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Liposomes as fatty acids carriers in isolated rat liver: effect on energy metabolism and on isolated mitochondria activity

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Abstract

The effects of fatty acids (FA)-carrier, egg-lecithin liposomes (LIPO) as alternative to BSA, on ATP, glycogen and glucose contents in isolated perfused liver of fed rats were non-invasively studied using31P/13C nuclear magnetic resonance (NMR). Oxidative phosohorylation was studied in isolated mitochondria from the same liver consecutively to the NMR experiments. ATP content decreased slowly and ATP turnover was similar during the perfusion with saline solution (KHB) or LIPO. However. LIPO induced an enhancement of respiratory control ratio in isolated mitochondria. Tissue glycogen and glucose content decreased when FA (linoleate or linolenate) were perfused with defatted BSA, (3%) or LIPO (600 mg/l) whereas glucose excretion level was unchanged and lactate excretion tended to increase, reflecting changes in the cytosolic redox state and/or an enhancement of glycolysis. Addition of FA (0.5 or 1.5 mM) to LIPO caused a dramatic fall in liver ATP, a mitochondrial uncoupling and an impairment of the phosphorylation activity. Perfusion with FA (1.5 mM) carried by BSA significantly increased the ATP degradation without change of mitochondrial function. Owing to the higher affinity of BSA than LIPO for FA, these latter could be more easily released from complex LIPO-FA, increasing their uncoupling effect. Hence, the FA concentrations have to be largely decreased from the above currently used concentrations to avoids this effect. It will then be possible to minimize the effector action of FA and to study their more specific metabolic function as fuel. It was concluded that LIPO were appropriate carriers to study the different metabolic effects of FA.

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Abbreviations

BSA:

bovine serum albumin

EGTA:

ethylene glycol-bis (β-aminoethylether)N,N,N',N'-tetraacetic acid

FA:

Fatty acids

KHB:

Krebs-Henseleit bicarbonate medium

LIPO:

liposomes

MDPA:

methylene diphosphonic acid

NMR:

nuclear magnetic resonance

NTP:

nucleoside triphosphates

Pi:

inorganic phosphate

TES:

N-tris(hydroxymethyl)methyl-2-aminoethane-sulfonic acid

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Authors and Affiliations

  1. Service de Nutrition et Diabétologie, Hôpital Haut-Lévêque, Avenue Magellan, 33604, Pessac, France

    M. C. Delmas-Beauvieux & H. Gin

  2. RMSB UMR 5536, CNRS-Université Victor Segalen Bordeaux 2 (Case 93), 146 rue Léo Saignat, 33076, Bordeaux Cedex, France

    M. C. Delmas-Beauvieux, N. Leducq, E. Thiaudière, P. Diolez, H. Gin, P. Canioni & J. L. Gallis

Authors
  1. M. C. Delmas-Beauvieux
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  2. N. Leducq
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  3. E. Thiaudière
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  4. P. Diolez
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  5. H. Gin
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  6. P. Canioni
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  7. J. L. Gallis
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Delmas-Beauvieux, M.C., Leducq, N., Thiaudière, E. et al. Liposomes as fatty acids carriers in isolated rat liver: effect on energy metabolism and on isolated mitochondria activity. MAGMA 10, 43–51 (2000). https://doi.org/10.1007/BF02613111

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  • DOI: https://doi.org/10.1007/BF02613111

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