Skip to main content
Springer Nature Link
Log in

A simple and efficient method for isolating RNA from pine trees

  • Commentary
  • Published:
Plant Molecular Biology Reporter Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Abbreviations

CTAB:

hexadecyltrimethylammonium bromide

IAA:

isoamyl alcohol

SSTE:

defined inMaterials and Methods

References

  • Baker, S. S., C. L. Rugh, and J. C. Kamalay. 1990. RNA and DNA isolation from recalcitrant plant tissues. Biotechniques 9(3):268–272.

    PubMed  CAS  Google Scholar 

  • Fang, G., S. Hammar, and R. Grumet. 1992. A quick and inexpensive method for removing polysaccharides from plant genomic DNA. Biotechniques 13:52–55.

    PubMed  CAS  Google Scholar 

  • Katterman, F.R.H., and V.I. Shattuck. 1983. An effective method of DNA isolation from the mature leaves ofGossypium species that contain large amounts of phenolic terpenoids and tannins. Prep. Biochem. 13:347–359.

    Article  PubMed  CAS  Google Scholar 

  • Sambrook, J., E. F. Fritsch, and T. Maniatis, 1989.Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Press, Cold Spring Harbor, New York.

    Google Scholar 

  • Schneiderbauer, A., H. Sandermann Jr., and D. Ernst. 1991. Isolation of functional RNA from plant tissues rich in phenolic compounds. Anal. Biochemistry 197:91–95.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Forest Science, Texas A&M University, 77843-2135, College Station, Texas, USA

    Shujun Chang, Jeff Puryear & John Cairney

Authors
  1. Shujun Chang
    View author publications

    You can also search for this author inPubMed Google Scholar

  2. Jeff Puryear
    View author publications

    You can also search for this author inPubMed Google Scholar

  3. John Cairney
    View author publications

    You can also search for this author inPubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chang, S., Puryear, J. & Cairney, J. A simple and efficient method for isolating RNA from pine trees. Plant Mol Biol Rep 11, 113–116 (1993). https://doi.org/10.1007/BF02670468

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02670468

Key words