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Plasmid shuttle vector with two insertionally inactivable markers for coryneform bacteria

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Abstract

A new shuttle vector pCEM500 replicating inEscherichia coli and inBrevibacterium flavum was constructed. It carries two antibiotic resistance determinants (Kmr/Gmr from plasmid pSa of Gram-negative bacteria and Smr/Spr from plasmid pCG4 ofCorynebacterium glutamicum) which are efficiently expressed in both hosts and can be inactivated by insertion of DNA fragments into the unique restriction endonuclease sites located within them. This vector was found to be stably maintained inB. flavum and can be used for transfer of the cloned genes into this amino-acid-producing coryneform bacterium.

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Authors and Affiliations

  1. Department of Microbial Genetics, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20, Prague 4

    J. Nešvera, M. Pátek, J. Hochmannová & P. Pinkas

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  1. J. Nešvera
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  2. M. Pátek
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  3. J. Hochmannová
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  4. P. Pinkas
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Nešvera, J., Pátek, M., Hochmannová, J. et al. Plasmid shuttle vector with two insertionally inactivable markers for coryneform bacteria. Folia Microbiol 35, 273–277 (1990). https://doi.org/10.1007/BF02821278

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  • DOI: https://doi.org/10.1007/BF02821278

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