Abstract
Disruption of genes encoding endogenous transport proteins inSaccharomyces cerevisiae has facilitated the recent cloning, by functional expression, of cDNAs encoding K+ channels and amino acid transporters from the plantArabidopsis thaliana [1–4]. In the present study, we demonstrate in whole-cell patch clamp experiments that the inability oftrk1Δtrk2Δ mutants ofS. cerevisiae to grow on submillimolar K+ correlates with the lack of K+ inward currents, which are present in wild-type cells, and that transformation of thetrk1Δtrk2Δ double-deletion mutant withKAT1 fromArabidopsis thaliana restores this phenotype by encoding a plasma membrane protein that allows large K+ inward currents. Similar K+ inward currents are induced by transformation of atrk1 mutant withAKT1 fromA. thaliana.
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Anderson J.A., Huprika S.S., Kochian L.V., Lucas W.J., Gaber R.F.:Proc. Nat. Acad. Sci. USA 89, 3736–3740 (1992).
Sentenac H., Bonneaud N., Minet M., Lacroute F., Salmon J.-M., Gaymard F., Grignon C.:Science 256, 663–665 (1992).
Hsu L.-C., Chiou T.-J., Chen L., Bush D.R.:Proc. Nat. Acad. Sci. USA 90, 7441–7445 (1993).
Frommer W.B., Hummel S., Riesmeier J.W.:Proc. Nat. Acad. Sci. USA 90, 5944–5948 (1993).
Bertl A., Slayman C.L.:J. Exp. Biol. 132, 271–287 (1993).
Hamill O.P., Marty A., Neher E., Sakmann B., Sigworth F.J.:Pflügers Arch. 391, 85–100 (1981).
Bertl A., Gradmann D., Slayman C.L.:Phil. Trans. R. Soc. Lond. B338, 63–72 (1992).
Bertl A., Slayman C.L., Gradmann D.:J. Membr. Biol. 132, 183–199 (1993).
Blatt M.R.:J. Gen. Physiol. 99, 615–644 (1992).
Ramos J., Contreras P., Rodriguez-Navarro A.:Arch. Microbiol. 143, 88 (1985).
Schachtmann D.P., Schroeder J.I., Lucas W.J., Anderson J.A., Gaber R.F.:Science 258, 1654–1658 (1992).
Very A.-A., Bosseux C., Gaymard F., Sentenac H., Thibaud J.-B.:Pflügers Archiv. European J. Physiol. 428, 422–424 (1994).
MacKinnon R., Heginbotham L., Abramson T.:Neuron 5, 767–771 (1990).
Bertl A., Anderson J.A., Slayman C.L., Gaber R.F.:Proc. Nat. Acad. Sci. USA (submitted).
Ko, C.H., Gaber R.F.:Mol. Cell. Biol. 11, 4266–4273 (1991).
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This work was supported by a grant from theForschungsgemeinschaft (A.B.), TheU.S. Department of Energy (c.L.S.), The U.S. National Science Foundation (R.F.G.) Lisboa, Portugal.
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Bertl, A., Anderson, J.A., Slayman, C.L. et al. Inward and outward rectifying potassium currents inSaccharomyces cerevisiae mediated by endogenous and heterelogously expressed ion channels. Folia Microbiol 39, 507–509 (1994). https://doi.org/10.1007/BF02814074
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DOI: https://doi.org/10.1007/BF02814074