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Sensitive determination of styrene and related compounds in human body fluids by headspace capillary gas chromatography with cryogenic oven trapping

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Summary

A simple and sensitive method is presented for determination of styrene, toluene, ethylbenzene, isopropylbenzene andn-propylbenzene in human body fluids by capillary gas chromatography (GC) with cryogenic oven trapping. After heating a blood or urine sample containing each compound andp-diethylbenzene (internal standard, IS) in a 7.0-mL vial at 60°C for 20 min, 5 mL of headspace vapor was drawn into a glass syringe and injected into a GC. All vapor was introduced into an Rtx-Volatile middle bore capillary column in splitless mode at oven temperature of 20°C to trap entire analytes, and the oven temperature then programmed to 280°C for GC measurements by flame ionization detection. The present conditions gave sharp peaks of each compound and IS, and low background noises for whole blood or urine samples.

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Authors and Affiliations

  1. Department of Legal Medicine, Hamamatsu University School of Medicine, 1-20-1 Handayama, 431-3192, Hamamatsu, Japan

    K. Watanabe-Suzuki, Y. Takeuchi & O. Suzuki

  2. Department of Legal Medicine and Bioethics, Nagoya University Graduate School of Medicine, 466-8550, Nagoya, Japan

    A. Ishii

  3. Department of Legal Medicine, Aichi Medical University, Nagakute-cho, 480-1195, Aichi, Japan

    H. Seno

Authors
  1. K. Watanabe-Suzuki
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  2. A. Ishii
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  3. H. Seno
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  4. Y. Takeuchi
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  5. O. Suzuki
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Watanabe-Suzuki, K., Ishii, A., Seno, H. et al. Sensitive determination of styrene and related compounds in human body fluids by headspace capillary gas chromatography with cryogenic oven trapping. Chromatographia 54, 507–510 (2001). https://doi.org/10.1007/BF02491208

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  • DOI: https://doi.org/10.1007/BF02491208

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