Abstract
Environmental factors such as diet, physical activity, drugs, pollution and life style play an important role in the progression and/or precipitation of diseases like diabetes, hypertension, obesity and cardiovascular disorders. Indiscriminate use of antibiotics to combat infectious diseases is one of the commonest forms of misuse of drugs. Antibiotics seem to have a correlation with diabetes and pancreatic function. There are controversial reports about the effect of antibiotics on the pancreatic islets; some suggesting their harmless action, some depicting a beneficial role and others indicating deleterious effect. Moreover, use of antibiotics is mandatory during islet isolation and cultivation to reduce incidences of microbial contamination. It is likely that antibiotic treatment may adversely affect islet viability and its functioning leading to failure of islet transplantation. The presentin vitro study was undertaken to examine the effect of commonly used antibiotics such as gentamycin, penicillin, streptomycin, tetracycline, neomycin, erythromycin and chloramphenicol on islet viability, its functioning and induction of oxidative stress if any. The viability and insulin production data showed that none of the antibiotics used in the present study affect the viability and the functioning of the islets at their pharmacological concentrations. Free radical levels measured in terms of melonyldialdehyde (MDA), nitric oxide (NO) and reduced glutathione (GSH) reveal that except for a marginal increase in lipid peroxidation with tetracycline and slight increase in NO levels with streptomycin, none of these antibiotics affect the oxidative status of the cells. Antioxidant enzymes such as superoxide dismutase and catalase remain unaffected after this treatment. Our results reveal the innocuous nature of the antibiotics used at pharmacological concentrations, suggesting their safety whenever prescribed to combat infections and also during islet isolation procedures.
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Abbreviations
- DTNB:
-
5,5-dithiobis-2-nitrobenzoic acid
- DTZ:
-
dithiozone
- FCS:
-
foetal calf serum
- GSH:
-
glutathione
- KRBH:
-
Krebs-Ringer bicarbonate buffer (pH 7.4) with HEPES
- MDA:
-
melonyldialdehyde
- NO:
-
nitric oxide
- SOD:
-
superoxide dismutase
- STZ:
-
streptozotocin
References
Aebi H E 1984 Catalase; inMethods of enzymatic analysis (ed.) H V Bergmeyer (New York: Wiley) Vol. 3, pp 273–286
Albamunits V, Quintana F, Reshef T, Elias D and Cohen I R 1999 Acceleration of autoimmune diabetes by cyclophosphamide is associated with enhanced IFN-g secretion pathway;J Autoimmun. 13 383–392
Ames B N, Shigenaga M K and Hagen T M 1993 Oxidants, antioxidants and the degenerative process of aging;Proc. Natl. Acad. Sci. USA 90 7915–7922
Assan R, Assan D, Delaby J, Debussche X and Toublanc M 1993 Pentamide induced dysglycemia: experimental models in the rat;Diabetes Metabol. 19 262–272
Bastar I, Seckin S, Uysal M and Aykac-Toker G 1998 Effect of streptozotocin on glutathione and lipid peroxide levels in the various tissues of rat;Res. Commun. Mol. Pathol. Pharmacol. 102 265–272
Beauchamp C and Friddovich I 1971 Improved assays and an assay applicable to acrylamide Gels;Anal. Biochem. 44 276–287
Bhonde R R, Parab P B and Sheorin V S 1999 An “in vitro” model for rapid screening of insulin secretagogue activity of oral hypoglycemics;In vitro Cell. Dev. Biol. 35 366–368
Bresslers P and Defronzo R A 1994 Drugs and Diabetes;Diabetes Rev. 2 53–84
Boschero A C and Delattre E 1985 The mechanism of gentamycin inhibited insulin release by isolated islets;Arch. Int. Pharmacodyn. Ther. 273 167–176
Beuer G E and Noe B D 1985 Regulation of hormone biosynthesis in cultured islet cells from anglerfish;In vitro. Cel. Dev. Biol. 21 15–21
Dalpe-Scott M, Heick H M and Begin-Heick N 1983 Insulin secretion in obese (ob/ob) mouse. The effect of oxytetracyclin on insulin release;Diabetes 32 932–937
Dibas A I, Yorio T and Easom R A 1995 Gramicidin D is a potent insulin secretagogue: dependence on extracellular calcium influx;Biochem. Biophys. Res. Commun. 212 449–454
Hiramatsu S, Hoog A, Moller C and Grill V 2000 Treatment with diazoxide causes prolonged improvement of the β-cell function in rat islets transplanted to a diabetic environment;Metabolism 49 657–661
Jha T K and Sharma V K 1984 Pentamide induced diabetes mellitus;Trans. R. Soc. Trop. Med. Hyg. 78 252–253
Konings W T and Drijver E B 1979 Radiation effects on membranes I. Vitamin E Deficiency and Lipid peroxidation;Radiat. Res. 80 494
Lorenzo C, Delomo Martiner M I, Pastor L, Almara Z A, Belmonte A and Caropaton A 1999 Effect of oxytetracyclin on the rat endocrine pancreas;Int. J. Pancreatol. 26 181–188
Malaissie W J, Lebrun P, Pirotte B, Van Polje P D, Vinambers C, Vollanuevad, Pennacarrilo M L, Valverde I, Gabel J and Rorsman P 1996 The riddle of formycin — A insulinotropic action;Biochem. Mol. Med. 57 47–63
Poltorak V V 1984 Biosynthesis and secretion of insulin under the effect of an antibiotic of the tetracycline group;Probl. Endokrinol. (Mosk.) 30 57–61
Sai P, Boilloit D, Boitard C, Debray-Sacks M and Reach A R 1983 Pentamide, a new diabetogenic drug in laboratory rodents;Diabetologia 25 418–423
Samual A C, Kermit M B, Sandra S D, Thelma C R and William L L 1994 Staining andin vitro toxicity of dithiozone with canine, porcine and bovine islets;Cell Transplant.3 299–306
Shewade Y, Umarani M and Bhonde R R 1999 Large scale isolation of islets by tissue culture of adult mouse pancreas;Transplant. Proc. 31 1721–1723
Song B B, Anderson D J and Schacht J 1997 Protection from gentamycin ototoxicity by iron chelator in guinea pig “inJ. Pharmocol. Exp. Ther. 282 369–377
Stosic G S, Dimitrijevic M and Barlet R 1999 Leflunomide protects mice from multiple low dose streptozotocin (MLDSZ) induced insulitis and Diabetes;Clin. Exp. Immunol. 17 44–50
Thelwaris J A, Hayglass K T, Manchur D, Rector E, Begleiter A and Johnston J B 2000 Functional status of immune system after chronic administration of 2′-deoxyconformycinin BB rats;Histol. Histopathol. 15 21–27
Vijayalakshmi K S, Menon V P and Leelamma S 1992 Role of antibiotics in lipid peroxidation;Indian J. Biochem. Biophys. 29 371–374
Warburton S and James R 1995 Hemocytometer cell counts and viability studies; inCell and tissue culture: Laboratory procedures (eds) A Doyle, J B Griffiths and D G Newell (New York: John Wiley) pp 4B:1.1-l.5
White S A, Swann A, Hughes D P, Contractor H H and London N J 1997 Different treatment regimens to reduce microbial contamination of purified porcine islets of Langerhans;Transplantation 64 770–773
Zugar A, Wolf B Z, El-Sadr W and Rahal P 1986 Pentamycin associated fatal acute pancreatitis;JAMA 25 2382–2385
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Shewade, Y., Tirth, S. & Bhonde, R.R. Pancreatic islet-cell viability, functionality and oxidative status remain unaffected at pharmacological concentrations of commonly used antibioticsin vitro . J Biosci 26, 349–355 (2001). https://doi.org/10.1007/BF02703744
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DOI: https://doi.org/10.1007/BF02703744