Summary
Previous studies have identified γ-carboxyglutamic acid as a constituent of one or more protein(s) synthesized by rat and chicken kidney microsomes in vitro in a vitamin K-dependent post-translational reaction [1]. Incubation of microsomes from a mouse kidney cell line (RAG) with [14C]NaHCO3 results in formation of protein-bound [14C]γ-carboxyglutamic acid. Incorporation is stimulated threefold by addition of the active vitamin K compound 2-methyl, 3-farnesyl, 1,4-naphthoquinone. At least 90% of incorporated, nondialyzable [14C] is situated in the γ-carboxyl group of γ-carboxyglutamic acid residues.
Similar content being viewed by others
References
Hauschka, P.V., Friedman, P.A., Gallop, P.M., Traverso, H.P.: Vitamin K-dependent γ-carboxyglutamic acid formation by kidney microsomesin vitro, Biochem. Biophys. Res. Commun.71:1207–1213, 1976
Shah, D.V., Suttie, G.W.: The vitamin K dependentin vitro production of prothrombin, Biochem. Biophys. Res. Commun.60:1397–1402, 1974
Esmon, C.T., Sadowski, G.A., Suttie, G.W.: A new carboxylation reaction. The vitamin K-dependent incorporation of H14CO3 − into prothrombin, J. Biol. Chem.250:4744–4748, 1975
Steflo, G., Suttie, G.W.: Vitamin K-dependent formation of γ-carboxyglutamic acid, Annu. Rev. Biochem.46:157–172, 1977
Hauschka, P.V., Lian, J.B., Gallop, P.M.: Direct identification of the calcium-binding amino acid, γ-carboxyglutamate, in mineralized tissue, Proc. Natl. Acad. Sci. U.S.A.72:3925–3929, 1975
Price, P.A., Otsuka, A.S., Poser, G.W., Kristaponis, J., Raman, N.: Characterization of a γ-carboxyglutamic acid-containing protein from bone, Proc. Natl. Acad. Sci. U.S.A.73:1447–1451, 1976
Traverso, H.P., Gallop, P.M., Hauschka, P.V.: Isolation of a vitamin K-dependent protein from chicken kidney microsomes containing γ-carboxyglutamic acid. XIth International Congress of Biochemistry, Toronto, 1979
Lian, J.B., Prien, E.L., Glimcher, M.J., Gallop, P.M.: The presence of protein-bound γ-carboxyglutamic acid in calcium-containing renal calculi, J. Clin. Invest.59:1151–1157, 1977
Lian, J.B., Skinner, M.S., Glimcher, M.J., Gallop, P.M.: The presence of γ-carboxyglutamic acid in the proteins associated with ectopic calcification, Biochem. Biophys. Res. Commun.73:349–355, 1976
Fernlund, P.: γ-Carboxyglutamic acid in human urine, Clin. Chim. Acta72:147–155, 1976
Friedman, P.A., Hauschka, P.V., Shia, M., Wallace, J.K.: Characteristics of the vitamin K-dependent carboxylation system in placenta, Biochim. Biophys. Acta583:261–265, 1979
Tuan, R.S.: Vitamin K-dependent γ-glutamyl carboxylase activity in the chick embryonic chorioallantoic membrane, J. Biol. Chem.254:1356–1364, 1979
Bell, R.G.: Vitamin K-dependent carboxylation in lung and other extrahepatic tissues, Fed. Proc.38:875, 1979
Traverso, H.P., Hauschka, P.V., Gallop, P.M.: Vitamin K-dependent carboxylation in microsomal preparations derived from cultured kidney cells, chick embryo fibroblasts, and pancreas, In Vitamin K Metabolism and Vitamin K-Dependent Proteins, J.W. Suttie, ed., University Park Press, Baltimore, pp. 311–314, 1979
Esmon, C.T., Suttie, J.W., Jackson, C.M.: Difference in phospholipid binding between normal and abnormal prothrombin, J. Biol. Chem.250:4095–4099, 1975
Nelsestuen, G.L., Lim, T.K.: Equilibrium involved in prothrombin- and blood-clotting factor X-membrane binding, Biochemistry16:4164–4171, 1977
Munns, T.W., Johnston, M.F.M., Liszewsky, M.K., Olson, R.E.: Vitamin K-dependent synthesis and modification of precursor prothrombin in cultured H-35 hepatoma cells, Proc. Natl. Acad. Sci. U.S.A.73:2803–2807, 1976
Lian, J.B., Friedman, P.A.: The vitamin K-dependent synthesis of γ-carboxyglutamic acid by bone microsomes, J. Biol. Chem.253:6623–6626, 1978
Nishimoto, S.K., Price, P.A.: Proof that the γ-carboxyglutamic acid-containing bone protein is synthesized in calf bone, J. Biol. Chem.254:437–441, 1979
Klebe, R.J., Tchaw-Ren Chen, Ruddle, F.H.: Controlled production of proliferating somatic cell hybrids, J. Cell Biol.45:74–82, 1970
Friedman, P.A., Shia, M.: Some characteristics of a vitamin K-dependent carboxylating system from rat liver microsomes, Biochem. Biophys. Res. Commun.72:647–654, 1976
Hauschka, P.V.: Quantitative determination of γ-carboxyglutamic acid in proteins, Anal. Biochem.80:212–223, 1977
Tishler, M., Fieser, L., Wendler, N.: Hydro, oxido and other derivatives of vitamin K1 and related compounds, J. Am. Chem. Soc.62:2866–2871, 1940
Suttie, G.W., Hageman, G.M., Lehrman, S.R., Rich, D.H.: Vitamin K-dependent carboxylase—Development of a peptide substrate, J. Biol. Chem.251:5826–5830, 1976
Hauschka, P.V., Reid, M.R.: Vitamin K dependence of a calcium-binding protein containing γ-carboxyglutamic acid in chicken bone, J. Biol. Chem.253:9063–9068, 1978
Orloff, J., Berliner, R.W. (eds.): Handbook of Physiology, Section 8: Renal Physiology. American Physiological Society, Washington, D.C., 1973
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Traverso, H.P., Hauschka, P.V. & Gallop, P.M. Vitamin K-dependent γ-carboxyglutamic acid formation by mouse renal adenocarcinoma cells (RAG). Calcif Tissue Int 30, 73–76 (1980). https://doi.org/10.1007/BF02408609
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02408609