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Evaluation of improved method for determination of rapeseed-mustard FAMEs by GC

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Summary

An efficient and economical GC method for rapid determination of FAMEs in rapeseed-mustard is described. The seeds were transmethylated with acetylchloride, using microwave heating and separation achieved on a 3 m column packed with a mixture of 2% SP-2300 and 3% SP-2310 on Chromosorb ‘W’. The method is compared with the conventional heating method and extended efficiently for half-seed analysis. The fatty acid composition of the FAMEs mixtures prepared by both methods was similar with highly significant correlation coefficients (P<0.001).

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Authors and Affiliations

  1. Biotechnology Division, Tata Energy Research Institute, Darbari Seth Block, Habitat Place, Lodi Road, 110 003, New Delhi, India

    N. Kaushik & A. Agnihotri

Authors
  1. N. Kaushik
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  2. A. Agnihotri
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Kaushik, N., Agnihotri, A. Evaluation of improved method for determination of rapeseed-mustard FAMEs by GC. Chromatographia 44, 97–99 (1997). https://doi.org/10.1007/BF02466522

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  • DOI: https://doi.org/10.1007/BF02466522

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