Abstract
Phytochromes are chromoproteins which mediate several light responses in plants. Phytochrome proteins are encoded by a gene family which is currently being characterized in several plant species. Analysis of type-specific mutants of two well-characterized members of the family, PhyA and PhyB, indicates that these proteins have distinct functions. Much remains to be learned about the mechanisms by which the phytochromes carry out their distinct and diverse functions. It is hoped that information concerning the localization of phytochromes, at the whole plant and subcellular levels, will aid in elucidating the mechanism of phytochrome function. This review, which summarizes information about phytochrome distribution, has an emphasis on recent reports in which the molecular species of phytochrome are differentiated. However, classical data are also included and reinterpreted using knowledge of the phytochrome family.
Abbreviations
- GUS:
-
β-glucuronidase
- PhyA:
-
phytochrome A
- Phy B:
-
phytochrome B
- Pr:
-
red light-absorbing form of phytochrome
- Pfr:
-
far-red light-absorbing form of phytochrome
References
Abe, H., Takio, K., Titani, K. andFuruya, M. 1989. Amino-terminal amino acid sequences of pea phytochrome II fragments obtained by limited proteolysis. Plant Cell Physiol.30: 1089–1097.
Adam, E., Kozma Bognar, L., Kolar, C., Schäfer, E. andNagy, F. 1996. The tissue-specific expession of a tobacco phytochrome B gene. Plant Physiol.110: 1081–1088.
Adam, E., Szell, M., Szekeres, M., Schäfer, E. andNagy, F. 1994. The developmental and tissue-specific expression of tobacco phytochrome-A genes. Plant J.6: 283–293.
Black, M. andShuttleworth, J.E. 1974. The role of the cotyledons in the photocontrol of hypocotyl extension inCucumis sativus L. Planta117: 57–66.
Borthwick, H.A., Hendricks, S.B., andParker, M.W. 1952a. The reaction controlling floral initiation. Proc. Natl. Acad. Sci. USA38: 929–934.
Borthwick, H.A., Hendricks, S.B., Parker, M.W., Toole, E.H. andToole, V.K. 1952b. A reversible photoreaction controlling seed germination. Proc. Natl. Acad. Sci. USA38: 662–666.
Briggs, W.R. andSiegelman, H.W. 1965. Distribution of phytochrome in etiolated seedlings. Plant Physiol.40: 934–941.
Butler, W.L., Norris, K.H., Siegelman, H.W. andHendricks, S.B. 1959. Detection, assay, and preliminary purification of the pigment controlling photoresponsive development of plants. Proc. Natl. Acad. Sci. USA45: 1703–1708.
Clack, T., Mathews, S. andSharrock, R.A. 1994. The phytochrome apoprotein family inArabidopsis is encoded by five genes: The sequences and expression ofPHYD andPHYE. Plant Mol. Biol.25: 413–427.
Coleman, R.A. andPratt, L.H. 1974. Electron microscopic localization of phytochrome in plants using an indirect antibody labeling method. J. Histochem. Cytochem.22: 1039–1047.
Datta, N., Chen, Y.-R. andRoux, S.J. 1985. Phytochrome and calcium stimulation of protein phosphorylation in isolated pea nuclei. Biochem. Biophys. Res. Commun.128: 1403–1408.
Dehesh, K., Tepperman, J., Christensen, A.H. andQuail, P.H. 1991.phyB is evolutionarily conserved and constitutively expressed in rice seedling shoots. Mol. Gen. Genet.225: 305–313.
Duke, S.O. andWickliff, J.L. 1969.Zea shoot development in response to red light interruption of the darkgrowth period. I. Inhibition of the first internode elongation. Plant Physiol.44: 1027–1030.
Evans A. andSmith, H. 1976. Spectrophotemetric evidence for the presence of phytochrome in the envelope membranes of barley etioplasts. Nature259: 323–325.
Fondeville, J.C., Borthwick, H.A. andHendricks, S.B. 1966. Leaflet movement ofMimosa pudica L. Indicative of phytochrome action. Planta69: 357–364.
Furuya, M. 1993. Phytochromes-Their molecular species, gene families, and functions. Annu. Rev. Plant Physiol. Plant Mol. Biol.44: 617–645.
Furuya, M. andSchäfer, E. 1996. Photoperception and signalling of induction reactions by different phytochromes. Trends Plant Sci.1: 301–307.
Gaba, V. andBlack, M. 1983. The control of cell growth by light.In W. Shropshire, Jr. and H. Mohr ed., Encyclopedia of Plant Physiology New Ser., vol. 16A, Springer-Verlag, Berlin, pp. 358–400.
Haupt, W., Mörtel, G. andWinkelnkemper, I. 1969. Demonstration of different dichroic orientation of phytochrome Pr and Pfr. Planta88: 183–186.
Hershey, H.O., Barker, R.F., Idler, K.B., Lissemore, J.L. andQuail, P.H. 1985. Analysis of cloned cDNA and genomic sequences for phytochrome: Complete amino acid sequences for two gene products expresed in etiolatedAvena. Nucleic Acids Res.13: 8543–8559.
Heyer, A. andGatz, C. 1992a. Isolation and characterization of a cDNA-clone coding for potato type-A phytochrome. Plant Mol. Biol.18: 535–544.
Heyer, A. andGatz, C. 1992b. Isolation and characterization of a cDNA-clone coding for potato type-B phytochrome. Plant Mol. Biol.20: 589–600.
Jabben, M. andHolmes, M.G. 1983. Phytochrome in light-grown plants.In W. Shropshire, Jr. and H. Mohr ed., Encyclopedia of Plant Physiology New Ser., vol. 16A, Springer-Verlag, Berlin. pp. 704–722.
Johnson, E., Bradley, M., Harberd, N.P. andWhitelam, G.C. 1994. Photoresponses of light-grown phyA mutants ofArabidopsis-Phytochrome A is required for the perception of daylength extensions. Plant Physiol.105: 141–149.
Johnson, E.M., Pao, L.I. andFeldman, L.J. 1991. Regulation of phytochrome message abundance in root caps of maize. Plant Physiol.95: 544–550.
Jordan, E.T., Hatfield, P.M., Hondred, D., Talon, M., Zeevaart, J.A.D. andVierstra, R.D. 1995. Phytochrome A overexpression in transgenic tobacco-Correlation of dwarf phenotype with high concentrations of phytochrome in vascular tissue and attenuated gibberellin levels. Plant Physiol.107: 797–805.
Kendrick, R.E. andKronenberg, G.H.M. ed. 1994. Photomorphogenesis in Plants. Kluwer Academic Publishers, Dordrecht.
Komeda, Y., Yamashita, H., Sato, N., Tsukaya, H. andNaito, S. 1991. Regulated expression of a genefusion product derived from the gene for phytochromel fromPisum sativum and theuidA gene fromE. Coli in transgenicPetunia-hybrida. Plant Cell Physiol.32: 737–743.
Konomi, K., Abe, H. andM. Furuya 1987. Changes in the content of phytochrome I and II apoproteins in embryonic axes of pea seeds during imbibition. Plant Cell Physiol.28: 1443–1451.
Kunkel, T., Tomizawa, K.-I., Kern, R., Furuya, M., Chua, N.-H. andSchäfer, E. 1993.In vitro formation of a photoreversible adduct of phycocyanobilin and tobacco apophytochrome B. Eur. J. Biochem.215: 587–594.
López-Juez, E., Nagatani, A., Tomizawa, K.I., Deak, M., Kern, R., Kendrick, R.E. andFuruya, M. 1992. The cucumber long hypocotyl mutant lacks a light-stable PHYB-like phytochrome. Plant Cell4: 241–251.
Mackenzie, J.M., Briggs, W.R. andPratt, L.H. 1978. Intracellular phytochrome distribution as a function of its molecular form and of its destruction. Am. J. Bot.65: 671–676.
Mackenzie, J.M., Coleman, J.M., Briggs, W.R. andPratt, L.H. 1975. Reversible redistribution of phytochrome within the cell upon conversion to its physiologically active form. Proc. Natl. Acad. Sci. USA72: 799–803.
Manabe, K. andFuruya, M. 1975. Distribution and non-photochemical transformation of phytochrome in subcellular fractions fromPisum epicotyls. Plant Physiol.56: 772–775.
Mancinelli, A.L. 1994. The physiology of phytochrome action.In R.E. Kendrick and G.H.M. Kronenberg ed., Photomorphogenesis in Plants, Kluwer Academic Publishers, Dordrecht. pp. 211–269.
Mandoli, D.F. andBriggs, W.R. 1988. The photoperceptive sites and the function of tissue light-piping in photomorphogenesis of etiolated oat seedlings. Plant Cell Environ.5: 137–145.
McCurdy, D.W. andPratt L.H. 1986. Immunogold electron microscopy of phytochrome in Avena: Identification of intracellular sites responsible for phytochrome sequestering and enhanced pelletability. J. Cell Biol.103: 2541–2550.
Nagatani, A., Chory, J. andFuruya, M. 1991. Phytochrome B is not detectable in thehy3 mutant ofArabidopsis, which is deficient in responding to end-of-day far-red light treatments. Plant Cell Physiol.32: 1119–1122.
Nagatani, A., Jenkins, G.I. andFuruya, M. 1988. Non-specific association of phytochrome to nuclei during isolation from dark-grown pea (Pisum sativum cv. Alaska) plumules. Plant Cell Physiol.29: 1141–1145.
Nagatani, A., Lumsden, P.J., Konomi, K. andAbe, H. 1987. Application of monoclonal antibodies to phytochrome studies.In M. Furuya ed., Phytochrome and Photoregulation in Plants Academic Press, Tokyo. pp. 95–114.
Nagatani, A., Reed, J.W. andChory, J. 1993. Isolation and initial characterization ofArabidopsis mutants that are deficient in phytochrome A. Plant Physiol.102: 269–277.
Neuhaus, G., Bowler, C., Kern, R. andChua, N.H. 1993. Calcium/calmodulin-dependent and calcium/calniodulin-independent phytochrome signal transduction pathways. Cell73: 937–952.
Nick, P., Ehman, B., Furuya, M. andSchäfer, E. 1993. Cell communication, stochastic cell responses, and anthocyanin pattern in mustard cotyledons. Plant Cell5: 541–552.
Parks, B.M. andQuail, P.H. 1993.hy8, a new class of Arabidopsis long hypocotyl mutants deficient in functional phytochrome A. Plant Cell,5: 39–48.
Powell, R.D. andMorgan, P.W. 1980. Opening of the hypocotyl hook in seedlings as influenced by light and adjacent tissues. Planta148: 188–191.
Pratt, L.H. 1983. Assay of photomorphogenic photoreceptors.In W. Shropshire, Jr. and H. Mohr ed., Encyclopedia of Plant Physiology New Ser., vol. 16A, Springer-Verlag, Berlin. pp. 152–177.
Pratt, L.H. 1994. Distribution and localization of phytochrome within the plant.In R.E. Kendrick and, G.H.M. Kronenberg ed., Photomorphogenesis in Plants, Kluwer Academic Publishers, Dordrecht. pp. 163–185.
Pratt, L.H. andColeman, R.A. 1971. Immunocytochemical localization of phytochrome. Proc. Natl. Acad. Sci. USA68: 2431–2435.
Quail, P.H. 1983. Rapid action of phytochrome in photomorphogenesis.In W. Shropshire, Jr. and H. Mohr ed., Encyclopedia of Plant Physiology New Ser., vol. 16A, Springer-Verlag, Berlin, pp. 178–212.
Raikhel, N. 1992. Nuclear targeting in plants. Plant Physiol.100: 1627–1632.
Reed, J.W., Nagatani, A., Elich, T.D., Fagan, M. andChory, J. 1994. Phytochrome A and phytochrome B have overlapping but distinct functions in Arabidopsis development. Plant Physiol.104: 1139–1149.
Reed, J.W., Nagpal, P., Poole, D.S., Furuya, M. andChory, J. 1993. Mutations in the gene for the red far-red light receptor phytochrome B alter cell elongation and physiological responses throughoutArabidopsis development. Plant Cell,5: 147–157.
Romeo, L.C., Sommer, D., Gotor, C. andSong, P.-S. 1991. Protein phosphorylation in isolated nuclei from etiolatedAvena seedlings. Effects of red/far-red light and cholera toxin. FEBS Lett.282: 347–350.
Sage, L.C. 1992. Pigment of the Imagination-A History of Phytochrome Research. Academic Press, San Diego.
Sakamoto, K. andNagatani, A. 1996. Nuclear localization activity of phytochrome B. Plant J.10: 859–868.
Sharrock, R.A. andQuail, P.H. 1989. Novel phytochrome sequences inArabidopsis thaliana: Structure, evolution, and differential expression of a plant regulatory photoreceptor family. Genes Dev.3: 1745–1757.
Shinomura, T., Nagatani, A., Chory, J. andFuruya, M. 1994. The induction of seed germination inArabidopsis thaliana is regulated principally by phytochrome B and secondarily by phytochrome A. Plant Physiol.104: 363–371.
Shinomura, T., Nagatani, A., Hanzawa, H., Kubota, M., Watanabe, M. andFuruya, M. 1996. Action spectra for phytochrome A- and B-specific photoinduction of seed germination inArabidopsis thaliana. Proc. Natl. Acad. Sci. USA93: 8129–8133.
Shropshire, W., Jr. andMohr, H. (ed.) 1983. Photomorphogenesis, Encyclopedia of Plant Physiology, New Ser., vol. 16, Springer-Verlag, Berlin.
Somers, D.E. andQuail, P.H. 1995. Temporal and spatial expression patterns ofPHYA andPHYB genes in Arabidopsis. Plant J.7: 413–427.
Somers, D.E., Sharrock, R.A., Tepperman, J.M. andQuail, P.H. 1991. Thehy3 long hypocotyl mutant of Arabidopsis is deficient in phytochrome-B. Plant Cell3: 1263–1274.
Speth, V., Otto, V. andSchäfer, E. 1986. Intracellular localization of phytochrome in oat coleoptiles by electron microscopy. Planta168: 299–304.
Terry, M.J. andKendrick, R.E. 1996. Theaurea andyellow-green-2 mutants of tomato are deficient in phytochrome chromophore synthesis. J. Biol. Chem.271: 21681–21686.
Vince-Prue, D. 1983. Photomorphogenesis and flowering.In W. Shropshire, Jr. and H. Mohr ed., Encyclopedia of Plant Physiology New Ser., vol. 16A, Springer-Verlag, Berlin. pp. 457–490.
Wada, M., Grolig, F. andHaupt, W. 1993. Light-oriented chloroplast positioning-contribution to progress in photobiology. J. Photochem. Photobiol. B-Biol.17: 3–25.
Wada, M. andKadota, A. 1989. Photomorphogenesis in lower green plants. Annu. Rev. Plant Physiol. Plant Mol. Biol.40: 169–191.
Wada, M., Kadota, A. andFuruya, M. 1981. Intracellular photoreceptive site for polarotropism in protonema of the fernAdiantum capillus-veneris L. Plant Cell Physiol.22: 1481–1488.
Wada, M., Kadota, A. andFuruya, M. 1983. Intracellular localization and dichroic orientation of phytochrome in plasma membrane and/or ectoplasm of a centrifuged protonema of fernAdiantum. Plant Cell Physiol.24: 1441–1447.
Whitelam, G.C., Johnson, E., Peng, J.R., Carol, P., Anderson, M.L., Cowl, J.S. andHarberd, N.P. 1993. Phytochrome-A null mutants of Arabidopsis display a wild-type phenotype in white light. Plant Cell5: 757–768.
Williamson, F.A., J., M.D. andJaffe, M.J. 1975. Association of phytochrome with rough-surfaced endoplasmic reticulum fractions from soybean hypocotyls. Plant Physiol.56: 738–743.
Yanovsky, M.J., Casal, J.J. andWhitelam G.C. 1995. Phytochrome A, phytochrome B and HY4 are involved in hypocotyl growth responses to natural radiation inArabidopsis: Weak de-etiolation of thephyA mutant under dense canopies. Plant Cell Environ.18: 788–794.
Yu, R. 1975. Characterization of the phytochromecontaining particles obtained by glutaraldehyde pre-fixation of maize coleoptiles. J. Exp. Bot.26: 808–822.
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Nagatani, A. Spatial distribution of phytochromes. J. Plant Res. 110, 123–130 (1997). https://doi.org/10.1007/BF02506851
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DOI: https://doi.org/10.1007/BF02506851