Summary
We previously reported that co-transplantation of hepatocytes and islets of Langerhans in the spleen reduces the mortality rate after 90% hepatectomy in the rat. In the present study, we examined whether implantation of isolated hepatocytes into the pancreas is as efficient as co-transplantation of hepatocytes and islets in this respect. We found that cotransplantation of hepatocytes and islets into the renal subcapsule reduced the long-term (30-day) mortality rate after 90% hepatectomy from 100% to 36% (P<0.05) when performed the day before the hepatectomy. In contrast, there was no significant reduction of mortality when the hepatocytes were transplanted into the pancreas. The results show that a close proximity of transplanted pancreatic islets to the hepatocytes is of critical importance for the improved survival in acute liver failure.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Baumgartner D, Laplaute-O'Neill PM, Sutherland DER, Najarian JS (1983) Effects of intrasplenic injection of hepatocytes, hepatocyte fragments and hepatocyte culture supernatants on D-galactosamine-induced liver failure in rats. Eur Surg Res 15:129–135
Bruss ML, Black AL (1978) Enzymatic microdetermination of glycogen. Anal Biochem 84:309–311
Bucher NLR, Swanfield MN (1975) Regulation of hepatic regeneration in rats by synergistic action of insulin and glucagen. Proc Natl Acad Sci 72:1157–1159
Cuervas-Mons V, Cienfuegos JA, Maganto P, Golitsin A, Eroles G, Castillo-Olivares J, Segovia de Arana JM (1984) Time-related efficacy of liver cell isograft in fulminant hepatic failure in rats. Transplantation 38:23–25
Demetriou AA, Reisiner A, Sanchez J, Levensson SM, Moscioni AD, Chowdhury JR (1988) Transplantation of microcarrier-attached hepatocytes into 90% partially hepatectomized rats. Hepatology 8:1006–1009
Emond J, Capron-Laudereau M, Meriggi F, Beruou J, Reynes M, Hossin D (1989) Extent of hepatectomy in the rats: evaluation of basal condition and effect of therapy. Eur Surg Res 12:251–259
Flye MW, Xinsheng L, Semuel Y (1987) Reversal of ischemic liver failure by intrasplenic liver cells transplantation. J Pediatr Surg 22:1212–1215
Innes GK, Fuller BJ, Hobbs KEF (1988) Functional testing of hepatocytes following their recovery from cryopreservation. Cryobiology 25:23–30
Lacy PE, Kostianovsky M (1967) Method for the isolation of intact islets of Langerhans from the rat pancreas. Diabetes 16:35–39
Lopez MP, Gomez-Lechon MJ, Castell JV (1984) Glycogen synthesis in serum-free cultured hepatocytes in response to insulin and dexamethasone. In Vitro 20:923–931
Shu-ren W, Guyrenand A, Infante JL, Gatala D, Infante R (1985) Isolation of rat hepatocytes with EDTA and their metabolic function in primary culture. In Vitro 21:526–530
Suchiya MT, Yoshikawa H, Itakura M, Yamashita K (1990) Increased de novo purine synthesis by insulin through selective enzyme induction in primary cultured rat hepatocytes. Am J Physiol 258:C841-C848
Xiangdong W, Ar'Rajab A, Ahrén B, Andersson R, Bengmark S (1991) Improvement of the effects of intrasplenic transplantation of hepatocytes after 90% hepatectomy in the rat by contransplantation with pancreatic islets. Transplantation 52 (in press)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Xiangdong, W., Ar'Rajab, A., Ahrén, B. et al. The effect of pancreatic islets on transplanted hepatocytes in the treatment of acute liver failure in rats. Res. Exp. Med. 191, 429–435 (1991). https://doi.org/10.1007/BF02576698
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02576698