Summary
A method based on solid phase extraction and reversed phase LC for quantitation of IPPD (N-(1-methylethyl)-N′-phenyl-1,4-Benzendiamine) and 6PPD (N-(2,3-dimethylpropyl)-N′-phenyl-1,4-Benzendiamine) in the urine of rubber vulcanization workers is described. The method provided a 91.8 +/- 1.45 % mean recovery of IPPD and 93.8 +/- 1.65 % mean recovery of 6PPD from fortified urines in the 10.0 to 250.0 µg l−1 range. Standard curves bracketing this concentration range had linear coefficients greater than 0.9989. The average relative standard deviation for the entire concentration range was 1.93 % for IPPD and 2.3 % for 6PPD. The method has been applied successfully to biomonitoring IPPD in the nanomole range in the urine of rubber vulcanization workers.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
IARC Monographs on the evaluation of the carcinogenic risk of chemicals to humans. “The rubber industry”, vol. 28. ISRC, Lyon, pp. 252 (1982).
BRMA Toxicity and safe handling of rubber chemicals. “BRMA Code of practice”, British Rubber Manufacturers’ Association Ltd. Birmingham (1983).
E. Capodaglio, L. Pozzoli, V. Foa, A. Colombi, C. Sassi, A. Fantuzzi, “Rischi, patologia e prevenzione nell’ industria della gomma”, Acireale, pp. 549–556 (1983).
F. Belliardo, I. Pavan, J. Liquid Chrom.4, 279 (1981).
G. Scansetti, E. Buglione, M. M. Massiccio, D. Greghi, L. Inglese, W. Pisani, I. Pavan, Int. Arch. Occup. Environ. Health, in press (1987).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pavan, I., Belliardo, F., Buglione, E. et al. Biomonitoring of workers exposed to aromatic amines in rubber vulcanization. Chromatographia 24, 651–654 (1987). https://doi.org/10.1007/BF02688561
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02688561