297–303 (1992a).

    Article  CAS  Google Scholar 

  • Williams A.G., Withers S.E.: The regulation of xylanolytic enzyme formation byButyrivibrio fibrisolvens NCFB 2249.Lett. Appl. Microbiol.14, 194–198 (1992b).

    Google Scholar 

  • Wong C., Sridhara S., Bardwell J.C.A., Jakob U.: Heating greatly speeds Coomassie Blue staining and destaining.Biotechniques28, 426–432 (2000).

    PubMed  CAS  Google Scholar 

  • Wong K.K.Y., Tan L.U.L., Saddler J.N.: Multiplicity of β-1,4-xylanase in microorganisms: functions and applications.Microbiol. Rev.52, 305–317 (1988).

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Zootechnical Department, Biotechnical Faculty, University of Ljubljana, 1230, Domžale, Slovenia

    M. Zorec, T. Čepeljnik, F. V. Nekrep & R. Marinšek Logar

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  1. M. Zorec
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  2. T. Čepeljnik
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  3. F. V. Nekrep
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  4. R. Marinšek Logar
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Zorec, M., Čepeljnik, T., Nekrep, F.V. et al. Multiple endoxylanases ofButyrivibrio sp.. Folia Microbiol 46, 94–96 (2001). https://doi.org/10.1007/BF02825897

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  • DOI: https://doi.org/10.1007/BF02825897

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