Summary
Diurnal changes in the neurotransmitter receptors are important for studying the receptor function in neurophysiology. The purpose of this study is to gain an insight into the regulatory mechanisms of the diurnal variation of amylase secretion. Rat salivary amylase levels showed a diurnal variation with two peaks, a marked peak at 13 h and a lesser peak at 21 h. This increase in salivary amylase levels was completely inhibited by pretreatment of rats with the β-adrenergic antagonist propranolol, but not by the α-adrenergic antagonist phentolamine. Amylase level in parotid tissue homogenate also showed a diurnal change, but there was only one peak, at 13 h. The number of maximal binding sites (Bmax) for [3H]dihydroalprenolol (DHA) in parotid membranes showed a diurnal variation with two marked peaks at 13 and 21 h, but the affinity of parotid β-adrenoceptors for agonists or antagonists did not show any diurnal changes. Phosphorylation of nuclear non-histone proteins in the rat parotid gland showed diurnal variation with two marked peaks at 13 and 21 h. These results indicate that a diurnal variation in the number of rat parotid β-adrenoceptors, which is presumably regulated by gene expression, is coupled with a change in salivary amylase secretion.
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References
Bernfeld P (1955) Amylase α and β. In: Colowick SP, Kaplan NO (eds) Methods in enzymology. Academic Press, New York
Bethea CL, Neill JD (1979) Prolactin secretion after cervical stimulation of rats maintained in constant dark or constant light. Endocrinology 104:870–876
Burbach JPH, Liu B, Voorhuis TAM, Van Tol HHM (1988) Diurnal variation in vasopressin and oxytocin messenger RNAs in hypothalamic nuclei of the rat. Mol Brain Res 4:157–160
Clarke CF, Fogelman AM, Edwards PA (1984) Diurnal rhythm of rat liver mRNAs encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase. J Biol Chem 259:10439–10447
Cugini P, Lucia P, Tomassini R, Centanni M, Salandi E, Scavo D (1982) Circadian rhythms of plasma renin, aldosterone and cortisol. Effect of beta-adrenergic blockade by propranolol in patients with recent, advanced and complicated forms of essential hypertension. Chronobiologia 9:229–235
Gonzalez-Brito A, Reiter RJ, Menendez-Pelaez A, Guerrero JM, Santan C, Jones DJ (1988) Darkness-induced changes in noradrenergic input determine the 24 hour variation in beta-adrenergic receptor density in the rat pineal gland: in vivo physiological and pharmacological evidence. Life Sci 43:707–714
Hata F, Ishida H, Kagawa K, Kondo E, Kondo S, Noguchi Y (1983) β-Adrenoceptor alterations coupled with secretory response in rat parotid tissue. J Physiol (Lond) 341:185–196
Ishida H, Ahmed K (1973) Studies on phosphoproteins of submandibular gland nuclei isolated from isoproterenol-treated rats. Exp Cell Res 78:31–40
Ishida H, Ahmed K, (1974) Studies on chromatin-associated protein phosphokinase of submandibular gland from isoproterenol-treated rats. Exp Cell Res 84:127–136
Jarrousse C, Lardeux B, Bourdel G, Girard-Globa A, Rosselin G (1980) Portal insulin and glucagon in rats fed proteins as a meal: immediate variations and circadian modulations. J Nutr 110:1764–1773
Kafka MS, Wirz-Justice A, Naber D (1981) Circadian and seasonal rhythms in α- and β-adrenergic receptors in the rat brain. Brain Res 207:409–419
Kafka MS, Wirz-Justice A, Naber D, Moore RY, Benedito MA (1983) Circadian rhythms in rat brain neurotransmitter receptors. Fed Proc 42:2796–2801
Kafka MS, Benedito MA, Roth RH, Steele LK, Wolfe WW, Catravas GN (1986) Circadian rhythms in catecholamine metabolites and cyclic nucleotide production. Chronobiol Int 3:101–115
Kanno T, Saito A, Ishikawa K, Habara Y, Abe Y, Mizoguchi J (1979) Biological rhythms and their control mechanism. In: Suda M, Hayaishi O, Nakagawa H (eds) Elsevier/North-Holland Biochemical Press, Amsterdam, pp 273–279
Kräuchi K, Wirz-Justice A, Morimasa T, Willener R, Feer H (1984) Hypothalamic α2− and β-Adrenoceptor rhythms are correlated with circadian feeding: evidence from chronic methamphetamine treatment and withdrawal. Brain Res 321:83–90
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275
Miki K, Ishida H, Yamamoto I (1976) Effect of cytarabine and bleomycin on the phosphorylation of nuclear acidic protein. FEBS Lett 69:249–251
Murakami M, Greer MA, Hjulstad S, Greer SE, Tanaka K (1989) Evidence that rat pineal thyroxine 5′-deiodinase in primarily stimulated by β- and not α-adrenergic agonists and that its adrenergic-stimulated and spontaneous rhythmic nocturnal rise require RNA and protein synthesis (42849). Proc Soc Exp Biol Med 190:190–194
Scatchard G (1949) The alterations of proteins for small molecules and ions. Ann NY Acad Sci 51:660–672
Schramm M, Danon D (1961) The mechanism of enzyme secretion by the cell. I. Storage of amylase in the zymogen granules of the rat-parotis gland. Biochim Biophys Acta 50: 102–112
Sibley DR, Lefkowitz RJ (1985) Molecular mechanisms of receptor desensitization using the β-adrenergic receptor-coupled adenylate cyclase system as a model. Nature 317:124–129
Sreebny LM, Johnson DA, Robinovitch MR (1971) Functional regulation of protein synthesis in the rat parotid gland. J Biol Chem 246:3879–3884
Stein GS, Spelsberg TC, Kleinsmith LJ (1974) Nonhistone chromosomal proteins and gene regulation. Science 183:817–824
Yamamoto H, Nagai K, Nakagawa H (1987) Role of SCN in daily rhythms of plasma glucose, FFA, insulin and glucagon. Chronobiol Int 4:483–491
Wirz-Justice A (1987) Circadian rhythms in mammalian neurotransmitter receptors. Prog Neurobiol 29:219–259
Wurtman RJ, Axelrod J (1966) A 24-hour rhythm in the content of norepinephrine in the pineal and salivary glands of the rat. Life Sci 5:665–669
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Ishikawa, Y., Amano, I., Chen, C. et al. Diurnal variation of amylase secretion is coupled with alterations of β-adrenoceptors in the rat parotid gland. Res. Exp. Med. 192, 231–240 (1992). https://doi.org/10.1007/BF02576279
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DOI: https://doi.org/10.1007/BF02576279