Abstract
The CD59 (MEM-43) antigen, which probably is a human homologue of mouse Ly-6 antigens, is a broadly expressedM r 18000–25000 human leucocyte surface glycoprotein recognized by monoclonal antibody MEM-43. Ten mouse-human T-lymphocyte hybrids, carrying all mouse chromosomes and a limited number of human chromosomes, were analyzed for expression of CD59 by indirect immunofluorescence and immunoblotting with MEM-43 antibody. Karyotypic analysis of the tested clones showed that the presence of human chromosome 11 correlated with the expression of CD59 in all clones tested. Three other human chromosome 11-encoded antigens, 4F2 (Trop-4), Leu 7 (HNK-1, CD57), and lymphocyte homing receptor, were expressed concordantly with CD59. A more exact localization of the gene for CD59 was obtained by the study of Chinese hamster-human cell hybrids containing short or long arm deletions of human chromosome 11. CD59 segregated with hybrids containing part of the short arm of human chromosome 11, but not with the hybrids containing the long arm. Based on these studies we assign the gene for CD59 to regionP14–p13 of the short arm of chromosome 11.
Similar content being viewed by others
References
Akao, Y., Utsumi, K. R., Naito, K., Ueda, R., Takahashi, T., and Yamada, K.: Gene encoding human p250 T-cell activation antigen maps to human chromosome 11.Somatic Cell Mol Genet 14: 315–320, 1988
Antonucci, T.K., Chapman, V.C., and Meisler M.H.: Linkage of the structural gene for uroporphyrinogen I synthase to markers on mouse chromosome 9 in a cross between feral and inbred mice.Biochem Genet 20: 703–710, 1982
Antonucci, T.K., von Deimling, O. H., Rosenblum, B. B., Skow, L. C., and Meisler, M. H.: Conserved linkage within a 4-cM region of mouse chromosome 9 and human chromosome 11.Genetics 107: 463–475, 1984
Butcher, E. C. and Weissman, I. L.: Cellular, genetic, and evolutionary aspects of lymphocyte interactions with high-endothelial venules.Ciba Found Symp 71: 265–286, 1980
Cheung, P., Kao, F. T., Law, M. L., Jones, C., Puck, T. T., and Chan, L.: Localization of the structural gene for human apolipoprotein A-I on the long arm of human chromosome 11.Proc Natl Acad Sci USA 81: 508–511, 1984
D'Eustachio, P., Owens, G. C., Edelman, G. M., and Cunningham, B. A.: Chromosomal location of the gene encoding the neural cell adhesion molecule (N-CAM) in the mouse.Proc. Natl Acad Sci USA 82: 7631–7635, 1985
Douglas, T. C. and Dawson, D. W.: Location of the gene for theta antigen in the mouse. III. The position ofThy-1 relative toLap-1 andMpi-1.J Hered 70: 250–254, 1979
Eicher, E. M., Taylor, B. A., Leighton, S. C., and Womack, J.E.: A serum protein polymorphism determinant on chromosome 9 ofMus musculus.Mol Gen Genet 177: 571–576, 1980
Forsberg, U.H., Ala-Kapee, M. M., Jalkanen, S., Andersson L. C., and Schröder, J.: The gene for human lymphocyte homing receptor is located on chromosome 11.Eur J Immunol 19: 409–412, 1989a
Forsberg, U.H., Jalkanen, S., and Schröder, J.: Assignment of the human lymphocyte homing receptor gene to short arm of chromosome 11.Immunogenetics 29: 405–407, 1989b
Francke, U., Foellmar, B.E., and Haynes, B. F.: Chromosome mapping of human cell surface molecules: monoclonal anti-human lymphocyte antibodies 4F2, A3D8, and A1G3 define antigens controlled by different regions of chromosome 11.Somat Cell Genet 9: 333–344, 1983
Glaser, T., Housman, D., Lewis, W. H., Gerhard, D., and Jones, C.: A fine structure deletion map of human chromosome 11p: analysis of the J1 series hybrids.Somatic Cell Mol Genet, in press, 1989
Gold, D. P., Van Dongen, J. J. M., Morton, C. C., Bruns, G. A. P., van den Elsen, P., van Kessel, Ad H. M. G., and Terhorst, C.: The gene encoding the subunit of the T3/T-cell receptor complex maps to chromosome 11 in humans and to chromosome 9 in mice.Proc Natl Acad Sci USA 84: 1664–1668, 1987
Goodfellow, P. N., Tunnacliffe, A., Pym, B., Solomon, E., Walsh, F., Knowles, B., Andrews, P., Parkar, M., and Povey, S.: Multiple loci on chromosome 11 control expression of cell surface antigens.Cytogenet Cell Genet 37: 481, 1984
Hammelburger, J., Palfree, R. E., Sirlin, S., and Hämmerling, U.: Demonstration of phosphatidylinositol anchors on Ly-6 molecules by specific phospholipase C digestion and gel electrophoresis in octylglucoside.Biochem Biophys Res Commun 148: 1304–1311, 1987
Hiraki, S., Miyoshi, I., Kubonishi, L., Matsuda, Y., Nakayama, T., Kishimoto, H., and Masuji, H.: Human leukemic “null” cell line (NALL-1).Cancer 40: 2131–2135, 1977
Hogarth, P. M., McKenzie I. F. C., Sutton, V. R., Curnow, K. M., Lee, B. K., and Eicher, E. M.: Mapping of the mouseLy-6, Xp-14, andGdc-1 loci to chromosome 15.Immunogenetics 25: 21–27, 1987
Hořejší, V., Hilgert, I., Krištofová, H., Bažil, V., Bukovský, A., and Kulhánková, L.: Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-Microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a Pan-leucocyte antigen.Folia Biol (Praha) 32: 12–25, 1986.
Hořejší, V., Angelisová, P., Bažil, V., Krištofová, H., Stoyanov, S., Štefanová, I., Hausner, P., Vosecký, M. and Hilgert, I.: Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74).Folia Biol (Praha) 34: 23–34, 1988
Houlden, B. A., Hogarth, P. M., and McKenzie, I. F. C.: Interrelationships of the “Ly-6 complex” antigens.Immunogenetics 23: 226–232, 1986
Hyman, R. and Stallings, V.: Complementation patterns of Thy-1 variants and evidence that antigen loss variants “pre-exist” in the parental population.J Natn Cancer Inst 52: 429–436, 1974
Jalkanen, S., Bargatze, R. F., de los Toyos, J., and Butcher, E. C.: Lymphocyte recognition of high endothelium: antibodies to distinct epitopes of an 85–95-kD glycoprotein antigen differentially inhibit lymphocyte binding to lymph node, mucosal, or synovial endothelial cells.J Cell Biol 105: 983–990, 1987
Jones, C., Kao, F.-T.: Regional mapping of the gene for human lysosomal acid phosphatase (ACP2) using a hybrid clone panel containing segments of human chromosome 11.Hum Genet 45: 1–10, 1978
Jones, C., Bill, J., Larizza, L., Pym, B., Goodfellow, P., and Tunnacliffe, A. Relationship between genes on human chromosome 11 encoding cell surface antigen.Somat Cell Mol Genet 10: 423–428, 1984
Junien, C. and McBride, W.: Report of the committee on the genetic constitution of chromosome 11. Update to the ninth international workshop on human gene mapping.Cytogenet Cell Genet 49: 79–81, 1988
Jutila, M. A., Kroese, F. G. M., Jutila, K. L., Stall, A. M., Fierieng, S., Herzenberg, L. A., Berg, E. L., and Butcher, E. C.: Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon-gamma.Eur J Immunol 18: 1819–1826 1988
Kao, F.-T., and Puck, T. T.: Genetics of somatic mammalian cells. VII. Induction and isolation of nutritional mutants in Chinese hamster cells.Proc Natl Acad Sci USA 60: 1275–1281, 1968
Kao, F.-T., Jones, C., and Puck, T. T.: Genetics of somatic mammalian cells: genetic, immunologic, and biochemical analysis with Chinese hamster cell hybrids containing selected human chromosomes.Proc Natl Acad Sci USA 73: 193–197, 1976
Kao, F.-T., Jones, C., and Puck, T. T.: Genetics of cell surface antigens: regional mapping of three components of the human cell surface antigen complex, AL, on chromosome 11.Somat Cell Genet 3: 421–429, 1977
Karcher, D., Lowenthal, A., Thormar, H., and Hoppe, M.: Serological identification of viral antigens after electrophoretic transfer.J Immunol Methods 43: 175–180, 1981
Kazazian, H. H. J. and Junien, C.: Report of the committee on the genetic constitution of chromosome 10, 11 and 12. Ninth international workshop on human gene mapping.Cytogenet Cell Genet 46: 188–212, 1987
Kimura, S., Tada, N., Liu-Lam, Y., and Hämmerling, U.: Studies of the mouseLy-6 alloantigen system. II. Complexities of theLy-6 region.Immunogenetics 20: 47–56, 1984
Krissansen, G. W., Gorman, P. A., Kozak, C. A., Spurr, N. K., Sheer, D., Goodfellow, P. N., and Crumpton, M. J.: Chromosomal locations of the gene coding for the CD3 (T3) γ subunit of the human and mouse CD3/T-cell antigen receptor complexes.Immunogenetics 26: 258–266, 1987
Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature 227: 680–685, 1970
LeClair, K. P., Palfree, R. G. E., Flood, P. M., Hämmerling, U., and Bothwell A.: Isolation of murine Ly-6 cDNA reveals a new multigene family.EMBO J 5: 3227–3234, 1986
LeClair, K. P., Rabin, M., Nesbitt, M. N., Pravtcheva, D., Ruddle, F. H., Palfree, R. G. E., and Bothwell, A.: Murine Ly-6 family is located on chromosome 15.Proc Natl Acad Sci USA 84: 1638–1642, 1987
Lipinski, M., Parks, D. R., Rouse, R. V., and Herzenberg, L. A.: Human trophoblast cell-surface antigens defined by monoclonal antibodies.Proc. Natl Acad Sci USA 78: 5147–5150, 1981
Lu, L., Palladino, M. A., Jr., and DeLeo, A. B.: The immunotherapeutic effects in tumour-bearing mice of Ly-6 monoclonal antibodies.J Immunol 142: 719–725, 1989
Lyon, M. F.: Mouse chromsome atlas.Mouse News Letters 81: 20–41, 1988
Malek, T. R., Ortega, C., Kroczek, R., and Shevach, E. M.: Role of Ly-6 in lymphocyte activation. II. Induction of T cell activation by monoclonal anti-Ly-6 antibodies.J Exp Med 164: 709–722, 1986
Meisler, M. H., Wanner, L., Kao, F. T., and Jones, C.: Localization of the urophorphyrinogen I synthase locus to human chromosome region 11q13-ter and interconversion of charge isomers.Cytogenet Cell Genet 31: 124–128, 1981
Minowada, J., Janossy, G., Greaves, M., Tsubota, T., Srivastava B. I. S., Morikawa, S., and Tasumi, E.: Expression of an antigen associated with acute lymphoblastic leukemia in human leukemialymphoma cell lines.J Natn Cancer Inst 60: 1269, 1978
Nadeau, J. H., and Reiner, A. H.: Homology mapping database man on mouse.Mouse News letters 81: 43–44, 1988
Nguyen, C., Mattei, M. G., Goridis, C., Mattei, J. F., and Jordan, B. R.: Localization of the human N-CAM gene to chromosome 11 by in situ hybridization with a murine N-CAM cDNA probe.Cytogenet Cell Genet 40: 713, 1985
Nikinmaa, B., Gahmberg, C. G., and Schröder, J.: Assignment of gene coding for cell surface glycoprotein with a molecular weight of 75000 to human chromosome 11.Somat Cell Genet 9: 301–312, 1983
Otto, P., and von Deimling, O.: Esterase-17 (ES-17): characterization and linkage to chromosome 9 of a new bis-p-nitrophenyl phosphate resistant esterase of the house mouse (Mus musculus).Biochem Genet 21: 37–48, 1983
Palfree, R. G. E., Sirlin, S., Dumont, F. J., and Hämmerling, U.: N-terminal and cDNA characterization of murine lymphocyte antigen Ly-6C.2.J Immunol 140: 305–310, 1988
Picker, L. J., de los Toyos, J., Telen, M. J., Haynes, B. F., and Butcher, E. C.: Monoclonal antibodies against the CD44 (In(Lu)-related p80), and Pgp-1 antigens in man recognize the Hermes class of lymphocyte homing receptors.J Immunol 142: 2046–2051, 1989
Rettig, W. J., Dracopoli, N. C., Goetzer, T. A., Spengler, B. A., Biedler, J. L., Oettgen, H. F., and Old, L. J.: Somatic cell genetic analysis of human cell surface antigens: chromosomal assignments and regulation of expression in rodent human hybrids cells.Proc Natl Acad Sci USA 81: 6437–6441, 1984
Rettig, W. J., Dracopoli, N. C., Chesa, P. G., Spengler, B. A., Beresford, H. R., Davies, P., Biedler, J. L., and Old, L. J.: Role of human chromosome 11 in determining surface antigenic phenotype of normal and malignant cells.J Exp Med 162: 1603–1619, 1985
Rettig, W. J., Grzeschik, K.-H., Yenamandra, A. K., Garcia, E., and Old, L. J.: Definition of selectable cell surface markers for human chromosomes and chromosome segments in rodent-human hybrids.Somatic Cell Mol Genet 14: 223–231, 1988
Rock, K. L., Yeh, E. T. H., Gramm, C. F., Haber, S. I., Reiser, H., and Benacerraf, B.: TAP, a novel T cell-activating protein involved in the stimulation of MHC-restricted T lymphocytes.J Exp Med 163: 315–333, 1986
Schröder, J., Suomalainen, H. A., Parkkinen, E., Lundqvist, C., Wada, G., and Koskimies, S.: Specificity of monoclonal antibodies to an EBV transformed B-cell line.Med Biol 60: 255–259, 1982
Schröder, J., Nikinmaa, B., Kavathas, P., and Herzenberg, L. A.: Fluorescence-activated cell sorting of mouse-human hybrid cells aids in locating the gene for the Leu 7 (HNK-1) antigen to human chromosome 11.Proc Natl Acad Sci 80: 3421–3424, 1983
Seabright, M. A.: A rapid banding technique for human chromosomes.Lancet II: 971–972, 1971
Searle, A. G., Peters, J., Lyon, M. F., Evans, E. P., Edwards, J. H., and Buckle, V. J.: Chromosome maps of man and mouse, III.Genomics 1, 3–18, 1987
Seki, T., Spurr, N., Obata, F., Goyert, S., Goodfellow, P., and Silver, J.: The human Thy-1 gene: structure and chromosomal location.Proc Natl Acad Sci USA 82: 6657–6661, 1985
Štefanová, I., Hilgert, I., Krištofová, H., Brown, R., Low, M. G., and Hořejší, V.: Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol.Mol Immunol 26: 153–161, 1989a
Štefanová, I., Hilgert, I., Bažil, V., Angelisová, P., and Hořejší, V.: Reactivity of the nonlineage/NK panel antibodies with purified antigens CD45, CD43, CDw44, CD18 and three “novel” antigens MEM-43, MEM-53 and MEM 102.In W. Knapp (ed.).Leucocyte Typing IV, Oxford University Press, Oxford, in press, 1989b
Štefanová, I., Hilgert, I., Bažil, V., Krištofová, H., and Hořejší, V.: Human leucocyte surface glycoprotein CDw44 and lymphocyte homing receptor are identical molecules.Immunogenetics, 29: 402–404, 1989c
Suomalainen, H. A., Goldsby, R. A., Osborne, B. A., and Schröder, J.: Mouse/human T-cell hybrids rosetting with sheep erythrocytes.Scand J Immunol 11: 163–168, 1980
Towbin, H., Staehelin, T., and Gordon, J.: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedures and some application.Proc Natl Acad Sci USA 76: 4350–4354, 1979
van den Elsen, P., Bruns, G., Gerhard, D. S., Pravtcheva, D., Jones, C., Housman, D., Ruddle, F. A., Orkin, S., and Terhorst, C.: Assignment of the gene coding for the T3-subunit of the T3-T-cell receptor complex to the long arm of human chromosome 11 and to mouse chromosome 9.Proc Natl Acad Sci USA 82: 2920–2924, 1985
Wang, A., Arredondo-Vega, F. X., Giampietro, P. F., Smith, M., Anderson, W. F., and Desnick, R. J.: Regional gene assignment of human porphobilinogen deaminase and esterase A4 to chromosome 11q23-11qter.Proc Natl Acad Sci USA 78: 5734–5738, 1981
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Forsberg, U.H., Bažil, V., Štefanová, I. et al. Gene for human CD59 (likely Ly-6 homologue) is located on the short arm of chromosome 11. Immunogenetics 30, 188–193 (1989). https://doi.org/10.1007/BF02421205
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02421205