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Selective elimination of fibroblasts from cultures of normal human melanocytes

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Summary

The main obstacle to establishing pure normal human melanocytes in vitro is contamination of the cultures by fibroblasts. The obstacle can be overcome by selective destruction of fibroblasts with geneticin (G418 sulfate). Treatment of mixed cultures with this drug at a concentration of 100 μg/ml for two days results in pure cultures of normal human melanocytes.

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References

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Author information

Authors and Affiliations

  1. Department of Dermatology, Yale University School of Medicine, 333 Cedar Street, 06510, New Haven, Connecticut

    Ruth Halaban & Francis D. Alfano

Authors
  1. Ruth Halaban
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  2. Francis D. Alfano
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Additional information

editor A. B. Lerner

This work was supported by National Institutes of Health Grant 5 RO1 CA04679, to A. B. Lerner. The authors are grateful to Dr. A. B. Lerner for his support, to Dr. G. Moellmann for editing and Mrs. C. Kocher for typing the manuscript.

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Halaban, R., Alfano, F.D. Selective elimination of fibroblasts from cultures of normal human melanocytes. In Vitro 20, 447–450 (1984). https://doi.org/10.1007/BF02619590

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  • DOI: https://doi.org/10.1007/BF02619590

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