Abstract
Differences betweenSpiroplasma citri isolates were detected by one-dimensional electrophoresis of proteins on gradient polyacrylamide slab gels. Two-dimensional protein maps (electrofocusing followed by electrophoresis) showed a highly characteristic pattern for allS. citri isolates examined. Coanalysis of mixed protein samples from pairs ofS. citri strains revealed more than 150 comigrating proteins common to allS. citri isolates, but also a number of noncomigrating proteins. Some noncomigrating proteins were present in one isolate but not in another, while other proteins whose migrational properties were only slightly different from one isolate to the other (homologous proteins), were present in more than one isolate.S. citri isolates had many common and only a few homologous proteins. Comparisons ofS. citri with the corn stunt spiroplasma revealed few common proteins and a large number of homologous proteins. When comparingS. citri and the suckling mouse cataract spiroplasma, few common and homologous proteins were apparent. However, several of these common proteins were also shared by the corn stunt spiroplasma, suggesting that they may well represent genus-specific proteins. The data also offer additional evidence that the suckling mouse cataract spiroplasma differs significantly fromS. citri and corn stunt spiroplasmas and probably deserves a separate species designation.
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Literature Cited
Bonner, W. M., Laskey, R. A. 1974. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry46:83–88.
Bové, J. M., Vignault, J. C., Garnier, M., Saillard, C., Garcia-Jurado, O., Bové, C., Nhami, A. 1978. Mise en évidence deSpiroplasma citri, l'agent causal de la maladie du “stubborn” des agrumes, dans des pervenches (Vinca rosea) ornementales de la ville de Rabat, Maroc. Comptes Rendus de l'Académie des Sciences. D286:57–59.
Chen, T. A., Liao, C. H. 1975. Corn stunt spiroplasma: Isolation, cultivation and proof of pathogenicity. Science188:1015–1017.
Daniels, M. J., Meddins, B. M. 1973. Polyacrylamide gel electrophoresis of mycoplasma proteins in sodium-dodecyl-sulfate. Journal of General Microbiology76:239–242.
Giannotti, M. J. 1974. Cultures de mollicutes à partir de plantes atteintes de jaunisse et d'insectes vecteurs. Les mycoplasmes. Colloques de l'Institut National de la Santé et de la Recherche Médicale, Bordeaux33:99–106.
Laemli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature227:680–685.
Mouches, C., Duthil, P., Vignault, J. C., Saillard, C., Bové, J. M. 1978. Mapping of spiroplasma proteins by high resolution one and two dimensional polyacrylamide gel analysis. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene, Abt. I Orig., Reihe A241:221.
O'Farrell, P. 1975. High resolution two dimensional electrophoresis of proteins. Journal of Biological Chemistry250:4007–4021.
Razin, S. 1968. Mycoplasma taxonomy studied by electrophoresis of cell proteins. Journal of Bacteriology96:687–694.
Razin, S., Rottem, S. 1967. Identification ofMycoplasma and other micro-organisms by polyacrylamide gel electrophoresis of cell proteins. Journal of Bacteriology94:1807–1810.
Rodwell, A. W., Archer, D. B., Peterson, J. E., Rodwell, E. S. 1978. The protein of mycoplasmas studied by two-dimensional gel electrophoresis. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene, Abt. 1 Orig., Reihe A241:178–179.
Tully, J. G., Whitcomb, R. F., Clark, H. F., Williamson, D. L. 1977. Pathogenic mycoplasmas: Cultivation and vertebrate pathogenicity of a new spiroplasma. Science195:892–894.
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Mouches, C., Vignault, J.C., Tully, J.G. et al. Characterization of Spiroplasmas by one- and two-dimensional protein analysis on polyacrylamide slab gels. Current Microbiology 2, 69–74 (1979). https://doi.org/10.1007/BF02603058
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DOI: https://doi.org/10.1007/BF02603058