Abstract
Allele and genotype frequencies of the loci LDLR, GYPA, HBGG, D7S8 and GC (PM loci) were investigated in a population sample of 215 unrelated individuals from Asturias (North Spain). Multiplex amplification and simulataneous typing of the five loci was carried out using the polymarker PCR amplification and typing kit. All loci met Hardy-Weinberg expectations. The Asturian sample does not differ significantly from other Caucasians, but significant differences were observed between this population and SW Hispanic, Afro-american and Korean populations.
References
Yamamoto T, Davis CG, Brown MS, Schneider WJ, Casey ML, Goldstein JL, Russell DW (1984) The human LDL receptor: a cysteine-rich protein with multiple Alu sequences in its m-RNA. Cell 39:27–38
Siebert PD, Fukuda M (1987) Molecular cloning of human glycophorin B cDNA: nucleotide sequence and genomic relation-ship to glycophorin A. Proc Natl Acad Sci USA 84:6735–6739
Slightom JL, Blechl AE, Smithies O (1980) Human fetalGγ- andAγ-globin genes: complete nucleotide sequences suggest that DNA can be exchanged between these duplicated genes. Cell 21:627–638
Horn GT, Richards B, Merrill JJ, Klinger KW (1990) Characterization and rapid diagnostic analysis of DNA polymorphisms closely linked to the cystic fibrosis locus. Clin Chem 36:1614–1619
Yang F, Brune JL, Naylor SL, Apples RL, Naberhaus KH (1985) Human group-specific component (Gc) is a member of the albumin family. Proc Natl Acad Sci USA 82:7994–7998
Budowle B, Lindsey JA, DeCou JA, Koons BW, Giusti AM, Comey CT (1995) Validation and population studies of the loci LDLR, GYPA, HBGG, D7S8, and Gc (PM loci), and HLA-DQa using a multiplex amplification and typing procedure. J Forensic Sci 40(1):45–54
Hochmeister MN, Budowle B, Borer UV, Dirnhofer R (1995) A method for the purification and recovery of genomic DNA from an HLA DQA1 amplification product and its subsequent amplification and typing with the AmpliTypeR PM PCR Amplification and Typing Kit. J Forensic Sci 40(4):649–653
Hausmann R, Hantschel M, Lötterle J (1995) Frequencies of the 5 PCR-based genetic markers LDLR, GYPA, HBGG, D7S8, and GC in a North Bavarian population. Int J Legal Med 107:227–228
Woo KM, Budowle B (1995) Korean population data on the PCR-based loci LDLR, GYPA, HBGG, D7S8 HLA-DQA1, and D1S80. J Forensic Sci 40(4):645–648
Hayes JM, Budowle B, Freund M (1995) Arab population data on the PCR-based loci: HLA-DQA1, LDLR, GYPA, HBGG, D7S8, Gc, and D1S80. J Forensic Sci 40(5):888–892
Espinheira R, Ribeiro T, Geada H, Reys L (1994) Polymarker and HLA DQA1 genetic markers in forensic casework. In: Mangin P, Ludes B (eds) Acta Medicinae Legalis, Springer, Berlin Heidelberg New York, pp 37–38
García O, Martín P, Albarrán C, Alonso A (1994) Allele frequencies of HLA-DQA1, LDLR, GYPA, HBGG, D7S8 and Gc in the resident population of the Basque Country. In: Mangin P, Ludes B (eds) Acta Medicinae Legalis Springer, Berlin Heidelberg New York, pp 40–43
Pinheiro MF, Pontes ML, Pinto da Costa J (1994) Use of the AmplyType PM coamplification system on forensic analysis. In: Mangin P, Ludes B (eds) Acta Medicinae Legalis. Springer, Berlin Heidelberg New York, pp 81–82
Rodriguez-Calvo MS, Bellas S, Souto L, Vide C, Valverde E, Carracedo A (1996) Population data on the loci LDLR, GYPA, HBGG, D7S8 and GC in three Southwest European populations. J Forensic Sci 41(2):291–296
Nakajima T, Matsuki T, Ohkawara H, Nara M, Furukawa K, Kishi K (1996) Evaluation of 7 DNA markers (D1S80, HLA-DQa, LDLR, GYPA, HBGG, D7S8 and GC) in a Japanese population. Int J Legal Med 109:47–48
Walsh PS, Metzger DA, Higucchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10:506–513
Budowle B, Monson KL, Chakraborty R (1996) Estimating minimum allele frequencies for DNA profile frequency estimates for PCR-based loci. Int J Legal Med 108:173–176
Fisher RA (1951) Standard calculations for evaluating a blood group system. Heredity. 5:95–102
Nei M, Roychoudhury AK (1974) Sampling variants of heterozygosity and genetic distance. Genetics 76:379–390
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Martínez-Jarreta, B., Abecia, E., Bell, B. et al. Frequencies of the five PCR-based genetic markers LDLR, GYPA, HBGG, D7S8 and GC in the population of Asturias (North Spain). Int J Leg Med 110, 41–43 (1997). https://doi.org/10.1007/BF02441027
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02441027