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Preparative HPLC for analysis of mineralocorticoids in human plasma

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Summary

A method has been developed to measure simultaneously in human plasma the mineralocorticoids and glucocorticoids involved in mineralocorticoid excess.

Briefly: plasma extracts were chromatographied in a fully-automated procedure on a lichrospher-diol column using a hexane: isoproanol: trithylamine gradient. Eluates were collected on a preprogrammed fraction collector. Final quantification was achieved by RIA on the selected fractions.

This procedure allowed: 1) the separation of each measured steroid from plasma contaminants; 2) precise collection; 3) quantification of mineralocorticoids with a precision and sensitivity equivalent to that of direct methods; and 4) improved specificity.

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Authors and Affiliations

  1. Chimie Médicale, Hôpital Saint-Pierre, Université Libre de Bruxelles, rue Haute, 322, 1000, Bruxelles, Belgium

    C. Lejeune-Lenain, S. Kina & D. Bosson

Authors
  1. C. Lejeune-Lenain
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  2. S. Kina
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  3. D. Bosson
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Lejeune-Lenain, C., Kina, S. & Bosson, D. Preparative HPLC for analysis of mineralocorticoids in human plasma. Chromatographia 24, 333–338 (1987). https://doi.org/10.1007/BF02688501

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  • DOI: https://doi.org/10.1007/BF02688501

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