Abstract
The following complex nitrogen sources were evaluated for the production of penicillin acylase byBacillus megaterium: casein hydrolysate, corn steep liquor, stick water concentrate, blood meal and defatted sunflower meal. Experiments were run in shake flasks at 30‡C and pH 7.0. Best results were obtained with casein hydrolysate: 244 IU/I were produced with a productivity of 20.3 IU/l/h and yield of 717.6 IU/g of nitrogen. The lowest results correspond to sunflower meal with 39 IU/1.
This is a preview of subscription content, log in via an institution to check access.
References
Cole, M. 1969 Hydrolysis of penicillins and related compounds by the cell-bound penicillin acylase ofEscherichia coli.Biochemical Journal 155, 733–739.
Illanes, A., Acevedo, F., Gentina, J.C., Reyes, I., Torres, R., Cartagena, O., Ruiz, A. & Vásquez, M. 1994 Production of penicillin acylase fromBacillus megaterium in complex and defined media.Process Biochemistry 29, 263–270.
Murao, S. & Sakaguchi, K. 1964 Process for preparing 6-aminopenicillanic acid byBacillus megaterium.US Patent 3, 144–395.
Niedermayer, A. 1964 Determination of phenylacetic acid in penicillin fermentation media by means of gas chromatography.Analytical Chemistry 36, 938–939.
Shewale, J.G., Deshpande, B.S., Suhakaran, V.K. & Ambedkar, S.S. 1990 Penicillin acylases: Applications and potentials.Process Biochemistry International 25 (6), 97–103.
Shewale, J.G., Kumar, K. & Ambekar, G. 1987 Evaluation of determination of 6-aminopenicillanic acid by p-dimethylamino benzaldehyde.Biotechnology Techniques 1, 69–72.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gentina, J.C., Acevedo, F. & Villagra, M.P. Effect of complex nitrogen sources on the production of penicillin acylase byBacillus megaterium . World J Microbiol Biotechnol 13, 127–128 (1997). https://doi.org/10.1007/BF02770818
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02770818